DNASU Plasmid | TM0643 (T. maritima ) in pMH2T7 (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TM0643

Gene Name: clostripain-related protein

Original Clone ID:

8126

Keyword:

full-length cds

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH2T7

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

Linker not verified.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00382413

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1407nts

Open reading frame: 36 to 1442

ATGGGATCTGATAAAATTCATCATCATCATCATCATTGAGATGGTTTCTTTTGATTGTGG
TTTCTGCTCTTTTTCTTTCTGGATGTATGCCGGAGATCTACACACACGCGTCCACACCTT
CCACAATAATCGCGGATAATGAAACCCACATCGCTGGGAGATACGCTTTTGTGGACGTTC
AGGTGCTCGACGAGTACGGTCTTCCCGTCTCAGGTGAGGATGTTTCTTTCTATGTGGATG
GTGCCTTCCTTGGAGAAGCAACCACCGACACAAGCGGTGTAGCAAGAATAGGTTTTTTCT
CTCCATCGGAGAAAACTTACACATTCACAGCGGTGGCAGGTGGTGTGAGCAGGTCTTTTG
ATGTTAGTTTCACAAAACCGAAGTGGCTTTTCATCGTGTGGATGGCAGCAGACAACAATC
TTTATTACTACTCCGAGGATGACCTTTCCGAGATGAGAAACGCCAGTGGAAGTGTTTCTG
TGGTTGTTGTCTACGACGGGATAGGGATCGGCGATGGAATGCTGGTGCTCGATGAGAGTG
GAAACTTTCAGGCTGTTGTCGGAACGATGGGGATAGACTTCAACAGTGGATCGTACACAA
ATCTGGAAGCCTGGCTTGAGATGATTCTCAATCAATTCGATGCCGACCATTACGCCCTTG
TGATCTGGGATCACGGGAGTGCGTGGATCGGTGACTCATACTACATCTCAACGAAGGTGA
TCGGATACGATGACTTTCAAGGAACAGCCATCGCTGTTTCGAACCTGAGGAAAGCACTTG
AAAACGCTCTTTCTGGAGACAAACTCGACATCCTCGGGTTTGACGCGTGTCTCATGGGAT
CTCTTGAGGTGATATACGAACTCAGAAACACAGCAGATTATATCGTGGCATCAAGTTTCA
ACGAGCCAGCTGAAGGATGGGATTATTCCTTTCTTGGTGAGATAGCACCAGATAGCACCC
CACTAGACGTGGCAAGGATGATAGTTGACAGTTACAGAGAGTACTATTCCTCTGATACTT
ATCAGAACGAACTGAGCCTTGCGGTGTACGACACCTCGCAGGTGGAGGTGTTTGTTTCTG
ATTTAAACTTGTTCATCAGTGATCTGAAAGCAGATCTGAGCAAGGTCGATGCGGTTTATT
CTGATGTGGTGAAGAGTTATATTGACGATTACAATCAGACAGTTCTTGTTGATCTTGGGG
ATTTCATAGATAGATGGGTGTCATCGTCAAGGATCACTACTGCTCCAGATTTGAGCTCTG
TTGTCGTTTATTCCTACGGTGAGATAGCCGGCAAGACACTCTCTTTTCCCATCAGTATCT
TCATGCCAGAAAGCATGTCGTGTTATCAGGACTATCAGACAGACTATCAGACACTTTCCT
TTCCGTATGACACTCAGTGGGACGAGTTTCTGGAATACTGGTTAAACCAAAAAGGCGGCT
AATTAATAA

Protein sequence predicted based on nucleotide sequence

LRWFLLIVVSALFLSGCMPEIYTHASTPSTIIADNETHIAGRYAFVDVQVLDEYGLPVSG
EDVSFYVDGAFLGEATTDTSGVARIGFFSPSEKTYTFTAVAGGVSRSFDVSFTKPKWLFI
VWMAADNNLYYYSEDDLSEMRNASGSVSVVVVYDGIGIGDGMLVLDESGNFQAVVGTMGI
DFNSGSYTNLEAWLEMILNQFDADHYALVIWDHGSAWIGDSYYISTKVIGYDDFQGTAIA
VSNLRKALENALSGDKLDILGFDACLMGSLEVIYELRNTADYIVASSFNEPAEGWDYSFL
GEIAPDSTPLDVARMIVDSYREYYSSDTYQNELSLAVYDTSQVEVFVSDLNLFISDLKAD
LSKVDAVYSDVVKSYIDDYNQTVLVDLGDFIDRWVSSSRITTAPDLSSVVVYSYGEIAGK
TLSFPISIFMPESMSCYQDYQTDYQTLSFPYDTQWDEFLEYWLNQKGG*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Protein_Purified

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in 282515

Recommended expression in:

Not Applicable

Vector Name:

pMH2T7

Synonyms:

pMH-2-T7

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.

Comments:

None

Size (bp):

4084

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTAATTAATTAACTTTAAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColEI origin of replication

1921

2603

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

selectable marker

AmpR

ampicillin resistance gene

1164

1823

tag

His

N-terminal 6x His tag

367

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

Gene

Gene Symbol :

TM0643

GENEID :

897747

Locus Tag :

TM0643

GI :

15643408

GenBank Accession :

AAD35727

Target GenBank:

NP_228452

Protein

SMART domain :

BID_1 : Bacterial Ig-like domain (group 1)

Structural Annotation Wiki :

TOPSAN

UniProt :

Q9WZB1

Clone

Cloning Information :

276072

HIP Master Clone ID :

205558

Original Clone ID :

8126

Experimental

TargetTrack - Experimental data :

282515

Organism

Species Specific ID:

897747

Reagents

Labome :

TM0643-cDNA