bioB (H. halodurans )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  bioB
Gene Name:  biotin synthase
Original Clone ID:
6215b1BCt7b1
Keyword:
full-length cds
Species:
Halalkalibacterium halodurans
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified. Actual 5' flanking sequence provided below.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
BhCD00383285
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1020nts
Open reading frame: 42 to 1061

AATTTTGTTTAACTTTAGAAGGAGATATACATATGTCCCTTAATTGGATTCAGCTTGCCC
AAGAAGTGATTGAGGGGAAACTTATATCAGAAAATGAAGCGTTAGCCATTCTTAACAGCC
CTGACGACGAACTTTTGCTTTTGTTGCAAGGGGCGTTCACGATCCGGCAAACATACTACG
GAAAGAAAGTAAAATTAAATATGATTATGAACGCAAAATCAGGATTTTGTCCAGAGAATT
GCGGTTATTGTTCCCAGTCGTCCATATCGAAGGCACCGATTGACGCGTACCCGATGGTGA
ATAAAGAAACCATTTTGGAAGGCGCAAAACGGGCTCATGAATTAAATGTGGGGACGTACT
GTATTGTGGCCAGTGGGCGTGGACCTACGAATCGAGACATTGATCATGTTACGGAAGCGG
TTCGCGAAATTAAAGATACGTATGGATTAAAGATATGTGCTTGTCTCGGTATTTTAAAAC
CGGAGCAGGCTGAGCAGTTAAAAGCGGCAGGGGTTGACCGATATAATCACAATGTTAATA
CATCGGCTCGCCACCATGATCAGATCACGACCTCACACACGTATGAGGATCGGGTAAACA
CGGTCGAAGTTGTAAAGCATAGCGGGATTTCACCGTGCTCAGGTGTGATCGTTGGTATGA
AAGAAACAAAGGAAGATGTTGTGGACATGGCCTTTCAATTGCGGGAACTCGATGCCGATT
CCATTCCGGTGAATTTCTTACATGCGATAGACGGGACACCGCTTCAAGGCGTCCATGAAC
TGACTCCTATTTATTGCTTGAAAGTATTATCCTTATTCCGCTATGTATGTCCAACGAAGG
AAATACGGATCTCAGGGGGAAGGGAAGTCAATTTAAAGAGCCTTCAACCACTTGGCCTTT
ATGCAGCTAACTCCATTTTCATCGGTGATTATTTGACGACAGCAGGTCAGGAGGAAACGG
CTGACCATCAAATCCTGAAAGATTTAGGATTCGAAGTGGAATCTGTGGAAGAAATGAAAG
CAAGCCTGCAAGGCCAAGGGCATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTC
GACAAGCTT

Protein sequence predicted based on nucleotide sequence

NWIQLAQEVIEGKLISENEALAILNSPDDELLLLLQGAFTIRQTYYGKKVKLNMIMNAKS
GFCPENCGYCSQSSISKAPIDAYPMVNKETILEGAKRAHELNVGTYCIVASGRGPTNRDI
DHVTEAVREIKDTYGLKICACLGILKPEQAEQLKAAGVDRYNHNVNTSARHHDQITTSHT
YEDRVNTVEVVKHSGISPCSGVIVGMKETKEDVVDMAFQLRELDADSIPVNFLHAIDGTP
LQGVHELTPIYCLKVLSLFRYVCPTKEIRISGGREVNLKSLQPLGLYAANSIFIGDYLTT
AGQEETADHQILKDLGFEVESVEEMKASLQGQGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g41t,R14L;del@995,3
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-6215b
Recommended expression in:
Not Applicable