yhbQ (E. coli ) in pSB3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  yhbQ
Gene Name:  conserved protein, GIY-YIG nuclease superfamily
Original Clone ID:
3451a1TCt1b1
Keyword:
full-length cds
Species:
Escherichia coli
Type:
cDNA
Vector Name:
pSB3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified. Actual 5' flanking sequence provided below.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
EcCD00383257
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 330nts
Open reading frame: 51 to 380

TTCCTCTGAATATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTACACCCCGGT
TTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCG
AACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAG
AACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAAT
ATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGT
TTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATGAAGGGGGATCCC
ACCACCACCACCACCACTGAGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCAC
TCGAGCACCAC

Protein sequence predicted based on nucleotide sequence

TPRFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKGELTLVFSAPVGDRSLAL
RAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSDEGGSHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t7c,W3R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in NYSGXRC-3451a
Recommended expression in:
Not Applicable