SMU.1388 (S. mutans ) in pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  SMU_1388
Gene Name:  RNA helicase
Original Clone ID:
36029
Keyword:
full-length cds
Species:
Streptococcus mutans
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
SmCD00382756
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1083nts
Open reading frame: 51 to 1133

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGAAAACAC
AATTTCCAAGCGTTTGGCAAGATAAACTCAGCCAAAAACATTTTTCAGCATTTACGACCA
TTCAAGAAGCTGCTTTTGACCCTATTAAAAATGGTAAGAGTCTTCTTGGTATCAGCCCCA
CTGGTAGTGGTAAGACCTTAGCCTATCTTTGGCCTAGTTTACTCACTCTCACACCTAAAA
AAGCTCAACAATTGCTCATTCTAGCTCCTAATACCGAGTTAGCTGGACAGATCTTTGAAG
TTACCAAGGAATGGGCTCAACCTCTTAATTTAACAGCTCAGCTTTTTTTATCAGGTTCCA
GTCAAAAACGACAAATTGAACGTTTAAAAAAAGGACCAGAAATTATCATTGGCACATCTG
GGCGTGTCTTTGACCTTGTGAAACTCAAAAAGATTAAGATGATGAACGTTAATACAATTG
TTCTAGATGAATTTGATGACTTACTTGGTAAATCTCAAATTCACTTTGTTGAAAATATTG
TGAAACGTGTTCCTCGTGACCATCAGATGATTTATATGAGTGCAACAAATAGCATTGATA
GAACATTTTTGACTGATGATACCCTTGAGATTGACTTATCAAAACAAACGGTAGACACCA
TTTCTCATTATTATATCACTGTTGCTAAGCGTGAACGTGGAGAATTGCTGCGTAAATTTT
CTAACATTCCTAACTTTAGGGGACTTGTTTTCTTTAACAGGCTGTCAGACTTAGGAGCCA
ATGAAGAGCGTCTACAATTTAGCGGTGCTTCTGCTGTTTCACTTGCCAGTGATGTTAATA
TTAAATTCCGCAAAGTCATTTTAGAAAAATTCAAAAATCATGAACTCTCTCTATTGCTGG
CAACAGATTTAGTAGCGCGCGGCATTGATATTGAGCTTCTCGAAACAGTTGTCAACTTTG
ATTTACCAAGAGATAAAGATGCATACAATCACCGTGCCGGACGAACAGGACGCATGGGAA
AAAATGGTATTGTTGTTACCTTTATTAGTCATCCTGAAGAACTTAAAAAACTGAAAAAAT
TTGCACACGTTTCAGAAGTCTATCTTAAAAATCAGATTTTACATAAAAAATAACGCGACT
TAATTAATTTGGCGGATGAGAGAAGATTTTCA

Protein sequence predicted based on nucleotide sequence

MKTQFPSVWQDKLSQKHFSAFTTIQEAAFDPIKNGKSLLGISPTGSGKTLAYLWPSLLTL
TPKKAQQLLILAPNTELAGQIFEVTKEWAQPLNLTAQLFLSGSSQKRQIERLKKGPEIII
GTSGRVFDLVKLKKIKMMNVNTIVLDEFDDLLGKSQIHFVENIVKRVPRDHQMIYMSATN
SIDRTFLTDDTLEIDLSKQTVDTISHYYITVAKRERGELLRKFSNIPNFRGLVFFNRLSD
LGANEERLQFSGASAVSLASDVNIKFRKVILEKFKNHELSLLLATDLVARGIDIELLETV
VNFDLPRDKDAYNHRAGRTGRMGKNGIVVTFISHPEELKKLKKFAHVSEVYLKNQILHKK
*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t243c,I81I;g253a,A85T;
c288t,A96A;c367t,P123S;
a372g,G124G;t378c,V126V;
a481g,K161E;g562a,A188T;
c648t,R216R;a901g,I301V
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 378716
Recommended expression in:
Not Applicable