Gene:
Gene Symbol:
ECs0699
Gene Name:
(dimethylallyl)adenosine tRNA methylthiotransferase
Original Clone ID:
726a1TNt2b1
Keyword:
full-length cds
Species:
Escherichia coli
Type:
cDNA
Vector Name:
pSB2
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
EcCD00383237
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1434nts
Open reading frame: 16 to 1449
GGTCATATGTCCCTTACCAAAAAACTCCATATTAAAACCTGGGGCTGTCAGATGAACGAG
TACGATTCATCGAAGATGGCCGATCTGCTGGATGCCACCCACGGCTATCAACTGACCGAC
GTGGCGGAAGAAGCGGATGTGCTGCTGCTGAACACCTGCTCAATCCGCGAGAAGGCTCAG
GAAAAAGCCTTCCATCAGTTGGGTCGCTGGAAACTGTTAAAAGAGAAGAATCCAGACCTG
ATTATCGGCGTCGGTGGCTGCGTGGCATCGCAAGAAGGCGAGCACATTCGCCAGCGCGCC
CACTATGTCGATATTATTTTTGGGCCGCAAACGCTGCACCGTCTGCCGGAGATGATCAAC
TCCGTGCGTGGCGACCGCAGCCCGGTTGTAGATATCAGCTTCCCGGAAATCGAGAAGTTT
GACCGTCTACCGGAACCGCGCGCCGAAGGGCCGACCGCGTTTGTCTCCATCATGGAAGGC
TGCAATAAATATTGCACCTACTGCGTGGTGCCTTACACCCGTGGTGAAGAGGTAAGCCGT
CCGTCCGACGATATTCTGTTTGAGATTGCCCAGCTTGCGGCTCAGGGCGTGCGTGAAGTC
AACCTGCTCGGTCAGAACGTGAACGCCTGGCGTGGTGAAAACTACGACGGCACCACCGGA
TCGTTTGCCGATCTGCTGCGTCTGGTTGCTGCGATCGACGGGATCGATCGTATTCGCTTT
ACCACCAGCCATCCGATCGAATTCACCGACGATATCATCGAAGTGTATCGCGACACGCCG
GAGCTGGTGAGCTTCCTGCATCTGCCGGTACAGAGCGGTTCCGATCGCATTCTGAACCTG
ATGGGCCGTACCCATACGGCGCTGGAGTACAAAGCGATCATCCGTAAACTGCGTGCGGCG
CGTCCAGATATTCAGATCAGTTCTGATTTCATCGTTGGCTTCCCTGGCGAAACCACCGAA
GACTTCGAGAAAACGATGAAGCTGATTGCCGACGTCAATTTCGACATGAGCTACAGCTTT
ATCTTCTCTGCACGTCCGGGTACACCAGCCGCCGATATGGTTGATGATGTTCCGGAAGAA
GAGAAGAAGCAGCGTCTGTATATTCTGCAAGAGCGCATTAATCAGCAAGCGATGGCGTGG
AGCCGCCGGATGCTCGGCACCACCCAGCGTATTCTGGTAGAAGGTACATCGCGTAAGAGC
ATCATGGAGCTTTCCGGGCGTACGGAAAATAACCGCGTGGTCAACTTCGAAGGCACGCCG
GATATGATCGGTAAATTCGTCGATGTAGAAATTACCGACGTCTACCCGAACTCTCTGCGC
GGTAAAGTGGTGCGTACTGAAGATGAAATGGGTCTGCGCGTGGCAGAAACACCGGAATCA
GTGATTGCCCGTACCCGCAAAGAAAACGACCTTGGCGTGGGTTATTATCAGCCGGAAGGG
GGATCCTAAACTGCAGAGATCCGAATTCGAGCTCCGTCG
Protein sequence predicted based on nucleotide sequence
TKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKAFH QLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGD RSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR LYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMIGK FVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQPEGGS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t173c,V58A
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in NYSGXRC-726a
Recommended expression in:
Not Applicable
Vector Name:
pSB2
Synonyms:
pSB-2, pSB2_TN
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds N-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
TOPO modified pSB2 vector -by Invitrogen. Stop codon generated by adding an extra G to the reverse primer which puts the insert in-frame with vector-encoded stop when cloned into the TA site.
Size (bp):
5338
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
TOPO recognition site
TOPO
TOPO cloning site
5110
5119
bacterial origin
ori
ColE1 bacterial origin of replication
1420
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4984
5003
repressor protein gene
LacI
LacI cds
3770
4594
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5079
5096
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5267
5313
