DNASU Plasmid | PPM1E (H. sapiens )<br>In pFASTBAC1-C-His-TOPO (His-tagged baculovirus expression vector)

Explanation of Terms

Gene:

Gene Symbol: PPM1E

Gene Name: protein phosphatase, Mg2+/Mn2+ dependent, 1E

Original Clone ID:

8668a9KWt3

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pFASTBAC1-C-His-TOPO

Format:

CLOSED

Source:

New York SGX Research Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
New York SGX Research Center for Structural Genomics

HsCD00370997

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 945nts

Open reading frame: 51 to 995

Reference Sequence Alignment

GTCCCACCATCGGGCGCGGATCCCGGTCCGAAGCGCGCGGAATTCGCACCATGGCCCTTG
AAGGGACTGTGGAGATTGAGACAGTGAAATTGGCCCGTTCTGTCTTCAGCAAACTACACG
AGATTTGCTGCAGCTGGGTGAAAGACTTCCCCCTCCGCAGGAGACCCCAGCTTTATTATG
AGACATCAATCCATGCCATCAAAAACATGCGCAGGAAAATGGAGGACAAACATGTCTGCA
TTCCTGACTTTAATATGCTCTTCAACCTAGAGGACCAGGAAGAACAAGCTTACTTTGCAG
TGTTTGATGGCCATGGGGGAGTAGATGCTGCTATTTATGCCTCCATTCACCTCCACGTTA
ACTTAGTCCGCCAGGAGATGTTCCCCCATGATCCTGCTGAGGCCCTGTGCAGGGCCTTCC
GGGTCACTGATGAGCGGTTTGTGCAGAAAGCAGCCAGGGAGAGCTTAAGATGTGGGACCA
CAGGAGTGGTGACTTTCATCAGAGGCAACATGCTACATGTGGCCTGGGTGGGTGATTCCC
AGGTTATGCTTGTGAGAAAGGGCCAAGCTGTTGAACTAATGAAGCCACACAAACCAGACA
GAGAGGATGAAAAGCAGAGAATTGAGGCCCTTGGAGGTTGCGTAGTCTGGTTTGGTGCCT
GGAGGGTGAATGGAAGTCTGTCGGTTTCCAGAGCTATTGGAGATGCTGAACATAAGCCAT
ATATCTGTGGGGATGCAGATTCTGCCTCCACTGTTCTGGATGGGACCGAAGACTACCTCA
TTCTGGCCTGTGATGGCTTCTATGACACCGTGAACCCTGATGAGGCAGTGAAAGTTGTGT
CCGACCACCTGAAAGAGAATAATGGAGACAGCAGCATGGTTGCCCACAAATTAGTGGCAT
CAGCTCGTGATGCTGGGTCAAGTGATAACATCACGGTTATTGTGGTATTCCTGAGGGACA
TGAACAAAGAAGGGCATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

MALEGTVEIETVKLARSVFSKLHEICCSWVKDFPLRRRPQLYYETSIHAIKNMRRKMEDK
HVCIPDFNMLFNLEDQEEQAYFAVFDGHGGVDAAIYASIHLHVNLVRQEMFPHDPAEALC
RAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDSQVMLVRKGQAVELMKPH
KPDREDEKQRIEALGGCVVWFGAWRVNGSLSVSRAIGDAEHKPYICGDADSASTVLDGTE
DYLILACDGFYDTVNPDEAVKVVSDHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVF
LRDMNKEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin
Host Type: insect cells Marker: gentamicin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Not_Tested

ProteinPurified: Not_Tested

SolubleProtein: Not_Tested

Find experimental protocols in NYSGXRC-8668a

Recommended expression in:

Not Applicable

Vector Name:

pFASTBAC1-C-His-TOPO

Synonyms:

pFB1-C-His-TOPO, pFB1-C-His-TOPO_KW

Sequencing Primer:

Forward: pFB forward

Reverse: pFB reverse

Description:

Baculovirus vector, adds C-terminal 6xHis tag and TEV protease site; amp resistance; TOPO cloning.

Comments:

None

Size (bp):

4763

Parent Vector:

None

Properties:

TOPO Cloning, baculovirus/insect cell expression, with tag/fusion/marker

Author Name:

New York SGX Research Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

GACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCAGACCAGCCGCGTAACCTGGCAAAATCGGTTACGGTTGAGTAATAAATGGATGCCCTGCGTAAGCGGGTGTGGGCGGACAATAAAGTCTTAAACTGAACAAAATAGATCTAAACTATGACAATAAAGTCTTAAACTAGACAGAATAGTTGTAAACTGAAATCAGTCCAGTTATGCTGTGAAAAAGCATACTGGACTTTTGTTATGGCTAAAGCAAACTCTTCATTTTCTGAAGTGCAAATTGCCCGTCGTATTAAAGAGGGGCGTGGCCAAGGGCATGGTAAAGACTATATTCGCGGCGTTGTGACAATTTACCGAACAACTCCGCGGCCGGGAAGCCGATCTCGGCTTGAACGAATTGTTAGGTGGCGGTACTTGGGTCGATATCAAAGTGCATCACTTCTTCCCGTATGCCCAACTTTGTATAGAGAGCCACTGCGGGATCGTCACCGTAATCTGCTTGCACGTAGATCACATAAGCACCAAGCGCGTTGGCCTCATGCTTGAGGAGATTGATGAGCGCGGTGGCAATGCCCTGCCTCCGGTGCTCGCCGGAGACTGCGAGATCATAGATATAGATCTCACTACGCGGCTGCTCAAACCTGGGCAGAACGTAAGCCGCGAGAGCGCCAACAACCGCTTCTTGGTCGAAGGCAGCAAGCGCGATGAATGTCTTACTACGGAGCAAGTTCCCGAGGTAATCGGAGTCCGGCTGATGTTGGGAGTAGGTGGCTACGTCTCCGAACTCACGACCGAAAAGATCAAGAGCAGCCCGCATGGATTTGACTTGGTCAGGGCCGAGCCTACATGTGCGAATGATGCCCATACTTGAGCCACCTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACGGCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACAAAAAAACAGTCATAACAAGCCATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACAGTTTACGAACCGAACAGGCTTATGTCAACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGGACTCTAGCTATAGTTCTAGTGGTTGGCTACGTATACTCCGGAATATTAATAGATCATGGAGATAATTAAAATGATAACCATCTCGCAAATAAATAAGTATTTTACTGTTTTCGTAACAGTTTTGTAATAAAAAAACCTATAAATATTCCGGATTATTCATACCGTCCCACCATCGGGCGCGGATCCCGGTCCGAAGCGCGCGGAATTCGCACCATGGCCCTTAAGGGCATCATCACCATCACCACTGATAGCTCGAGGCATGCGGTACCAAGCTTGTCGAGAAGTACTAGAGGATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCTGATCACTGCTTGAGCCTAGGAGATCCGAACCAGATAAGTGAAATCTAGTTCCAAACTATTTTGTCATTTTTAATTTTCGTATTAGCTTACGACGCTACACCCAGTTCCCATCTATTTTGTCACTCTTCCCTAAATAATCCTTAAAAACTCCATTTCCACCCCTCCCAGTTCCCAACTATTTTGTCCGCCCACAGCGGGGCATTTTTCTTCCTGTTATGTTTTTAATCAAACATCCTGCCAACTCCATGTGACAAACCGTCATCTTCGGCTACTTTTTCTCTGTCACAGAATGAAAATTTTTCTGTCATCTCTTCGTTATTAATGTTTGTAATTGACTGAATATCAACGCTTATTTGCAGCCTGAATGGCGAATGG

Vector Features:

Type

Name

Description

Start

End

ATG start

start

initiation ATG (Met, Ala, Leu)

4065

4078

TOPO recognition site

TOPO

TOPO cloning site

4069

4078

bacterial origin

ori

pUC-type bacterial origin of replication

1544

2226

poly-A signal

polyA

SV40 polyA signal sequence

4189

4380

primer

pFB foward

forward sequencing primer: �TATTCCGGATTATTCATACC

3995

4014

primer

pFB reverse

reverse sequencing primer: ACAAATGTGGTATGGCTGATT

4150

4170

promoter

polyhedrin promoter for insect cell expression

3904

4032

selectable marker

GenR

gentamycin resistance gene

3000

3533

selectable marker

AmpR

ampicillin resistance gene

787

1446

ssDNA origin

f1 ori

f1 origin

325

tag

His

C-terminal 6xHis and stop

4079

4096

transposon fragment

Tn7L

Tn7L transposon site

1466

transposon fragment

Tn7R

Tn7R transposon site

2709

2933

Gene

Gene Symbol :

PPM1E

Symbol Nomenclature :

PPM1E

SYNONYM :

CaMKP-N; POPX1; PP2CH; caMKN

Designation :

Full Nomenclature :

protein phosphatase, Mg2+/Mn2+ dependent, 1E

GENEID :

22843

GI :

30089948

GenBank Accession :

NM_014906

HGNC :

19322

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00370997      ------------------------------------------------------------
NM_014906.4       ATGGCCGGCTGCATCCCTGAGGAGAAAACTTACCGGCGCTTCCTGGAGCTATTCCTGGGC
                                                                              

HsCD00370997      ------------------------------------------------------------
NM_014906.4       GAGTTTCGCGGACCGTGCGGCGGCGGCGAGCCGGAGCCGGAACCCGAACCCGAACCCGAA
                                                                              

HsCD00370997      ------------------------------------------------------------
NM_014906.4       CCCGAACCCGAGTCCGAGCCCGAGCCCGAACCTGAACTGGTAGAAGCTGAGGCGGCCGAG
                                                                              

HsCD00370997      ------------------------------------------------------------
NM_014906.4       GCTTCGGTAGAGGAACCCGGGGAGGAGGCGGCCACGGTAGCCGCGACGGAGGAGGGGGAC
                                                                              

HsCD00370997      ------------------------------------------------------------
NM_014906.4       CAGGAGCAAGACCCGGAGCCCGAGGAGGAGGCGGCGGTTGAGGGTGAGGAGGAGGAGGAG
                                                                              

HsCD00370997      ------------------------------------------------------------
NM_014906.4       GGCGCGGCGACGGCGGCGGCAGCCCCGGGGCACTCGGCCGTGCCGCCGCCGCCGCCCCAG
                                                                              

HsCD00370997      ------------------------------------------------------------
NM_014906.4       CTGCCGCCTTTGCCCCCGCTCCCGCGACCGCTGTCAGAGCGCATCACCCGCGAGGAGGTG
                                                                              

HsCD00370997      ------------------------------------------------------------
NM_014906.4       GAGGGCGAAAGCCTGGACCTGTGCCTGCAGCAGCTCTACAAATATAATTGCCCTTCCTTT
                                                                              

HsCD00370997      -------------------------------GTCCCACCATCGGGCGCGGATCCCGGTCC
NM_014906.4       TTGGCTGCTGCTTTAGCCAGAGCCACATCAGATGAAGTCCTTCAGAGTGATCTTTCTGCA
                                                 .* ... *.*  .*.* *.:       *.

HsCD00370997      GAAGCGCGCGGAATTCGCACCATGGCCCTTGAAGGGACTGTGGAGATTGAGACAGTGAAA
NM_014906.4       CATTATATCCCAAAGGAAACGGATGGCACAGAAGGGACTGTGGAGATTGAGACAGTGAAA
                   *: . . *  **:  ..** .: * *. :******************************

HsCD00370997      TTGGCCCGTTCTGTCTTCAGCAAACTACACGAGATTTGCTGCAGCTGGGTGAAAGACTTC
NM_014906.4       TTGGCCCGTTCTGTCTTCAGCAAACTACACGAGATTTGCTGCAGCTGGGTGAAAGACTTC
                  ************************************************************

HsCD00370997      CCCCTCCGCAGGAGACCCCAGCTTTATTATGAGACATCAATCCATGCCATCAAAAACATG
NM_014906.4       CCCCTCCGCAGGAGACCCCAGCTTTATTATGAGACATCAATCCATGCCATCAAAAACATG
                  ************************************************************

HsCD00370997      CGCAGGAAAATGGAGGACAAACATGTCTGCATTCCTGACTTTAATATGCTCTTCAACCTA
NM_014906.4       CGCAGGAAAATGGAGGACAAACATGTCTGCATTCCTGACTTTAATATGCTCTTCAACCTA
                  ************************************************************

HsCD00370997      GAGGACCAGGAAGAACAAGCTTACTTTGCAGTGTTTGATGGCCATGGGGGAGTAGATGCT
NM_014906.4       GAGGACCAGGAAGAACAAGCTTACTTTGCAGTGTTTGATGGCCATGGGGGAGTAGATGCT
                  ************************************************************

HsCD00370997      GCTATTTATGCCTCCATTCACCTCCACGTTAACTTAGTCCGCCAGGAGATGTTCCCCCAT
NM_014906.4       GCTATTTATGCCTCCATTCACCTCCACGTTAACTTAGTCCGCCAGGAGATGTTCCCCCAT
                  ************************************************************

HsCD00370997      GATCCTGCTGAGGCCCTGTGCAGGGCCTTCCGGGTCACTGATGAGCGGTTTGTGCAGAAA
NM_014906.4       GATCCTGCTGAGGCCCTGTGCAGGGCCTTCCGGGTCACTGATGAGCGGTTTGTGCAGAAA
                  ************************************************************

HsCD00370997      GCAGCCAGGGAGAGCTTAAGATGTGGGACCACAGGAGTGGTGACTTTCATCAGAGGCAAC
NM_014906.4       GCAGCCAGGGAGAGCTTAAGATGTGGGACCACAGGAGTGGTGACTTTCATCAGAGGCAAC
                  ************************************************************

HsCD00370997      ATGCTACATGTGGCCTGGGTGGGTGATTCCCAGGTTATGCTTGTGAGAAAGGGCCAAGCT
NM_014906.4       ATGCTACATGTGGCCTGGGTGGGTGATTCCCAGGTTATGCTTGTGAGAAAGGGCCAAGCT
                  ************************************************************

HsCD00370997      GTTGAACTAATGAAGCCACACAAACCAGACAGAGAGGATGAAAAGCAGAGAATTGAGGCC
NM_014906.4       GTTGAACTAATGAAGCCACACAAACCAGACAGAGAGGATGAAAAGCAGAGAATTGAGGCC
                  ************************************************************

HsCD00370997      CTTGGAGGTTGCGTAGTCTGGTTTGGTGCCTGGAGGGTGAATGGAAGTCTGTCGGTTTCC
NM_014906.4       CTTGGAGGTTGCGTAGTCTGGTTTGGTGCCTGGAGGGTGAATGGAAGTCTGTCGGTTTCC
                  ************************************************************

HsCD00370997      AGAGCTATTGGAGATGCTGAACATAAGCCATATATCTGTGGGGATGCAGATTCTGCCTCC
NM_014906.4       AGAGCTATTGGAGATGCTGAACATAAGCCATATATCTGTGGGGATGCAGATTCTGCCTCC
                  ************************************************************

HsCD00370997      ACTGTTCTGGATGGGACCGAAGACTACCTCATTCTGGCCTGTGATGGCTTCTATGACACC
NM_014906.4       ACTGTTCTGGATGGGACCGAAGACTACCTCATTCTGGCCTGTGATGGCTTCTATGACACC
                  ************************************************************

HsCD00370997      GTGAACCCTGATGAGGCAGTGAAAGTTGTGTCCGACCACCTGAAAGAGAATAATGGAGAC
NM_014906.4       GTGAACCCTGATGAGGCAGTGAAAGTTGTGTCCGACCACCTGAAAGAGAATAATGGAGAC
                  ************************************************************

HsCD00370997      AGCAGCATGGTTGCCCACAAATTAGTGGCATCAGCTCGTGATGCTGGGTCAAGTGATAAC
NM_014906.4       AGCAGCATGGTTGCCCACAAATTAGTGGCATCAGCTCGTGATGCTGGGTCAAGTGATAAC
                  ************************************************************

HsCD00370997      ATCACGGTTATTGTGGTATTCCTGAGGGACATGAACAAAGAAGGGCATCATCACCATCAC
NM_014906.4       ATCACGGTTATTGTGGTATTCCTGAGGGACATGAACAAAGCTGTAAATGTTAGTGAGGAA
                  ****************************************.:* ..** :*..  *  *.

HsCD00370997      CACTGA------------------------------------------------------
NM_014906.4       TCAGATTGGACAGAGAACTCTTTTCAAGGAGGGCAAGAAGATGGTGGGGATGATAAGGAG
                   .. .:                                                      

HsCD00370997      ------------------------------------------------------------
NM_014906.4       AATCATGGAGAGTGCAAACGCCCTTGGCCTCAGCACCAGTGCTCAGCACCAGCCGACCTA
                                                                              

HsCD00370997      ------------------------------------------------------------
NM_014906.4       GGCTATGATGGGCGTGTGGATTCATTCACTGATAGAACTAGCCTGAGCCCAGGGTCCCAA
                                                                              

HsCD00370997      ------------------------------------------------------------
NM_014906.4       ATCAACGTGCTGGAAGACCCAGGCTACCTAGATCTCACACAAATAGAAGCAAGCAAACCT
                                                                              

HsCD00370997      ------------------------------------------------------------
NM_014906.4       CACAGTGCCCAGTTTTTGCTACCAGTTGAGATGTTTGGTCCTGGTGCACCAAAGAAAGCA
                                                                              

HsCD00370997      ------------------------------------------------------------
NM_014906.4       AATCTTATTAATGAGTTAATGATGGAGAAAAAATCAGTTCAGTCATCATTGCCTGAATGG
                                                                              

HsCD00370997      ------------------------------------------------------------
NM_014906.4       AGTGGTGCTGGAGAGTTTCCCACTGCTTTCAATTTGGGTTCAACAGGGGAGCAGATATAC
                                                                              

HsCD00370997      ------------------------------------------------------------
NM_014906.4       AGAATGCAGAGCTTGTCTCCTGTCTGTTCAGGGTTGGAAAATGAACAGTTCAAATCCCCG
                                                                              

HsCD00370997      ------------------------------------------------------------
NM_014906.4       GGAAACAGAGTTTCTAGATTGTCTCATTTACGCCACCACTACTCAAAGAAGTGGCACAGA
                                                                              

HsCD00370997      ------------------------------------------------------------
NM_014906.4       TTCAGGTTTAATCCAAAGTTTTATTCATTTCTCTCTGCTCAAGAGCCTTCCCACAAAATA
                                                                              

HsCD00370997      ------------------------------------------------------------
NM_014906.4       GGCACTAGCCTGTCCTCACTTACTGGAAGTGGGAAGAGAAATAGGATAAGAAGTTCTCTG
                                                                              

HsCD00370997      ------------------------------------------------------------
NM_014906.4       CCATGGAGGCAAAATAGTTGGAAAGGGTACAGTGAAAACATGAGGAAGCTCAGAAAGACT
                                                                              

HsCD00370997      ------------------------------------------------
NM_014906.4       CATGATATTCCATGCCCAGATCTTCCTTGGAGCTATAAAATAGAATAA