liaS (B. subtilis )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  liaS
Gene Name:  sensor histidine kinase
Original Clone ID:
10013e1BCt17p1
Keyword:
full-length cds
Species:
Bacillus subtilis
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified. Actual 5' flanking sequence provided below.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
BsCD00370126
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 720nts
Open reading frame: 43 to 762

TATTTTGTTTACTTTAAGGAAGGAGATATACATATGTCCCTTAAACTTTCCAATGAACGT
GCGGAATGGCAGGCTCAAATGAAGAAGTCGGTTATCTCAGAAGAACGCCAGCGATTGGCC
AGAGATCTTCATGATGCGGTCAGCCAGCAGCTCTTTGCCATATCGATGATGACATCAGCC
GTGCTGGAACATGTCAAGGATGCTGATGACAAAACAGTCAAGCGGATCAGGATGGTCGAG
CATATGGCAGGCGAAGCCCAAAATGAGATGAGGGCGCTGCTGCTCCATTTACGGCCTGTT
ACCCTTGAAGGAAAAGGGCTGAAGGAGGGCCTTACGGAGCTTTTGGACGAGTTCCGAAAA
AAGCAGCCGATTGATATTGAGTGGGATATACAGGACACAGCGATATCCAAGGGTGTTGAA
GACCACTTGTTCAGAATCGTGCAGGAGGCCCTTTCAAACGTATTTAGACATTCAAAAGCG
TCAAAAGTAACCGTGATTCTGGGCATAAAGAACAGCCAGCTCCGTCTGAAGGTGATTGAT
AATGGAAAAGGCTTTAAAATGGACCAGGTGAAAGCCTCCTCATACGGCTTGAATTCTATG
AAAGAACGTGCAAGTGAAATCGGCGGTGTCGCCGAAGTGATTTCAGTAGAAGGAAAAGGC
ACTCAAATCGAAGTGAAGGTCCCGATTTTTCCGGAAGAAAAAGGAGAGAACGAACGTGAT
TCGAGTATTATTGATGAAGGGCATCATCACCATCACCACTGATCCGAATTCGAGCTCCGT
CGACAAGCTTGCGGCCGCACTCGAGCACCACCA

Protein sequence predicted based on nucleotide sequence

KLSNERAEWQAQMKKSVISEERQRLARDLHDAVSQQLFAISMMTSAVLEHVKDADDKTVK
RIRMVEHMAGEAQNEMRALLLHLRPVTLEGKGLKEGLTELLDEFRKKQPIDIEWDIQDTA
ISKGVEDHLFRIVQEALSNVFRHSKASKVTVILGIKNSQLRLKVIDNGKGFKMDQVKASS
YGLNSMKERASEIGGVAEVISVEGKGTQIEVKVPIFPEEKGENERDSSIIDEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-10013e
Recommended expression in:
Not Applicable