SSH2 (H. sapiens )
In pFASTBAC1-C-His-TOPO (His-tagged baculovirus expression vector)

Explanation of Terms

Gene:
Gene Symbol:  SSH2
Gene Name:  slingshot protein phosphatase 2
Original Clone ID:
8688a4KWt1
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pFASTBAC1-C-His-TOPO
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
HsCD00371000
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 471nts
Open reading frame: 51 to 521

GTCCCACCATCGGGCGCGGATCCCGGTCCGAAGCGCGCGGAATTCGCACCATGGCCCTTG
ATAGCCCTACACAGATATTTGAGCATGTGTTCCTGGGCTCAGAATGGAATGCCTCCAACT
TAGAGGACTTACAGAACCGAGGGGTACGGTATATCTTGAATGTCACTCGAGAGATAGATA
ACTTCTTCCCAGGAGTCTTTGAGTATCATAACATTCGGGTATATGATGAAGAGGCAACGG
ATCTCCTGGCGTACTGGAATGACACTTACAAATTCATCTCTAAAGCAAAGAAACATGAAT
CTAAATGCCTTGTGCACTGCAAAATGGGGGTGAGTCGCTCAGCCTCCACCGTGATTGCCT
ATGCAATGAAGGAATATGGCTGGAATCTGGACCGAGCCTATGACTATGTGAAAGAAAGAC
GAACGGTAACCAAGCCCAACCCAAGCTTCATGAGACAACTGGAAGAGTATCAGGGGATCT
TGCTGGCAAGCAAAGAAGGGCATCATCACCATCACCACTGATAGCTCGAGGCATGCGGTA
CCAAGCTTGTCGAGAAGTACTAGAGGATCATA

Protein sequence predicted based on nucleotide sequence

MALDSPTQIFEHVFLGSEWNASNLEDLQNRGVRYILNVTREIDNFFPGVFEYHNIRVYDE
EATDLLAYWNDTYKFISKAKKHESKCLVHCKMGVSRSASTVIAYAMKEYGWNLDRAYDYV
KERRTVTKPNPSFMRQLEEYQGILLASKEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
G385E

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  insect cells    Marker:  gentamicin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Tested
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
Find experimental protocols in NYSGXRC-8688a
Recommended expression in:
Not Applicable