catB (P. fluorescens )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  catB
Gene Name:  muconate cycloisomerase
Original Clone ID:
9450f2BCt5p1
Keyword:
None
Species:
Pseudomonas fluorescens
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified. Actual 5' flanking sequence provided below.
Mutation / Discrepancy:
No / No
Publications:
PMID: 19220063
Title: Evolution of enzymatic activities in the enolase superfamily: stereochemically distinct mechanisms in two families of cis,cis-muconate lactonizing enzymes
Authors:
PSI
New York SGX Research Center for Structural Genomics
PfCD00383653
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1140nts
Open reading frame: 51 to 1190

TTCCTCTAAATATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTCACGCCTCTG
ACATTGAATCGATCGAGACGATCATCGTCGATCTGCCGACCATCCGCCCCCACAAGCTGG
CCATGCACACCATGCAGAACCAGACCCTGGTGCTGATCCGCCTGCGCTGCGCCGACGGCA
TCGAAGGCCTGGGGGAATCCACCACCATCGGTGGCCTGGCCTATGGCAATGAAAGCCCGG
ACAGCATCAAGACCAATATCGACCGTTTCGTCGCGCCGCTGCTGATCGGCCAGGACGCCA
GCAACATCAACGCCGCCATGCTGCGCCTGGAGCAGAGCATCCGCGGCAACACCTTCGCCA
AATCCGGTATCGAAAGCGCCTTGCTCGACGCCCAGGGCAAGCGCCTCGGGCTGCCGGTCA
GCGAACTGCTGGGCGGCCGGGTGCGCGATGCGCTGCCGGTGGCCTGGACCCTGGCCAGCG
GCGACACCGCCAAGGACATCGCCGAAGCGCAGAAGATGCTCGACCTGCGCCGCCACCGCA
TCTTCAAGCTGAAGATCGGCGCCGGTGAAGTGGACCGCGACCTGGCCCACGTGATCGCCA
TCAAGAAGGCCCTGGGCGACAGCGCCAGCGTGCGGGTCGACGTCAACCAGGCCTGGGACG
AAGCCGTGGCCCTGCGCGCCTGCCGCATCCTCGGCGGCAATGGCATCGACCTGATCGAAC
AACCGATTTCGCGCAACAACCGCGCCGGCATGGTGCGCCTGAATGCCAGCAGCCCGGCGC
CTATCATGGCCGACGAATCCATCGAGTGCGTGGAAGACGCCTTCAACCTGGCCCGGGAAG
GGGCGGCTTCGGTGTTCGCCCTGAAGATCGCCAAGAACGGCGGTCCTCGCGCCACCCTGC
GCACCGCGGCGATTGCCGAGGCGGCGGGCATCGGCCTGTACGGCGGCACCATGCTCGAGG
GCGGCATCGGCACCCTGGCCTCGGCCCACGCCTTCCTGACCCTGAACAAACTGAGCTGGG
ACACCGAACTGTTCGGCCCGCTGCTGCTGACCGAGGACATCCTCGCCGAGCCGCCGGTGT
ACCGGGATTTCCACCTGCATGTCTCAAAGGCACCAGGCCTTGGCCTGAGCCTGGACGAAG
AGCGCCTGGCGTTCTTCCGTCGCGAAGGGCATCATCACCATCACCACTGATCCGAATTCG
AGCTCC

Protein sequence predicted based on nucleotide sequence

HASDIESIETIIVDLPTIRPHKLAMHTMQNQTLVLIRLRCADGIEGLGESTTIGGLAYGN
ESPDSIKTNIDRFVAPLLIGQDASNINAAMLRLEQSIRGNTFAKSGIESALLDAQGKRLG
LPVSELLGGRVRDALPVAWTLASGDTAKDIAEAQKMLDLRRHRIFKLKIGAGEVDRDLAH
VIAIKKALGDSASVRVDVNQAWDEAVALRACRILGGNGIDLIEQPISRNNRAGMVRLNAS
SPAPIMADESIECVEDAFNLAREGAASVFALKIAKNGGPRATLRTAAIAEAAGIGLYGGT
MLEGGIGTLASAHAFLTLNKLSWDTELFGPLLLTEDILAEPPVYRDFHLHVSKAPGLGLS
LDEERLAFFRREGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t6c,A2A;c11a,A4D
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-9450f
Recommended expression in:
Not Applicable