NMA0028 (N. meningitidis )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  NMA0028
Gene Name:  hypothetical protein
Original Clone ID:
25937
Keyword:
partial cds
Species:
Neisseria meningitidis
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
NpCD00383017
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 375nts
Open reading frame: 51 to 425

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCTACGGCGGCG
AATACGGCCCGGATTTGGCGGAAGTCGCTGCTTTCCATCAGACGGTTATTTCCGAAATCG
TCCGCCGCCATACGGCGCAAACTTATACCGTATTTATGATGGGCTTCCAGCCTGGTTTCC
CTTATCTGGGCGGCTTGCCCGAAGCATTGCACACGCCCCGCCGTGCCGTGCCGAGAACGT
CCGTTCCTGCCGGTTCGGTCGGTATCGGCGGCAGTCAGACCGGTGTGTATCCGTTCGCTT
CGCCCGGCGGCTGGCAGATTATCGGCAGAACCGAATTACCCTTGTTCCGAGCCGATTTGA
ATCCGCCGACCCTGCTGGCGGCGGGTGACCAAGTCCGCTTTGTTGCAGAAAGGATTGAGC
CATAACGCGACTTAATTAACTCGTTTA

Protein sequence predicted based on nucleotide sequence

YGGEYGPDLAEVAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAV
PRTSVPAGSVGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAE
RIEP*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g12a,E4E;c21g,P7P;
c24t,D8D;c25t,L9L;
c30g,A10A;g33a,E11E;
g36c,V12V;c39t,A13A;
c42t,A14A;c48t,H16H;
c54g,T18T;g57t,V19V;
c60t,I20I;a61t,g62c,S21S;
g66a,E22E;g72c,V24V;
a73c,a75c,R25R;a76c,a78c,R26R;
c81t,H27H;c84g,T28T;
c87g,A29A;g90a,Q30Q;
c93t,T31T;c96t,Y32Y;
g102a,V34V;c105t,F35F;
c123t,P41P;c126t,G42G;
c132t,P44P;c135t,Y45Y;
c145t,L49L;g153a,E51E;
c156a,A52A
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 370771
Recommended expression in:
Not Applicable