Dde_1616 (O. alaskensis G20 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Dde_1616
Gene Name:  metallo-beta-lactamase
Original Clone ID:
24828
Keyword:
full-length cds
Species:
Oleidesulfovibrio alaskensis G20
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
DdCD00382661
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1158nts
Open reading frame: 51 to 1208

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGAAAGAAA
ACAATATGAGCCGGCGCGACTTTGTGAAAGGTGTGGCCACCGGTGTCTGCCCTGGTGCCT
TTGCCTCCATGGGCCTTTATTCTTATACCGGTGCGGCCTACGACAGACTGCCCAAGTCGG
AAAGAAAGTTTCATGATTTCGGTGCTGTGCGCAGCGTGAAAGTTGTGAACATTTCCGAGA
CAAGCTGGTTCAGCAATGCTGACCTGATCGGAGACATTCATGCTGCCGGCGGCCTGCTGG
TAAACCAGTACACGCTTAACTGGGCACCTTTTGCCAATGGTAAAGGCACTGCAAAGGGTT
CGTATGATTCCGGTCTGGCAAGCATCAAAGATCTTATTCCCCATGATCTGGAAAAAGCGT
GGGATATCCAGCGCAAGCTGGCTCTGCACCCTGAAAACCCCGGCGGTTTTTCATGCCTGC
TGGAAGTGGAAGCTCTGGACGGCACAAAGCATAATTACCTGCTGGACAGCGGCTGGTCCT
ACGAATGGATGGATAAGAGCTTCAAGCGGGAAGGCATCGACAAGATGCTTGAGGCCCGGC
AGATTGAGGGGCTGTTCATTTCGCATGAACACTGGGATCATTTCTGGGGACTGCCCGTCA
CCATGAAGTATGACAACCGTATTCCGCTGTACATACACGACGGCTTTTACAAAGAAGGGC
TGCAGTACATAAAGGACAGCGGGTACAAGGGCACACCTTTCATACAGAAGGAACCCTTGC
ATCAGGTCATGCCGGGCATGGCAGTGGTGAAGTTCGATGTGCCCATCATCAACCGCGTGT
TCGGCGAAACGTCTCTGGCCTTCAATGTGAAGGACAAAGGCCTTGTGCTCATCTCGGGTT
GCTGCCATCAGGGCATTCTGCAGATGGCCGACTTGGCCTATACCGACCTTAAATACGATA
ATGACAAGTTCTCCGGCATCTACGGCGGGCTGCACATATCGCCCTTCGAAGACTGGGATC
CCAAGTACGACGATCTGGTGATCTCGCTGGGCAAGTGGAACTTCGACCGCATCGGCTGCA
ACCATTGCACCGGCCACCTGACCGCCAGAAAGTTCATAGAGCGCGGCTATCCCGTGGTAC
GCGGGTCGGCGCGGTTCCGTTGGTCTTCGCCTGATTATCTGGGCAACGGCGATACCATTA
CCTTCTAACGCGACTTAATTAACTCGTTTAAACGGTCTCCAGC

Protein sequence predicted based on nucleotide sequence

MKENNMSRRDFVKGVATGVCPGAFASMGLYSYTGAAYDRLPKSERKFHDFGAVRSVKVVN
ISETSWFSNADLIGDIHAAGGLLVNQYTLNWAPFANGKGTAKGSYDSGLASIKDLIPHDL
EKAWDIQRKLALHPENPGGFSCLLEVEALDGTKHNYLLDSGWSYEWMDKSFKREGIDKML
EARQIEGLFISHEHWDHFWGLPVTMKYDNRIPLYIHDGFYKEGLQYIKDSGYKGTPFIQK
EPLHQVMPGMAVVKFDVPIINRVFGETSLAFNVKDKGLVLISGCCHQGILQMADLAYTDL
KYDNDKFSGIYGGLHISPFEDWDPKYDDLVISLGKWNFDRIGCNHCTGHLTARKFIERGY
PVVRGSARFRWSSPDYLGNGDTITF*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g61c,A21P;t885g,F295L;
a923c,Y308S;c1112g,S371W
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 367917
Recommended expression in:
Not Applicable