Dde_1741 (O. alaskensis G20 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Dde_1741
Gene Name:  prophage antirepressor
Original Clone ID:
36232
Keyword:
full-length cds
Species:
Oleidesulfovibrio alaskensis G20
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
DdCD00382725
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 555nts
Open reading frame: 51 to 605

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGGGTGAAA
TCAGCACACTCACCCTCAACGGCATGGCTGATGCCCGAACCATCGTGGATGAAAACGGTG
AACTCTGGTTCGTCGCAATGGATGTTTGCAAGCACCTAGGTCTCAAGCCGAGGGATTCAG
TGCGCTACTTGGACGACGACATGAAAAAGCATCTTCCAAGAACCGCCCTCGGAATGAAGC
CCGGAAAGCCGCTGCTGATTATCAACGAACCCGGTCTGTACACGCTCATATTCCAAAGCC
GAAAGCCTGAAGCCATCGCGTTTCAGGATTGGGTTTGCAAGGAAGTCCTGCCCTCGATCC
GGAAGCATGGTGCGTACTTCATGATGAAGCCCACGGATACGGATGAAAGCATAATCCAGA
AGGCAACGCAGATCATTGCACTGGCGCGTGAAGATGACGGCATTGAATGCCCCGACATCT
TGCGAGAGTTGGCAGACAAGCTTGAAGCCGCCCTGCAAGGTCAATTCATGCCGACATTCA
CGACTAGCACTTGCAAGGCTTATTCGAATGACACCAGCGACTTAGGCCCAATTATTGACG
TCTAACGCGACTTAATTAATTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGA

Protein sequence predicted based on nucleotide sequence

MGEISTLTLNGMADARTIVDENGELWFVAMDVCKHLGLKPRDSVRYLDDDMKKHLPRTAL
GMKPGKPLLIINEPGLYTLIFQSRKPEAIAFQDWVCKEVLPSIRKHGAYFMMKPTDTDES
IIQKATQIIALAREDDGIECPDILRELADKLEAALQGQFMPTFTTSTCKAYSNDTSDLGP
IIDV*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g267c,M89I;g289a,E97K;
t303c,P101P;t342c,P114P;
g357a,E119E;t377c,M126T;
a389c,E130A;a465g,L155L;
t491c,M164T;g493a,c495t,A165T
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 381688
Recommended expression in:
Not Applicable