Nmul_A0230 (N. multiformis ATCC 25196 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Nmul_A0230
Gene Name:  NAD-dependent epimerase/dehydratase
Original Clone ID:
36519
Keyword:
full-length cds
Species:
Nitrosospira multiformis ATCC 25196
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
NmCD00382701
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 915nts
Open reading frame: 51 to 965

GATCTGATAAAATTCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGTCGGAGT
CCTGGGTTGCCGTTACCGGCGCCACTGGCTTTATCGGAAATGCTCTGCTGCAGTCCCTGA
TAAAGGAAGGATGGAAAGTCAGGGCTCTGACAAGACGTGCGCGCGTCGATGACGAGTCCA
CGCAATGGATAGAAGGCGATTTACATGACCGCGATGCATTGCGAAAACTCGTAACAGGTG
TCTCGGCCGTTGTGCATTGCGCAGGTCAGGTTCGGGGAAGCTCTCTCGAGAGCTTTGTCC
GTACCAATGTGGAAGGCACGGCCAACCTGATGCAGGCTTCTCTGGAACAAAACCCGCCTC
CACGTTTCCTGCTGGTTTCTTCTCTGGCCGCCCGGCAACCCGAGCTTTCCTGGTACGCCA
CGAGCAAGCACATGGCTGAACGTCTGATAGACGAGCGCTCGGCGGGTATGGCCTGTGCCA
TTTTTCGTCCAACGGCTGTTTATGGTCCCGGAGATAAGGAAATGAGCCCGCTGTTCAGGA
TAACGCGCCGCGGTATCTTGCCAATGGTGGGGCAACCTGAGATGCGGTTTGGACTACTGC
ATGTCGCCGATCTGGTAGCGGCAATCACCTGCTGGCTTTCGACTGCGGTGCCTGTACAAG
GTACCTACGAGCTTGATGACGGTATGCCGGGAGGTTACGACAGCCAGGCGGTAGCCACTA
TTGCACAGGAAGTGTGGAAACGCCCTGTACGCTGCGTCTTTTTCCCCGCACCACTGGTTT
CATTGATCGCAAATATCAACTTGTGGTCTGCCAGAATGCTAAGCTATTCACCTATGCTGA
CCCCGGGAAAAGTGAGAGAATTGCGCCACCCCGACTGGGTTTGTGATATTGATCCCCTGA
CTCGGGCACTCCCAGGGTGGCGACCCTGCATCCACCTTCGCGACGCTTTACCACAAGCAG
TCTAACGCGACTTAATTAA

Protein sequence predicted based on nucleotide sequence

MSESWVAVTGATGFIGNALLQSLIKEGWKVRALTRRARVDDESTQWIEGDLHDRDALRKL
VTGVSAVVHCAGQVRGSSLESFVRTNVEGTANLMQASLEQNPPPRFLLVSSLAARQPELS
WYATSKHMAERLIDERSAGMACAIFRPTAVYGPGDKEMSPLFRITRRGILPMVGQPEMRF
GLLHVADLVAAITCWLSTAVPVQGTYELDDGMPGGYDSQAVATIAQEVWKRPVRCVFFPA
PLVSLIANINLWSARMLSYSPMLTPGKVRELRHPDWVCDIDPLTRALPGWRPCIHLRDAL
PQAV*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 380771
Recommended expression in:
Not Applicable