Gene:
Gene Symbol:
Mfla_2672
Gene Name:
replication restart DNA helicase PriA
Original Clone ID:
10454w2BCt6p1
Keyword:
full-length cds
Species:
Methylobacillus flagellatus KT
Type:
cDNA
Vector Name:
pSGX3
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified. Actual 5' flanking sequence provided below.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
MfCD00383511
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1647nts
Open reading frame: 51 to 1697
TTCCTCTGAATATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTGCTTCCAGCG
TCCAGCCAGAGTTGAATGAAGAGCAAGTCCTGGCCGTACAGAGCATCCTGCAGTATCGGG
ATACCTTCAGGCCGTGGCTGCTGCACGGCATCACCGGCAGCGGCAAGACCGAGGTATACA
TCCGCCTGCTACAGGCAGTGCTGGAGGGTAATCAGGACCAGGCGTTGGTGCTGGTGCCGG
AAATCAACCTTACCCCACAGCTGGAAGCACGCTTCCGTACGCGGTTGCCGCAATTCCCGC
TGGTTACCCTCCACAGCAATCTCAGCGAGAGTGAGCGCTTGCAGAACTGGCGCCTGGCGC
AGACTGGTGCGGCACGCATTGTCATCGGCACGCGCCTGTCGGTGTTCACCCCGCTGCCCG
GGCTCAAATTCATGATCGTGGACGAGGAGCACGACACTTCCTACAAGCAGCAGGACGGCA
TGCGCTACCATGCCCGAGATGTGGCCCTGGTGCGCGCGCAACGCCGCAACATTCCCATCG
TGCTGGGCTCGGCCACCCCCGCACTGGAAACCTGGCATAACGCCCGCAATGGGCGCTATG
GCCTGCTCACCTTGCAGCAGCGGGCGGTGAGCCAATCCCAGCTGCCGCAAGTCAATTGCG
TGGACGTTTCCAGGGTGCAGATGCAGGAAGGCTTGTCGCCGCAGCTGCTCAAGGCACTGA
AAGTTCGGCTGGAGCGTGGTGAGCAGAGCCTGCTCTTCATCAATCGCCGCGGCTATGCGC
CGGTGCTGCTGTGCAATGCCTGTCACTGGATTGCACCCTGCATGCGTTGCAGCAGTCGGC
TGGTGGTCCACCTGCGCCAGGGATTCCTGCGCTGCCATCATTGTGGACATGAGCAGAACA
TCGTGCGGCAATGTCCGAGTTGCGGCAATGCCGACCTTCACCCCACGGGGAATGGCACGC
AGCGCCTGGAGGAAACCCTGATCAGGCTGCTGCCTGAAGCCCGTGTATTGCGGGTTGACC
GTGACAGTACGCGCCGCAAGCACGCATTGAGCGAGATGTTGTCTGCGGTCCATGTAGGCG
AGGTGGATATCCTGGTGGGGACGCAAATGCTGGCGAAAGGCCACGATTTCCCCAACCTGA
CGCTGGTGGGCGTGCTCGATACCGACAGTGCCCTGTTCAGTCCTGATTTCCGTGCTGCCG
AGCGCCTGTTTGCCCAGCTCATGCAGGTGGCGGGGCGGGCTGGCCGGGCGGATAAGCCAG
GCGAGGTGCTGATCCAGACAAGTTTTCCCGAGCACCAGCTGTTCAATGCCCTGCGCGTCC
AGGATTTTGCAGCCTACGCCGCCAGCCTGCTGCAGGAGCGGGAAGTTGCCCAGTTCCCTC
CCTGCGAATTCCTGGCCTTGATCAAGGCCGAGGCCAATGAATATGGTCCCGTGCAGCGCT
TCCTGCAGTTTGCTGCCGATAGCGCGCGAGCGCTAGGGACCGACGCATTGGTCTATGACC
CGGTCCGACCACAAATGGAGCGCCTCAAAGGCATGGAACGCGGGCAGTTGCTGTTCCAGG
CCGCGACCAGGCAAACCCTGCAGATAGTCCTCAGGCAATTGACGCCGCAGCTGCGTGCCC
ATCCACTCAGCAACAAGATACGCTGGGCGGTGGACGTGGATCCGCAGGAGGAAGGGCATC
ATCACCATCACCACTGATCCGAATTCGAGCTCCGTC
Protein sequence predicted based on nucleotide sequence
ASSVQPELNEEQVLAVQSILQYRDTFRPWLLHGITGSGKTEVYIRLLQAVLEGNQDQALV LVPEINLTPQLEARFRTRLPQFPLVTLHSNLSESERLQNWRLAQTGAARIVIGTRLSVFT PLPGLKFMIVDEEHDTSYKQQDGMRYHARDVALVRAQRRNIPIVLGSATPALETWHNARN GRYGLLTLQQRAVSQSQLPQVNCVDVSRVQMQEGLSPQLLKALKVRLERGEQSLLFINRR GYAPVLLCNACHWIAPCMRCSSRLVVHLRQGFLRCHHCGHEQNIVRQCPSCGNADLHPTG NGTQRLEETLIRLLPEARVLRVDRDSTRRKHALSEMLSAVHVGEVDILVGTQMLAKGHDF PNLTLVGVLDTDSALFSPDFRAAERLFAQLMQVAGRAGRADKPGEVLIQTSFPEHQLFNA LRVQDFAAYAASLLQEREVAQFPPCEFLALIKAEANEYGPVQRFLQFAADSARALGTDAL VYDPVRPQMERLKGMERGQLLFQAATRQTLQIVLRQLTPQLRAHPLSNKIRWAVDVDPQE EGHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c863t,T288I
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-10454w
Recommended expression in:
Not Applicable
Vector Name:
pSGX3
Synonyms:
pSGX-3, pSGX3_BC
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen
Size (bp):
5339
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation start (Met, Ser, Leu)
5072
5079
TOPO recognition site
TOPO
TOPO cloning site
5076
5085
bacterial origin
ori
pBR322-type bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4983
5003
repressor protein gene
LacI
LacI CDS
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5086
5103
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5231
5307
