AgaP_AGAP008096 (A. gambiae )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  AgaP_AGAP008096
Gene Name:  AGAP008096-PA
Original Clone ID:
8888z1BCt5p1
Keyword:
full-length cds
Species:
Anopheles gambiae
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified. Actual 5' flanking sequence provided below.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
AgCD00370050
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1308nts
Open reading frame: 46 to 1353

AGAATATTTTGTTTACTTTAAGAAGGAGATATACATATGTCCCTTAAAAAGAGCCCGCTG
ACCATTGTGCATTTAACGGATATCCACTATGACCCAGAATACGTTGTCGGCGTAAATGCG
GATTGCAAAGCCGAGGCATGTTGCCGTACTTTGCCTGACCTTGCTCCCGCGACACCGGAA
ACCGCCGCAGGTTATTGGTCTGATTACCGCGACTGTGATACGCCATGGCATGGGGTTGTG
GATGTCATGGAGCACATACGGAGACAGCATCCTAAAATTGACGCTATCTATTTTACTGGA
GATATTGTACACCATTTCACATGGAACACCAGTATCGAAACGAATGAAGGCGCGATGCGA
CAAGTGTTTGACCTCATGAAGCGTGTTTTCCCGGGTGTCCCCCTGTACCCGGTGCTGGGG
AACCACGAGTCACATCCAGCCAATTTATATGCACCTCACGATGTAGAAGCTTCCTACCGC
ATGGACTATTTGTATGATTTTATAGCGGATCAGTGGGCCGACTGGCTTCCGATTAAAGAT
ATCCGTCCCACCCTGACTGAGGGAGGCTACTATACAGTTCGCACGCCGTACGGTGTGCGG
ATTATTGGCCTCAACAATAACCCATGCTTCGTCCATAATTTTTGGCTGTTCTATTCGTTA
GACTACTTTATGCCTCAACTGCAGTGGTTGCACGATACCCTTCTGCAGGCAGAATGGGAT
AACGAACGTGTTCATATCCTCGCTCATGTACCGAGCTATGACGATTCTTGTTTCATAGGT
TGGACTCGCGAGTACAGAAAAATCGTGGAACGATTTGCGCACATTATTGCCGGGCAATTT
AATGGACATAGTCACGTCGACGAGTTCAACTTATATTATCGTCGCGATGACCCCTCACGG
GCAGTTTCCGTGGCGTGGAATGGCGGTTCGACAACCACGTTTACCAAGCTGAACCCAAAT
TACAAAGTATTCCAGTTTGATCCGGTCACTTTCGAACCTTTGGAACAAGAGACATGGATG
TATAACCTTACGGATGCTAATCTGACCCCGGACCAGCGTCCCAGCTGGTACCGCCTGTAT
TCTTTTAAAAGTTACTATCAGCTCCCAGATTTATCAGTTGACTCCCTGCATCGTTTGGTG
CAACGCTTCGCCCAGTCGGAACAACAGCTTCACAGATACTGGCAGGCAAAGCAAAAATAT
AGCGATCCGCTGCTGCAGGAGGGCTGCACTTCTACATGTCTCCGAAACGCGTTATGCGCT
ATAGTACGGACGGAACATGATGACGAACGTGCCTGTGAGTTGCTGTATGAACGCGCAGCG
CAAAGTGAAGGGCATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

KKSPLTIVHLTDIHYDPEYVVGVNADCKAEACCRTLPDLAPATPETAAGYWSDYRDCDTP
WHGVVDVMEHIRRQHPKIDAIYFTGDIVHHFTWNTSIETNEGAMRQVFDLMKRVFPGVPL
YPVLGNHESHPANLYAPHDVEASYRMDYLYDFIADQWADWLPIKDIRPTLTEGGYYTVRT
PYGVRIIGLNNNPCFVHNFWLFYSLDYFMPQLQWLHDTLLQAEWDNERVHILAHVPSYDD
SCFIGWTREYRKIVERFAHIIAGQFNGHSHVDEFNLYYRRDDPSRAVSVAWNGGSTTTFT
KLNPNYKVFQFDPVTFEPLEQETWMYNLTDANLTPDQRPSWYRLYSFKSYYQLPDLSVDS
LHRLVQRFAQSEQQLHRYWQAKQKYSDPLLQEGCTSTCLRNALCAIVRTEHDDERACELL
YERAAQSEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-8888z
Recommended expression in:
Not Applicable