Rmet_4950 (C. metallidurans CH34 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Rmet_4950
Gene Name:  LacI family transcriptional regulator
Original Clone ID:
11025j1BCt4p1
Keyword:
full-length cds
Species:
Cupriavidus metallidurans CH34
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified. Actual 5' flanking sequence provided below.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
RmCD00383529
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1038nts
Open reading frame: 38 to 1075

TTGTTTACTTTAAGAAGGAGATATACATATGTCCCTTAAACAGATTAGCATCCGTGACGT
GGCGGCCCTGGCAGGCGTTTCTGTCGGTAGTGCTTCACGCGTAATTAACGGGGCGGCCAA
CGTGACACCGGACGTTCGTGAACGCGTCGATCGTGCAATCGCTGCGCTGCGCTATCGTCC
GAATCACGCCGCACAATCGCTGCGTTCACGTACGAGCAAGACTATTGGCTGTATGTTTTC
TGACGTAGCTAACCCGCTGTACGCGCGCGCCTTCCGTGCACTGGAGGAACACTTTCGCCG
TGAAGGTTACATGCTGCTGCTGGCTAATGGGCTGAATGATGCGGAACGCGAAATCGACAT
TATCCAGACATTCCAACTGCGTGGCATGGATGGTGTGATTCTGGCCCCGGGCAATGAGCG
CCATCCGGGTGTTCTGGACGCAGTTCGTCGCCTGAGTATGCCGGCGGTGATTTACGATCG
CGATCTGGCTGTGGATGCAGACGCTGTACTGTTTGACCACGTGGCGGGGGTCAAAGCTGC
CATTCACCACCTGGTTGAACTGGGCCACACGCGTATCGCACTGGCGCTGTGGCAAGTAAA
ATCTCGCCCGGTCCGCCGTCGCATCGAGGGCTATAAAGCTGCCTTCAAAGCAGCGGGCCT
GCCGGTCCCGGACCTGATCCTGCAGGAACCGACCCCGACTTCCTCGGCCTTTGATGACGT
TACACGTCTGCTGCAACTGCCGGACCCGCCGACCGCATTCCTGGCACAAGGCACGCACAC
CCTGACTAGCACACTGCGCGCTATTGCCAAACAAGGGCTGCGTGTGCCGGAGGACGTATC
TGTAATCGGGATTGGTGATACGGATTTCGCTCAAACATATGACCCGCCGCTGACTGTACT
GCGCACTCCGACCGATGAGGTGGCAGCGCACGCCGTAGAAATGCTGCTGTCGCGTCTGGC
GGCTAAGGGGGACGCATCACTGTCCCCGCGCAAAGCCCTGATTCAGTTCGATCTGATTCT
GCGTGAATCTTGTGCACCGGTTGCTCGCGAAGGGCATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

KQISIRDVAALAGVSVGSASRVINGAANVTPDVRERVDRAIAALRYRPNHAAQSLRSRTS
KTIGCMFSDVANPLYARAFRALEEHFRREGYMLLLANGLNDAEREIDIIQTFQLRGMDGV
ILAPGNERHPGVLDAVRRLSMPAVIYDRDLAVDADAVLFDHVAGVKAAIHHLVELGHTRI
ALALWQVKSRPVRRRIEGYKAAFKAAGLPVPDLILQEPTPTSSAFDDVTRLLQLPDPPTA
FLAQGTHTLTSTLRAIAKQGLRVPEDVSVIGIGDTDFAQTYDPPLTVLRTPTDEVAAHAV
EMLLSRLAAKGDASLSPRKALIQFDLILRESCAPVAREGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a1025t,c1026t,H342L;c1027t,H343Y;
c1030a,H344N;c1033g,H345D
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-11025j
Recommended expression in:
Not Applicable