NCgl1859 (C. glutamicum )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  NCgl1859
Gene Name:  transcriptional regulator of sugar metabolism
Original Clone ID:
25322
Keyword:
full-length cds
Species:
Corynebacterium glutamicum
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
CgCD00382672
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 795nts
Open reading frame: 51 to 845

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGGTCAGCC
AAACGGAAAGACAGCATGCAATTGCTTCTTTACTGGCACCAACTGGTGCGGTGTCTGTAG
GAGATTTAGCCGAGCATTTCCATGTGACAACCGAAACAGTGCGACGTGATCTTCGGATCA
TGGAGTCACTGGGTTTGTTGCAACGAGTTCACGGTGGCGCCATTAGCCCAGAGCCCATGG
GTACGTCTCCCCCTCGGCTGAAACCTGCCTTGGTTAAAGGAATGCCACCGGAACCCCGTG
TTTTAGAACTTGCAGAAACTGCAGTTTCCCTCATCACACCTCTAGCACGCAGCATTTTCC
TGGATTCAGGTTTAGCGTGCACGGCGATTGCCACGGTGTTGGGGGATCCTCCAGAAGATG
CCAGGTGGACTGTTGTTACAAGTTCCCCCGGCGCTGTGATTGCCTTGTCCGCGACAGATG
CCACCTCCACGGTGGTGCTGCACGGGCAGGTTCACGGTAATTGTTCTTCAATCATTGGGT
CCACGGCAGTAGACATGATTTCGCAGTTGCGCGCTGATATCGCCTTCGTGGAGGTTGATG
TGATTCAATCCGATACAAGTCTGTGCACGTTTTTCCCGGAGACGATTCCCATCAAGCAAG
CCATGATCAAAAACGCGGCTTTCACAGTTGCTGTTCTCAGCCCGAGATCTCCCCAAGATC
AAGAACTTCAACTTTTGAAGCACCCTTTTTCCACCTTGGCTGATTTTGATGCCCTTGTTA
CCGATGACCACACGCTAGATTTTCCAGTTTTGCCCGACCACAACTTTCAGGTGGTAACCC
CATAACGCGACTTAATTACTCGTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGAT

Protein sequence predicted based on nucleotide sequence

MVSQTERQHAIASLLAPTGAVSVGDLAEHFHVTTETVRRDLRIMESLGLLQRVHGGAISP
EPMGTSPPRLKPALVKGMPPEPRVLELAETAVSLITPLARSIFLDSGLACTAIATVLGDP
PEDARWTVVTSSPGAVIALSATDATSTVVLHGQVHGNCSSIIGSTAVDMISQLRADIAFV
EVDVIQSDTSLCTFFPETIPIKQAMIKNAAFTVAVLSPRSPQDQELQLLKHPFSTLADFD
ALVTDDHTLDFPVLPDHNFQVVTP*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g224t,G75V;c551t,A184V
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 367121
Recommended expression in:
Not Applicable