A0GQ56 (P. phytofirmans PsJN )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
11012b1BCt13p1
Keyword:
full-length cds
Species:
Paraburkholderia phytofirmans PsJN
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified. Actual 5' flanking sequence provided below.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
BpCD00383350
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1008nts
Open reading frame: 41 to 1048

TATTTTGTTAACTTTAGAAGGAGATATACATATGTCCCTTGCTTTTATGCAAGATGTGGC
CACGCTGGCTGGCGTCGCCCCGATGACGGTAAGCAATTGCTATCGTGTCCTGGACAAGGT
TCACCCGCAAACACTGGCCCGCGTCCTGGAGGCTGCAGCCAAGCTGGGTTACACCCGTAA
TCGCATTGCTGGGAATCTGGCGTCCGGCCGTAGTCAAGTAGTCGGCGTCCTGGTTCCGTC
ACTGGAGAACTCCAATTTTATCGGGATGATTCAAGGGCTGGAGGACCGCCTGCATCAGCT
GGACCATCAGCTGATGCTGGCAGTGACGCCGACGGCTGATCGTGAACTGGACTCAATCCG
CACCTTCATTGAACGTCGCATTGACGGCGTTGTTCTGACTGATGTTCAACACAGCCGTGA
CACGGTGGATCTGCTGCGCAATGCTCGTCTGCCGACTGTAGAGACTTGGTCGCTGAAAGG
CCCGTTTATCGATATGGGGGTAGGGTTTTCACTGTACGATGCAGCTTTTGATATGACAAA
CCTGATGATCGAAGCTGGTTACCGCCGTATTGGCTTCGCGGGCTTTACTCCGGCTTCAAC
CGAACGCTTCCTGGAGCGTCAGCGCGGTTTTGAAGATGCACTGCGTCGCGCGAATCTGCG
CCATGATGTACACGCTTACACGGAGCAATCCGAGGGCTTTGCTGGGGGCCGTATCACACT
GAATGCCCTGCTGCGCGAAGAGCCGGAACTGGACGGGGTGTTCTGCGGGACCGACATTCT
GGCTGCCGGTATGATTTTTGAATGTATGCGCCGCGGCTGGCGTGTGCCGGGTCGCATCGG
GATTGCTGGCTACGGTAACTACCAAATCGCATCGGAAATCACACCGAAACTGACCACAGT
TGCGACACCGGGGTATGAGATGGGGGTGGCTGCGGCAGAAATGCTGGTTGGCCGCCTGGT
GGGCGCAGCCCCGGCGCCGGTGATTCGTGACGTGGGTTATCGTCTGATCAGCGGGGCGTC
TGAAGGGCATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGG
CCGCACTCGAGCACCACCA

Protein sequence predicted based on nucleotide sequence

AFMQDVATLAGVAPMTVSNCYRVLDKVHPQTLARVLEAAAKLGYTRNRIAGNLASGRSQV
VGVLVPSLENSNFIGMIQGLEDRLHQLDHQLMLAVTPTADRELDSIRTFIERRIDGVVLT
DVQHSRDTVDLLRNARLPTVETWSLKGPFIDMGVGFSLYDAAFDMTNLMIEAGYRRIGFA
GFTPASTERFLERQRGFEDALRRANLRHDVHAYTEQSEGFAGGRITLNALLREEPELDGV
FCGTDILAAGMIFECMRRGWRVPGRIGIAGYGNYQIASEITPKLTTVATPGYEMGVAAAE
MLVGRLVGAAPAPVIRDVGYRLISGASEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g3t,A1A;a4t,c5t,T2F;
c71t,P24L;a834g,S278S
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-11012b
Recommended expression in:
Not Applicable