pulA (B. adolescentis ATCC 15703 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  pulA
Gene Name:  pullulanase
Original Clone ID:
11097m1BCt9p1
Keyword:
None
Species:
Bifidobacterium adolescentis ATCC 15703
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified. Actual 5' flanking sequence provided below.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
BbCD00383632
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1410nts
Open reading frame: 51 to 1460

TTCCCCTGAATATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTGACCTGCCGC
TTGCGCAGACGGTGGTCTGGGAAACCCATGTGGGCGATTTCAGCAATGATCCCGCCGGCG
GTTTCCCCGAATCGCACCGCGGCAAGTATCTTGCGTTCACCGATGACGGTACGTCGTTGG
AAGGCCATCCCGACTTTCCGACCGGCCTTGCGTACCTGAAGAAGCTTGGTGTCACTGCAG
TGCAGCTGATGCCGATTTACGACTTCGGTTCAGTGGACGAAACGACCTGCGACCGCTATA
ACTGGGGCTACGATCCGATCAATTACAATGTGCCGGAAGGCTCGTACTCCACCAATCCGT
TCGACGGCACCACACGAATCCGCGAAGTCAAGCAGATGGTGCAGGCGCTGCACAGTGCGG
GCATCAAGGTGATCATGGATGTGGTTTACAACCACATGTTCTCCACCGACAATTGGTTCG
AACGTACGGTGCCGAAATACTTCCTGCGCCGCAAGCCCAACGGCGCGCTGGCCAACGGAT
CCGGCTGCGGCTGCGATATGGCCACCGAACGCAAGATGTTCCGCCGGTTCATCGTGGAAT
CCGTGACGTTCTGGGCGCGCGAATACCACATGGACGGCTTCCGCTTCGATCTGATGGGCC
TGATCGACGTGGACACCATGAACGCCGTGCGCGCCGCGTTGAACGAGATTCCCGGCCGCG
GCCGTGACATCCTCATGTATGGCGAACCGTGGTCCGCAGGCGCGTCGGCGCCACTTCCCG
CGGACACGCTGCTCGCCGGCAAGCAGGGGCTTGCGCAGTTGGACGATCGCATCGGCCACT
TCTGCGACACCACGCGCGACACCATCAAAGGCCACGTGTTCTATTCGGATCGCCCCGGCT
ACGTGAACGGCGGCATGCACGCCAACGCCGTGGCCGTGCGCGAGGCGGTGGACGCCTGGC
GCCATAGCGACCGGCCGGAAGGCGTTGCTGGGCAGGTGATCCAATACGTTTCCGCGCATG
ATGACCTGACCTTGTGGGACAAGCTATGCGCCAGTCTTGCGGCGAGCTCGCTGGGCAGCG
CCGTGGCCGAAGGCGACGAGGAAAACACGGTGGATGTGCCGAAGACGCTGCATGACGCCG
ATTTCTCCGAACAGGGACTGGACGCCGTCGGACCGGAGATGGCACAGGCGTTGCACGATG
TGCTGGGGGCCAATCGTCTGTCCGCCGGCATCGTGCTCACATCGGCAGGCATTCCGTTCA
TGCTGTCCGGCGAGGAGTTCGCGCGCACCAAATACGGCAATTCCGATTCGTACGATTCGA
CCAAGGAGCTCAACTGGCTGGATTGGAACCGCGCTTGGCATATGCGCGATCTGATCGGTT
TCTATGCGAAGCTGATCGCCCTGCGCAAAAGCGATGCGCGATGGTTCGACGGCGAAGGGC
ATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTCGACAA

Protein sequence predicted based on nucleotide sequence

DLPLAQTVVWETHVGDFSNDPAGGFPESHRGKYLAFTDDGTSLEGHPDFPTGLAYLKKLG
VTAVQLMPIYDFGSVDETTCDRYNWGYDPINYNVPEGSYSTNPFDGTTRIREVKQMVQAL
HSAGIKVIMDVVYNHMFSTDNWFERTVPKYFLRRKPNGALANGSGCGCDMATERKMFRRF
IVESVTFWAREYHMDGFRFDLMGLIDVDTMNAVRAALNEIPGRGRDILMYGEPWSAGASA
PLPADTLLAGKQGLAQLDDRIGHFCDTTRDTIKGHVFYSDRPGYVNGGMHANAVAVREAV
DAWRHSDRPEGVAGQVIQYVSAHDDLTLWDKLCASLAASSLGSAVAEGDEENTVDVPKTL
HDADFSEQGLDAVGPEMAQALHDVLGANRLSAGIVLTSAGIPFMLSGEEFARTKYGNSDS
YDSTKELNWLDWNRAWHMRDLIGFYAKLIALRKSDARWFDGEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g4c,V2L
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-11097m
Recommended expression in:
Not Applicable