AFE_2796 (A. ferrooxidans ATCC 23270 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
AFE_0312
Keyword:
full-length cds
Species:
Acidithiobacillus ferrooxidans ATCC 23270
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
AfCD00370933
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 681nts
Open reading frame: 51 to 731

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGGAACTGC
CCAAAGTGGTCGCGTTGCTAGGGGTTCCGGCCCGTGCCTCCATGGCCTGGGCCCTGGTCG
GAGGCGACGCGTTACCCGCCAGTGAACTGGCCTACCGAGCAGGGGTGACCCCCCAAACCG
CCAGCCATCATTTGGCCCGGATGGTGGATGGTGGACTGCTTGCGGTGGAACGCTGTCTAC
GCTACCGCTACTATCGTTTGGCCAGTCCGGCGGTAGCCGAAATCCTGGAGAGTCTGATGG
CGTTGTCCGGCCCGCCGATCCTCAAGAACCGCCCAGACCGCGCCGCGGTGGATCCATTGC
GCCAAGCAAGGAGTTGTTACGATCATCTGGCGGGGAGTCTCGCGGTTGTCTTGGCGGATA
CCCTACAGCGCAACGGGTGGATCAACCTACAGGAGCGCGATTATCAGGTTACAGAGGTTG
GCGAACACGGATTCGCGGCCTTCGGGTTGGATCTGCCCGCCCTACGTCATCAGCAGCGGC
TTTTCGCGCGGCGCTGCATCGATTGGAGCGAGCGTCGCCCCCATATCGGCGGAGCGCTGG
GAGCCGCGTTGATGACGCAGTTCCAAGAGTCGGGATGGATACGGAGAAACCCCGGAGATC
GAAAAATCTGGGTTTCGAGCGCCGGCCAGCAAGGGCTGTGGAACACCTTTGGTATTGATG
CGACACCATAACGCGACTTAATTAATTTGGCGGATGAGAGAAGATTTTCAGCCTGATACA
GA

Protein sequence predicted based on nucleotide sequence

MELPKVVALLGVPARASMAWALVGGDALPASELAYRAGVTPQTASHHLARMVDGGLLAVE
RCLRYRYYRLASPAVAEILESLMALSGPPILKNRPDRAAVDPLRQARSCYDHLAGSLAVV
LADTLQRNGWINLQERDYQVTEVGEHGFAAFGLDLPALRHQQRLFARRCIDWSERRPHIG
GALGAALMTQFQESGWIRRNPGDRKIWVSSAGQQGLWNTFGIDATP*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g30a,L10L;g49t,A17S;
a81g,A27A;g84a,L28L;
g108a,R36R;g654c,K218N
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in 399034
Recommended expression in:
Not Applicable