XP_308643 (A. gambiae )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
8996a1BCt4p1
Keyword:
full-length cds
Species:
Anopheles gambiae
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified. Actual 5' flanking sequence provided below.
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
AgCD00383537
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1434nts
Open reading frame: 38 to 1471

TTTGTTAACTTTAGAAGGAGATATACATATGTCCCTTGACGACGAGAGCTATGAGGAAGA
GCATAAACGTATGGTGCTGAAAATTCACCAGGAATTTTGGGACCTGCCGACCAATTACCA
AGAAAAACCACTGGTTTTCGGATCTCAGGCGAAAAACCGCTATAAAACGATCCTGCCTAA
TGAGCATAGTCGTGTCATTCTGGAACCGGAGCGCTCCCCAGCCGCAGGTGGGGAACCTTA
CATCAACGCTAATTATATTAAAGGACCGGATTACGCGAACAATTCCTATATCGCCACTCA
AGGCCCGCTGCCAAACACAATTTACGAATTTTGGCTGATGGTATATCAGAATGTGGTTCG
TACCGCAACGGTCGCTGGTCGCTCGTGTGCGTCTGCCGGACTGGAGCAGAAAATCGTAAT
GCTGACTAACTTCGTGGAAAATGGACGTCAAAAATGTGCAATTTATTTTCCTCAGACAGC
TGATGAGTGGGTTGCGTTTGGTAGTGGTGCCGACATCGATGTCGGCGAAATGCTGCGCGA
AAAAGATACCATTACGGCAAGTATCGCTGCGGCCGAGCAACAGCCGGAAGGTCAAGAGGC
AGTGGGGGGAGTATGCCAACCGGTTACCGTGCGTGTCCCATGCGCCTCGTGCTTTTTCCT
GGTTCACAACGTAGCTGTGGAACAGCGCAATGGCTACACTGTCCGTACACTGAACGTTAT
TTATGGTGCCAAAGTGTCCGACGCAGATGCGCCTGACGGCGATGGTTCGCGCGCTCTGTT
TGAACTGACGCAATTCCGTGCCCATCACTACTGGTTTCCGGACTGGCCAGATCATCGTAG
TCCGAAAGATATCAATGTACTGCTGGACCTGTCTCTGGATGTCCTGGGGAGTAACGTTGT
GGACACCCCTCGTCCGGAGTCATCCAATTCGCTGAAAGGAAACCAGCCAGGCACTCCGGG
TCTGGAAGAAGTAATGGGGGCAGCGCAGCTGGCTGCCCTGAGCTCTAGTTGTACACCTAC
CGTTCTGCCGATTGTGCACTCAAGCGCTGGCATCGGTCGCACGGGATGCCTGATTGCGAT
TCTGAATGGCCTGCGTCAACTGCGCCTGAGCGTCATGGCCCATCAGCGTAAACAACAGCA
GCTGAAACAAAGCCAAGCCCAAACCGGTGAGGCGGGGCAGGGCGAACTGCACGGTTCTGA
GGGACGCCGTAAAAGTACTGTTCAGATGGATGGCGCTACACTGCTGGCCCCAAACGCGGC
TGTGGGTCGCGGGGGACAACGTGAAGGCTCAGCAGTAGTCCGCGAAAGCGGTCAGGTTGA
TATCCTGGGGATTGTGTGTAATCTGCGTCTGCAACGCGGCGGTATGGTACAGAACTCCGA
GCAGTATGAACTGATCCATCGTGCCCTGTGCCTGTACCTGGAGAAACTGACGCAAGACGG
ACAGGAAGGGCATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

DDESYEEEHKRMVLKIHQEFWDLPTNYQEKPLVFGSQAKNRYKTILPNEHSRVILEPERS
PAAGGEPYINANYIKGPDYANNSYIATQGPLPNTIYEFWLMVYQNVVRTATVAGRSCASA
GLEQKIVMLTNFVENGRQKCAIYFPQTADEWVAFGSGADIDVGEMLREKDTITASIAAAE
QQPEGQEAVGGVCQPVTVRVPCASCFFLVHNVAVEQRNGYTVRTLNVIYGAKVSDADAPD
GDGSRALFELTQFRAHHYWFPDWPDHRSPKDINVLLDLSLDVLGSNVVDTPRPESSNSLK
GNQPGTPGLEEVMGAAQLAALSSSCTPTVLPIVHSSAGIGRTGCLIAILNGLRQLRLSVM
AHQRKQQQLKQSQAQTGEAGQGELHGSEGRRKSTVQMDGATLLAPNAAVGRGGQREGSAV
VRESGQVDILGIVCNLRLQRGGMVQNSEQYELIHRALCLYLEKLTQDGQEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
C448S

Discrepancy:
a1421t,c1422t,H474L;c1423t,H475Y;
c1428g,H476Q;a1430g,H477R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in NYSGXRC-8996a
Recommended expression in:
Not Applicable