DNASU Plasmid | PTPN18 (H. sapiens )<br>In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: PTPN18

Gene Name: protein tyrosine phosphatase, non-receptor type 18 (brain-derived)

Original Clone ID:

8631a2BCt2p1

Keyword:

full-length cds

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pSGX3

Format:

CLOSED

Source:

New York SGX Research Center for Structural Genomics

Description:

None

Comments:

Linker not verified. Actual 5' flanking sequence provided below.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
New York SGX Research Center for Structural Genomics

HsCD00383311

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 924nts

Open reading frame: 39 to 962

Reference Sequence Alignment

TTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTAGCCGCAGCCTGGACTCGGCGC
GGAGCTTCCTGGAGCGGCTGGAAGCGCGGGGCGGCCGGGAGGGGGCAGTCCTCGCCGGCG
AGTTCAGCGACATCCAGGCCTGCTCGGCCGCCTGGAAGGCTGACGGCGTGTGCTCCACCG
TGGCCGGCAGTCGGCCAGAGAACGTGAGGAAGAACCGCTACAAAGACGTGCTGCCTTATG
ATCAGACGCGAGTAATCCTCTCCCTGCTCCAGGAAGAGGGACACAGCGACTACATTAATG
GCAACTTCATCCGGGGCGTGGATGGAAGCCTGGCCTACATTGCCACGCAAGGACCCTTGC
CTCACACCCTGCTAGACTTCTGGAGACTGGTCTGGGAGTTTGGGGTCAAGGTGATCCTGA
TGGCCTGTCGAGAGATAGAGAATGGGCGGAAAAGGTGTGAGCGGTACTGGGCCCAGGAGC
AGGAGCCACTGCAGACTGGGCTTTTCTGCATCACTCTGATAAAGGAGAAGTGGCTGAATG
AGGACATCATGCTCAGGACCCTCAAGGTCACATTCCAGAAGGAGTCCCGTTCTGTGTACC
AGCTACAGTATATGTCCTGGCCAGACCGTGGGGTCCCCAGCAGTCCTGACCACATGCTCG
CCATGGTGGAGGAAGCCCGTCGCCTCCAGGGATCTGGCCCTGAACCCCTCTGTGTCCACT
GCAGTGCGGGTTGTGGGCGAACAGGCGTCCTGTGCACCGTGGATTATGTGAGGCAGCTGC
TCCTGACCCAGATGATCCCACCTGACTTCAGTCTCTTTGATGTGGTCCTTAAGATGAGGA
AGCAGCGGCCTGCGGCCGTGCAGACAGAGGAGCAGTACAGGTTCCTGTACCACACGGTGG
CTCAGATGTTCTGCTCCACACTCCAGAATGCCAGCGAAGGGCATCATCACCATCACCACT
GATCCGAATTCGAGCTCCGTCGACA

Protein sequence predicted based on nucleotide sequence

SRSLDSARSFLERLEARGGREGAVLAGEFSDIQACSAAWKADGVCSTVAGSRPENVRKNR
YKDVLPYDQTRVILSLLQEEGHSDYINGNFIRGVDGSLAYIATQGPLPHTLLDFWRLVWE
FGVKVILMACREIENGRKRCERYWAQEQEPLQTGLFCITLIKEKWLNEDIMLRTLKVTFQ
KESRSVYQLQYMSWPDRGVPSSPDHMLAMVEEARRLQGSGPEPLCVHCSAGCGRTGVLCT
VDYVRQLLLTQMIPPDFSLFDVVLKMRKQRPAAVQTEEQYRFLYHTVAQMFCSTLQNASE
GHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

ProteinPurified: Tested_Not_Purified

Find experimental protocols in NYSGXRC-8631a

Recommended expression in:

Not Applicable

Vector Name:

pSGX3

Synonyms:

pSGX-3, pSGX3_BC

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.

Comments:

Modified from pET26b from Novagen

Size (bp):

5339

Parent Vector:

None

Properties:

TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker

Author Name:

New York SGX Research Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCTTAAGGGCATCATCACCATCACCACTGATCCCACCACCACCACCACCACTGAGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAT

Vector Features:

Type

Name

Description

Start

End

ATG start

start

initiation start (Met, Ser, Leu)

5072

5079

TOPO recognition site

TOPO

TOPO cloning site

5076

5085

bacterial origin

ori

pBR322-type bacterial origin of replication

1421

2103

gene fragment

ROP

2527

2703

promoter

T7 promoter

4983

5003

repressor protein gene

LacI

LacI CDS

3515

4531

selectable marker

KanR

kanamycin resistance gene

563

1375

tag

His

6xHis tag

5086

5103

trxn regulatory element

lac operator

5003

5025

trxn termination sequence

T7 term

T7 terminator

5231

5307

Gene

Gene Symbol :

PTPN18

Symbol Nomenclature :

PTPN18

SYNONYM :

BDP1; PTP-HSCF

Designation :

brain-derived phosphatase|tyrosine-protein phosphatase non-receptor type 18

Full Nomenclature :

protein tyrosine phosphatase, non-receptor type 18 (brain-derived)

GENEID :

26469

GI :

18375655

GenBank Accession :

NM_014369

HGNC :

9649

MIM :

606587

Vega :

OTTHUMG00000131630

Target GenBank:

10795928

Reference Sequence Alignment

  

HsCD00383311        TTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTAGCCGCAGCCTGGACTCGGCGC
NM_014369.3         -----------------------------------ATGAGCCGCAGCCTGGACTCGGCGC
NM_001142370.1      -----------------------------------ATGAGCCGCAGCCTGGACTCGGCGC
                                                       .* **********************

HsCD00383311        GGAGCTTCCTGGAGCGGCTGGAAGCGCGGGGCGGCCGGGAGGGGGCAGTCCTCGCCGGCG
NM_014369.3         GGAGCTTCCTGGAGCGGCTGGAAGCGCGGGGCGGCCGGGAGGGGGCAGTCCTCGCCGGCG
NM_001142370.1      GGAGCTTCCTGGAGCGGCTGGAAGCGCGGGGCGGCCGGGAGGGGGCAGTCCTCGCCGGCG
                    ************************************************************

HsCD00383311        AGTTCAGCGACATCCAG-------------------------------------------
NM_014369.3         AGTTCAGCGACATCCAGGCCTGCTCGGCCGCCTGGAAGGCTGACGGCGTGTGCTCCACCG
NM_001142370.1      AGTTCAGCAAAAGGTG--------------------------------------------
                    ********.*.*   .                                            

HsCD00383311        ------------------------------------------------------------
NM_014369.3         TGGCCGGCAGTCGGCCAGAGAACGTGAGGAAGAACCGCTACAAAGACGTGCTGCCTTATG
NM_001142370.1      ------------------------------------------------------------
                                                                                

HsCD00383311        ------------------------------------------------------------
NM_014369.3         ATCAGACGCGAGTAATCCTCTCCCTGCTCCAGGAAGAGGGACACAGCGACTACATTAATG
NM_001142370.1      ------------------------------------------------------------
                                                                                

HsCD00383311        ------------------------------------------------------------
NM_014369.3         GCAACTTCATCCGGGGCGTGGATGGAAGCCTGGCCTACATTGCCACGCAAGGACCCTTGC
NM_001142370.1      ------------------------------------------------------------
                                                                                

HsCD00383311        ------------------------------------------------------------
NM_014369.3         CTCACACCCTGCTAGACTTCTGGAGACTGGTCTGGGAGTTTGGGGTCAAGGTGATCCTGA
NM_001142370.1      ------------------------------------------------------------
                                                                                

HsCD00383311        ------------------------------------------------------------
NM_014369.3         TGGCCTGTCGAGAGATAGAGAATGGGCGGAAAAGGTGTGAGCGGTACTGGGCCCAGGAGC
NM_001142370.1      -------------------------------------TGAGCGGTACTGGGCCCAGGAGC
                                                                                

HsCD00383311        --------------------------GCCTGCTCGGCCGCCTGGAAGGCTGACGGCGTGT
NM_014369.3         AGGAGCCACTGCAGACTGGGCTTTTCTGCATCACTCTGATAAAGGAGAAGTGGCTGAATG
NM_001142370.1      AGGAGCCACTGCAGACTGGGCTTTTCTGCATCACTCTGATAAAGGAGAAGTGGCTGAATG
                                                *: *:*    . .:.*.**..  .    .:  

HsCD00383311        GCTCCACCGTGGCCGGCAGTCGGCCAGAGAACGTGAGGAAGAACCGCTACAAAGACGTGC
NM_014369.3         AGGACATCATGCTCAGGACCCTCAAGGTCACATTCCAGAAGGAGTCCCGTTCTGTGTACC
NM_001142370.1      AGGACATCATGCTCAGGACCCTCAAGGTCACATTCCAGAAGGAGTCCCGTTCTGTGTACC
                    .  .** *.**  *.* *  *  ...*: *.. * ..****.*   * . :.:*:  : *

HsCD00383311        TGCCTTATGATCAGACGCGAGTAATCCTCTCCCTGCTCCAGGAAGAGGGACACAGCGACT
NM_014369.3         AGCTACAGTATATGTCCTGGCCAGACCGTGGGGTCCCCAGCAGTCCTGACCACATGCTCG
NM_001142370.1      AGCTACAGTATATGTCCTGGCCAGACCGTGGGGTCCCCAGCAGTCCTGACCACATGCTCG
                    :** : *  **.:*:*  *.  *.:**      * * *.. ..: . *..****   :* 

HsCD00383311        ACATTAATGGCAACTTCATCCGGGGCGTGGATGGAAGCCTG------------GCCTACA
NM_014369.3         CCATGGTGGAGGAAGCCCGTCGCCTCCAGGGATCTGGCCCTGAACCCCTCTGTGTCCACT
NM_001142370.1      CCATGGTGGAGGAAGCCCGTCGCCTCCAGGGATCTGGCCCTGAACCCCTCTGTGTCCACT
                    .*** .: *. .*.  *.  **   * :**.:  :.***              * * **:

HsCD00383311        TTGCCACGCAAGGACCCTTGCCTCACACCCTGCTAGACTTCTGGAGACTGGTCTGGGAGT
NM_014369.3         GCAGTGCGGGTTGTGGGCGAACAGGCGTCCTGTGCACCGTGGATTATGTGAGGCAGCTGC
NM_001142370.1      GCAGTGCGGGTTGTGGGCGAACAGGCGTCCTGTGCACCGTGGATTATGTGAGGCAGCTGC
                      .  .** .: *:     ..*: .*. ****  ...* *  . :.: **.   .* :* 

HsCD00383311        TTGGGGTCAAGGTGATCCTGATGGCCTGTCGAGAGATAGAGAATGGGCGGAAAAGGTGTG
NM_014369.3         TCCTGACCCAGATGATCCCACCTGACTTCAGTCTCTTTGATGTGGTCCTTAAGATGAGGA
NM_001142370.1      TCCTGACCCAGATGATCCCACCTGACTTCAGTCTCTTTGATGTGGTCCTTAAGATGAGGA
                    *   *. *.**.****** ..  *.**  .*: : :*:** .: *  *  **.* *:* .

HsCD00383311        AGCG------------------------GTACTGGGCCCAGGAGCAGGAGCCACTGCAGA
NM_014369.3         AGCAGCGGCCTGCGGCCGTGCAGACAGAGGAGCAGTACAGGTTCCTGTACCACACGGTGG
NM_001142370.1      AGCAGCGGCCTGCGGCCGTGCAGACAGAGGAGCAGTACAGGTTCCTGTACCACACGGTGG
                    ***.                        * *  .* .*..* : *:* * *... * :*.

HsCD00383311        CTGGGCTTTTCTGCATCACTCTGATAAAGGAGAAGTGGCTGAATGAGGACATCATG----
NM_014369.3         CTCAGATGTTCTGCTCCACACTCCAGAATGCCAGCCCCCACTACCAGAACATCAAAGAGA
NM_001142370.1      CTCAGATGTTCTGCTCCACACTCCAGAATGCCAGCCCCCACTACCAGAACATCAAAGAGA
                    ** .*.* ******: ***:** .:.** *. *.    *: :*  **.******:.    

HsCD00383311        --------CTCAGGACCCTCAAGGTCACATTCCAGAAGGAGTCCCGTTCTGTGTACCAGC
NM_014369.3         ATTGTGCCCCACTCTACGACGATGCCCTCTTCCTCCGGACTCCCCAGGCACTTCTCGCCA
NM_001142370.1      ATTGTGCCCCACTCTACGACGATGCCCTCTTCCTCCGGACTCCCCAGGCACTTCTCGCCA
                            * ..  :.* :*.* * *. .****: ..*..  ***.  *: *  :* . .

HsCD00383311        TACAGTATATGTCCTGGCCAGACCGTGGGGTCCCCAGCAGTCCTGACCACATGCTCGCCA
NM_014369.3         TACCCCGCCCACCAGGAGGGGTCCTCAGGAGCATCTCTGTGCCCGGGTCCCCGGGCCACG
NM_001142370.1      TACCCCGCCCACCAGGAGGGGTCCTCAGGAGCATCTCTGTGCCCGGGTCCCCGGGCCACG
                    ***.  . . . *. *.  .*:**  .**. *. *:  .  ** *.  .*. *  * .*.

HsCD00383311        TGGTGGAGGAAGCCCGTCGCCTCCAGGGATCTGGCCCTGAACCCCTCTGTGTCCACTGCA
NM_014369.3         CCATGGCTGACACCTACGCGGTGGTGCAGAAGCGCGGGGCTCCAGCGGGCGCCGGGAGTG
NM_001142370.1      CCATGGCTGACACCTACGCGGTGGTGCAGAAGCGCGGGGCTCCAGCGGGCGCCGGGAGTG
                      .***. **..** .     *  :* ..:.  **   *.:**.    * * * . :* .

HsCD00383311        GTGCGGGTTGTGGGCGAACAGGCGTCCTGTGCACCGTGGATTATGTGAGGCAGCTGCTCC
NM_014369.3         GGACGCAGACGGGGACGGGGACGGGGACGGGGGCGCGCAGCGCGGAGGAGGCGCCGCTCT
NM_001142370.1      GGACGCAGACGGGGACGGGGACGGGGACGGGGGCGCGCAGCGCGGAGGAGGCGCCGCTCT
                    * .** . :  ***. .. ..  *  . * * .*    ..  . *:*..* .** **** 

HsCD00383311        TGACCCAGATGATCCCACCTGACTTCAGTCTCTTTGATGTGGTCCTTAAGATGAGGAAGC
NM_014369.3         ACAGCAAGGTGACGCCGCGCGCCCAGCGACCCGGGGCGCACGCGGAGGACGCGAGGGGGA
NM_001142370.1      ACAGCAAGGTGACGCCGCGCGCCCAGCGACCCGGGGCGCACGCGGAGGACGCGAGGGGGA
                    : * *.**.***  **.*  *.* : .*:* *   *.  : *   : .* . ****..*.

HsCD00383311        AGCGGCCTGCGGCCGTGCAG-------ACAGAGGAGCAGTACAGGTTCCTGTACCACACG
NM_014369.3         CGCTGCCTGGCCGCGTTCCTGCTGACCAAAGTCCTGCCGGATCTGGCGCCTACGAGGACG
NM_001142370.1      CGCTGCCTGGCCGCGTTCCTGCTGACCAAAGTCCTGCCGGATCTGGCGCCTACGAGGACG
                    .** *****    *** *.        *.**:  :**.* * . *   *  :. .. ***

HsCD00383311        GTGGCTCAGATGTTCTGCTCCACACTCCAGAATGCCAGCGAAGGGCATCATCACCATCAC
NM_014369.3         TGGCGGGTGGAGCTCAGACCGGTGGGCTAGGTTTCAACCTGCGCATTGGGAGGCCGAAGG
NM_001142370.1      TGGCGGGTGGAGCTCAGACCGGTGGGCTAGGTTTCAACCTGCGCATTGGGAGGCCGAAGG
                      *    :*.:* **:*. * . .  * **.:* *.* * ..* . :  .: .**.:.. 

HsCD00383311        CACTGATCCGAATTCGAGCTCCGTCGACA---------
NM_014369.3         GTCCCCGGGACCCGCCTGCTGAGTGGACCCGGGTGTAA
NM_001142370.1      GTCCCCGGGACCCGCCTGCTGAGTGGACCCGGGTGTAA
                     :*  .   ...  * :*** .** ***.

Pathways

WikiPathway :

B Cell Receptor Signaling Pathway

Protein

SMART domain :

PTPc_motif : Protein tyrosine phosphatase, catalytic domain motif

SMART domain :

PTPc : Protein tyrosine phosphatase, catalytic domain

Structural Annotation Wiki :

TOPSAN

UniProt :

Q99952

UniProt :

B4E1E6

HPRD :

05961

Clone

Cloning Information :

474882

HIP Master Clone ID :

396388

Original Clone ID :

8631a2BCt2p1

Experimental

TargetTrack - Experimental data :

NYSGXRC-8631a

Organism

Species Specific ID:

26469

Genome

Chromosome :

Map Location :

2q21.1

Ensembl :

ENSG00000072135

Reagents

Labome :

PTPN18-antibody

PTPN18 (H. sapiens )In pSGX3 (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

PTPN18 (H. sapiens )
In pSGX3 (His-tagged bacterial expression vector)