BF2339 (B. fragilis NCTC 9343 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BF2339
Gene Name:  amylase
Original Clone ID:
11099a1BCt4p1
Keyword:
None
Species:
Bacteroides fragilis NCTC 9343
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified. Actual 5' flanking sequence provided below.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
BfCD00383631
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1716nts
Open reading frame: 51 to 1766

TCCCTCAGAAAATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTAAGAACGAAA
ACAAAATGATTATTTATCAAGTGTTTACACGCTTGTTTGGTAATAATAATAATCACTGTA
TCTACAATGGAGATATTTCACAAAATGGTTGTGGAAAAATGGCTGATTTTACTGCCAAAG
CTTTGGGAGAAATAAAAAAACTGGGAGCAACACATATTTGGTACACGGGTATTATCGAAC
ATGCCAGCCAAACTGATTATAGAAGATACAACATCCGTCCGGATCATCCGGCTATTGTAA
AAGGTAAAGCGGGATCTCCATACGCCATTAAAGATTATTATGATGTAGATCCGGATCTGG
CTACTGATGTCCCTGGAAGAATGAAAGAATTCGAAAATCTAGTAAGTCGTACACACAGAG
CAGGATTAAAAGTAATAATTGATTTTGTTCCGAACCATGTAGCGCGTCAATACCATTCGG
ATGCACAACCTGACGGCACCACTCAGCTGGGAGCCAATGATGATCCTAACTACTCTTTTA
GTCCGTACAATAATTTCTACTATATTCCGCAATCGGAATTGCATGGACAGTTTGATATGA
CGGGAAATGCCTTGGAACCCTATCATGAATTTCCTGCCAAAGCAACCGGAAATAACCGTT
TTGATGCTTACCCTAATATTAATGACTGGTATGAAACCGTAAAGTTAAATTATGGTGTGG
ATTATCAGAATGGTGGAACTTGCCATTTCTCCCCTACTCCGGATACTTGGACTAAAATGT
TGGATATCCTCCTTTTTTGGTCCTCTAAAAATATTGATGGTTTCCGTTGTGACATGGCCG
AAATGGTTCCGGTAGAATTTTGGGAATGGGCTATCCCTCAAGTGAAACAGGAGTATCCGA
ATATTATATTTATTGCTGAAGTATACAATCCGCACGAATATAAGAATTATTTATTTCGTG
GTAAATTTGATTTTCTCTACGATAAAGTAGGACTGTATGATACATTGCGCAATGTAGCTT
GTGGTTATGACTCTGCAACTGCTATTACTCGTAGTTGGCAGTCTTTAGGGGGGATTGAAA
AGCGGATGCTTAACTTCCTTGAAAACCATGACGAACAACGTATTGCATCTGATTTTTTTG
CCGGAGATCCACGCAAAGGTGTTCCTGCCTTAATTGTATCTGCTTGCATGAATACTAACC
CCATGATGATCTACTTTGGTCAGGAATTCGGAGAAATGGGAATGGATAGTGAAGGTTTCA
GTGGACGTGATGGACGTACTACTATTTTCGATTATTGGAGTGTAGATACAATTCGGCGCT
GGCGAAATGAAGGAAAGTTTGACGGGAAGATGCTAACTGAAGAGCAAAAACATTTATATG
CAATTTATCAGAGAGTTTTGACGTTGTGCAATGAAGAACAGGCAATATCAAACGGCGTAT
TCTTTGATTTGATGTATGCTAATGAAAATGGATGGAGATTTAATGAGCACAAGCAATATA
CATTTATGCGTAAATACAAGAATGAATTGCTATTTATTGTCGTAAACTTTGATAATCAGC
CAGTAAATGTTGCGATTAATGTGCCTTCTCATGCCTTTGACTTTTTACAAATTCCTCAAT
TTGATTCTTATAAAGCGGTTGATTTACTAACAGATAAAGTAGAAGAAATCAGTTTACTGC
CATATAAGGCAACAGAAATCGCTTTAGGAGCTTATACGGGTAAAATATTGAAGATTAAAG
AAGGGCATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTCGACA

Protein sequence predicted based on nucleotide sequence

KNENKMIIYQVFTRLFGNNNNHCIYNGDISQNGCGKMADFTAKALGEIKKLGATHIWYTG
IIEHASQTDYRRYNIRPDHPAIVKGKAGSPYAIKDYYDVDPDLATDVPGRMKEFENLVSR
THRAGLKVIIDFVPNHVARQYHSDAQPDGTTQLGANDDPNYSFSPYNNFYYIPQSELHGQ
FDMTGNALEPYHEFPAKATGNNRFDAYPNINDWYETVKLNYGVDYQNGGTCHFSPTPDTW
TKMLDILLFWSSKNIDGFRCDMAEMVPVEFWEWAIPQVKQEYPNIIFIAEVYNPHEYKNY
LFRGKFDFLYDKVGLYDTLRNVACGYDSATAITRSWQSLGGIEKRMLNFLENHDEQRIAS
DFFAGDPRKGVPALIVSACMNTNPMMIYFGQEFGEMGMDSEGFSGRDGRTTIFDYWSVDT
IRRWRNEGKFDGKMLTEEQKHLYAIYQRVLTLCNEEQAISNGVFFDLMYANENGWRFNEH
KQYTFMRKYKNELLFIVVNFDNQPVNVAINVPSHAFDFLQIPQFDSYKAVDLLTDKVEEI
SLLPYKATEIALGAYTGKILKIKEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11099a
Recommended expression in:
Not Applicable