Gene:
Gene Symbol:
TM1552
Gene Name:
pyruvate formate-lyase activating enzyme
Original Clone ID:
20086
Keyword:
partial cds
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH2T7TEV
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00382519
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 984nts
Open reading frame: 51 to 1034
CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCGCTTTGCACT
TTGAAATTCTTGAGAATGGAAAAGTTAAGTGCCTTCTGTGTCCGCATGAATGTGTGCTTG
ATAACGGACAAATTGGGTTGTGCGGTGTGAGAAAGAACAAAAACGGCTCTCTGGTGAGTC
TGAACTACGGTGAAGTAACAGCAATCGCTATGGATCCCATAGAAAAGAAACCGCTTTTCC
ACTTCAATCCTGGTGAGCAGATATTCTCTGTGGGAACTTTCGGCTGTAATTTCAAGTGCG
GTTTCTGTCAGAACTGGGAGATTTCGCAAGCAAAGCCAGAAACCAAAAGAGTCACTCCAG
AACAGCTTGTCAAGATAGCCCAGATGAACAGAAACTCAAAAGGAATCGCTTTCACATACA
ACGAGCCTCTTGTCTGGTACGAGTTCGTTCTCGACACGTCTCGTGTGGCTGTGAGAGAAG
GTATGTACTGCGTTCTTGTGACCAACGGTTTTATAAACGAAGAACCTCTGGAACTCCTCT
TTCAGTCCGTGCATGCGATGAACATAGATCTCAAGGGATTCAACAGAGATTTCTACAGGG
AGATCGGGGGAGATTTGGACGTGGTCCTCAGAAACATAGAGAAGGTTTACAACGCAGGAA
TTCACGTTGAGCTCACCACGCTGATAATTCCGGGAAAGAACGACGACAAAGAAGACCTGA
GGAGAGAATTCGAGTGGATCGCTGATCTGGACAAAGACATCCCTCTTCATATAAGCCGCT
ACTTTCCAAATTACAAGTACACCATACCTCCAACGCCGATAGAAGAGCTCATCGAGATAT
ACGAGATGGCCAGAGAGTATTTAAATTTCGTCTACCTCGGTAACGTGTGGGACGAAAGAT
ACGAGAGCACTTTCTGCCCGGATTGCGGAAATCTTGTGATCAGAAGACAGGGATATGAAG
TGGAAAAAGTGGGACTGGATGAAGAAGGGAAGTGCACAAAATGTGGCCGTCAGATAGCAA
CGATCATCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGAT
TTTCA
Protein sequence predicted based on nucleotide sequence
ALHFEILENGKVKCLLCPHECVLDNGQIGLCGVRKNKNGSLVSLNYGEVTAIAMDPIEKK PLFHFNPGEQIFSVGTFGCNFKCGFCQNWEISQAKPETKRVTPEQLVKIAQMNRNSKGIA FTYNEPLVWYEFVLDTSRVAVREGMYCVLVTNGFINEEPLELLFQSVHAMNIDLKGFNRD FYREIGGDLDVVLRNIEKVYNAGIHVELTTLIIPGKNDDKEDLRREFEWIADLDKDIPLH ISRYFPNYKYTIPPTPIEELIEIYEMAREYLNFVYLGNVWDERYESTFCPDCGNLVIRRQ GYEVEKVGLDEEGKCTKCGRQIATIIG*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 360311
Recommended expression in:
Not Applicable
Vector Name:
pMH2T7TEV
Synonyms:
pMH-2-T7-TEV
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; amp resistance; PIPE cloning.
Comments:
None
Size (bp):
4105
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColEI origin of replication
1942
2624
inducible trxn activator protein gene
araC
araC gene
3199
4074
promoter
T7
T7 promoter
290
310
protease cleavage site
TEV
TEV protease cleavage site
382
402
selectable marker
AmpR
ampicillin resistance gene
1185
1844
tag
His
N-terminal 6xHis tag
364
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
trxn termination sequence
rrnB
rrnB T2 transcription termination
849
875
