AHA_1862 (A. hydrophila subsp. hydrophila ATCC 7966 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  AHA_1862
Gene Name:  response regulator protein
Original Clone ID:
11202f2BCt4p1
Keyword:
None
Species:
Aeromonas hydrophila subsp. hydrophila ATCC 7966
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified. Actual 5' flanking sequence provided below. High number of silent and/or missense mutations in CDS. Reference sequence corrected.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
AhCD00370973
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1266nts
Open reading frame: 51 to 1316

TCCCTCTCAATATTTTGTTAACTTTAGAAGGAGATATACATATGTCCCTTAAACAGAAAA
TATTGATAGTCGAGGATAGCATGACGATCCGTCGCATGTTGATCCAGGCCATCGCGCAGC
AAACTGGCCTTGAGATTGATGCCTTCGATACCCTGGAGGGGGCACGTCACTGCCAGGGAG
ACGAATACGTGGTCGCGCTGGTGGATCTCACCCTGCCTGATGCTCCCAGCGGGGAAGCCG
TCAAGGTGCTGCTGGAACGAGGCTTGCCAGTGGTGATCCTCACCGCCGACATCAGCGAAG
ACAAGCGGGAGGCCTGGCTGGAAGCAGGGGTGCTGGATTATGTGATGAAAGATTCACGTC
ACTCCCTGCAATACGCGGTCGGTCTGGTGCATCGCCTCTATCTCAATCAGCAGATTGAAG
TGCTGGTCGTCGATGATTCACGCACCTCCCGCCACCGCACCATGGCACAGCTACGCAAGC
AGTTGTTGCAGGTCCACGAGGCGAGCCATGCCCGCGAAGCCCTGGCCACCCTTGAGCAGC
ATCCGGCCATCCGGCTGGTACTGGTCGACTACTATATGCCGGAAATTGACGGCATCAGCC
TGGTGCGGATGCTGCGAGAGCGCTACAGCAAGCAGCAGCTCGCCATCATAGGGATCTCGG
TGTCGGACAAACGGGGCTTGTCTGCCCGCTACCTCAAGCAAGGGGCCAACGACTTTCTCA
ACCAGCCGTTCGAGCCGGAAGAGTTGCAGTGTCGGGTCAGCCATAACCTGGAGGCGCTTG
AGCAGTTCAACAGCATTCAGGAATCCGCCAACCGCGATTACCTGACCGGGCTTTATAACC
GGCGCTACTGGTTCAACGAGGGGCAAAAGTGGTTTGAGGAGCATCAGCGCCAGCAGACGC
CGCTCGCGCTGTGTGTCCTCGATGTGGATCACTTCAAGCAGGTCAACGATCACTGGGGTC
ATGCCATCGGCGATCAACTGCTCTGCCACCTCACCCGGTTGCTGACCCAGTTCTTCCCCG
ATGCCATGATTGCCCGCTTTGGGGGAGAGGAGTTGGCCCTTATGGTGCCCCATGGCACAG
TGCCAGTGCTGCTCAAACGGCTGGAGGGACGGCGCCAACAACTGCGTCAGCAACCCTTGT
CACGGGAGACGCCGCTGTTCGTTCGCGCCAGTTTCGGGGTGATCAATGTCGGGGGAGTCT
CCATGGAGGAGGCACTGAGGGAAGCGGATCGCCTGCTCTATCAGGCAAAAAACACAGGTC
GGGACAAGATAGTGTCGCAGGACTCATCGGAAGGGCATCATCACCATCACCACTGATCGG
AAT

Protein sequence predicted based on nucleotide sequence

KQKILIVEDSMTIRRMLIQAIAQQTGLEIDAFDTLEGARHCQGDEYVVALVDLTLPDAPS
GEAVKVLLERGLPVVILTADISEDKREAWLEAGVLDYVMKDSRHSLQYAVGLVHRLYLNQ
QIEVLVVDDSRTSRHRTMAQLRKQLLQVHEASHAREALATLEQHPAIRLVLVDYYMPEID
GISLVRMLRERYSKQQLAIIGISVSDKRGLSARYLKQGANDFLNQPFEPEELQCRVSHNL
EALEQFNSIQESANRDYLTGLYNRRYWFNEGQKWFEEHQRQQTPLALCVLDVDHFKQVND
HWGHAIGDQLLCHLTRLLTQFFPDAMIARFGGEELALMVPHGTVPVLLKRLEGRRQQLRQ
QPLSRETPLFVRASFGVINVGGVSMEEALREADRLLYQAKNTGRDKIVSQDSSEGHHHHH
H*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in NYSGXRC-11202f
Recommended expression in:
Not Applicable