DNASU Plasmid | thiH (T. maritima ) in pMH2T7 (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: thiH

Gene Name: thiamine biosynthesis protein ThiH

Original Clone ID:

7662

Keyword:

full-length cds

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH2T7

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

Linker not verified.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00382471

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1422nts

Open reading frame: 48 to 1469

GATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCATGTGTATGTATG
TGTTTGTGAAAGAGCGTGTAGAGAGCAGATCTTTCATACCGGAAGAAAAGATATTTGAAC
TTCTGGAGAAAACGAAAAACCCGGATCCTGCAAGGGTGAGAGAGATCATCCAGAAGTCGC
TGGACAAGAACAGGCTCGAGCCGGAAGAGACGGCCACCCTTTTGAATGTGGAAGATCCAG
AGCTTCTGGAGGAGATCTTCGAAGCAGCCCGCACTCTGAAAGAGAGAATCTACGGAAACA
GAATCGTTCTTTTTGCACCACTCTACATAGGAAACGATTGTATAAACGACTGTGTTTACT
GCGGTTTCAGAGTTTCCAACAAAGTGGTGGAAAGAAGAACGCTCACAGAAGAGCAGTTGA
AAGAAGAAGTCAAAGCGCTCGTTTCCCAGGGACACAAAAGGCTCATCGTTGTCTATGGAG
AGCACCCCAATTACTCACCTGAGTTCATCGCAAGAACGATCGACATCGTTTACAACACGA
AGTACGGAAACGGTGAGATCAGACGCGTGAACGTCAACGCGGCACCTCAAACGATTGAAG
GCTACAAGATCATAAAGTCCGTGGGAATCGGCACTTTCCAGATCTTTCAGGAAACGTACC
ACAGAGAAACTTACCTGAAGCTTCACCCAAGAGGCCCGAAATCGAACTACAACTGGAGGC
TCTACGGTCTGGACAGGGCGATGATGGCGGGAATCGACGACGTTGGAATAGGAGCGCTCT
TCGGCCTCTACGACTGGAAGTTCGAAGTGATGGGTCTCCTTTACCACACGATACACCTCG
AAGAGAGATTCGGCGTAGGACCGCACACCATCTCCTTCCCGAGGATAAAACCCGCGATAA
ACACACCATATTCGCAGAAACCGGAACACGTCGTGAGCGATGAAGACTTCAAGAAGCTCG
TCGCCATCATAAGGCTTTCTGTTCCGTACACGGGAATGATTCTCACCGCGAGAGAACCCG
CAAAACTCAGAGACGAGGTGATAAAGCTTGGTGTCTCACAGATAGACGCCGGCTCCAGAA
TAGGAATCGGGGCTTACTCTCACAAGGAAGACGACGAAGACAGAAAGAGACAGTTCACGC
TCGAAGACCCAAGGCCCCTCGATCAGGTGATGAGAAGCCTTCTGAAAGAAGGTTTTGTAC
CGTCATTCTGCACCGCGTGCTACAGGGCTGGTAGAACGGGAGAGCACTTCATGGAGTTTG
CGATTCCCGGTTTCGTGAAGAACTTCTGCACACCGAACGCCCTGTTCACACTACAGGAGT
ACCTCTGCGACTACGCGACCGAGGAGACGAGAAAAGTGGGAGAAGAGGTCATCGAAAGAG
AACTCCAGAAGATGAATCCAAAGATCAGAGAGAGAGTGAGAGAGGGACTCGAAAAGATAA
AGCGCGGTGAGAGGGATGTCAGATTTTAATTAATTAACTTTAAGAAGGAGATATACATAC
CCGGGTAAGTTTAAACGGTC

Protein sequence predicted based on nucleotide sequence

MCMYVFVKERVESRSFIPEEKIFELLEKTKNPDPARVREIIQKSLDKNRLEPEETATLLN
VEDPELLEEIFEAARTLKERIYGNRIVLFAPLYIGNDCINDCVYCGFRVSNKVVERRTLT
EEQLKEEVKALVSQGHKRLIVVYGEHPNYSPEFIARTIDIVYNTKYGNGEIRRVNVNAAP
QTIEGYKIIKSVGIGTFQIFQETYHRETYLKLHPRGPKSNYNWRLYGLDRAMMAGIDDVG
IGALFGLYDWKFEVMGLLYHTIHLEERFGVGPHTISFPRIKPAINTPYSQKPEHVVSDED
FKKLVAIIRLSVPYTGMILTAREPAKLRDEVIKLGVSQIDAGSRIGIGAYSHKEDDEDRK
RQFTLEDPRPLDQVMRSLLKEGFVPSFCTACYRAGRTGEHFMEFAIPGFVKNFCTPNALF
TLQEYLCDYATEETRKVGEEVIERELQKMNPKIRERVREGLEKIKRGERDVRF*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Protein_Purified

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in 283132

Recommended expression in:

Not Applicable

Vector Name:

pMH2T7

Synonyms:

pMH-2-T7

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.

Comments:

None

Size (bp):

4084

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTAATTAATTAACTTTAAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColEI origin of replication

1921

2603

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

selectable marker

AmpR

ampicillin resistance gene

1164

1823

tag

His

N-terminal 6x His tag

367

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

Gene

Gene Symbol :

thiH

GENEID :

898216

Locus Tag :

TM1267

GI :

15644023

GenBank Accession :

AAD36342

Target GenBank:

NP_229072

Protein

SMART domain :

BATS : Biotin and Thiamin Synthesis associated domain

Structural Annotation Wiki :

TOPSAN

UniProt :

Q9X0Z4

Clone

Cloning Information :

276757

HIP Master Clone ID :

206243

Original Clone ID :

7662

Experimental

TargetTrack - Experimental data :

283132

Organism

Species Specific ID:

898216

Reagents

Labome :

thiH-cDNA