pde191 (A. thaliana Columbia )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  pde191
Gene Name:  protein PIGMENT DEFECTIVE 191
Original Clone ID:
6001.1.22_GO.19158
Keyword:
full-length cds
Species:
Arabidopsis thaliana Columbia
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Center for Eukaryotic Structural Genomics
AtCD00384071
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1020nts
Open reading frame: 51 to 1070

ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCCGT
ACTTCCAGTCCGAGGTGACAAATACGAGCAGCATCATGTGGTTCTTCAGGGACAAAGGTT
TCGATGATCCGAGTATCGACAAGATGTTGAGAAAATGCAAACAGTTGGAAAAGGCTCAAA
GCGATGTGGCATCAGAAAACTGGGACTACCTGAGTAACATTGTTGGTATCCAAGAGAGAA
AACTCCCTTACATCGTCTCTCGATGCCCCAAAATCCTGACTTTACGCCTCGATGAGAGAC
TCATCCCGATGGTCGAGTGCCTCTCCAGTCTTGGAAGGAATCCTCGGGTAGTTGCTTCCG
CCATTACCAAATTTCCTCCAATACTCTCTCATAGCGTGGAGGAGAAACTCTGTCCCCTTC
TTGCTTTCTTTCAAGCGTTAGGTGTGCCTGAGACTCAACTTGGCAAAATGATACTTTTTA
ACCCAAGGCTTATCAGCTACAGCATCGACACCAAGCTGACAGTGATCGTCAGCTTTCTTG
CTAGCCTTGGCCTTGATCAAGATGGGATGATTGGCAAAGTTCTGGTGAAGAACCCATTTC
TTATGGGGTACAGTGTTGATAAAAGGTTACGGCCTACCACTGAATTCTTGAAATCATCCG
TTGGCCTGAGTGAGGATGGCATTAAGTCGGTGGTCATGAATTTCCCACAACTTTTGTGCA
GAGACGTTAACAAGATTCTCAAACCAAATTATGATTATTTGAAGGAGTGTGGGTTTGGAG
ATTCCCAGATAGCAACCATGGTCACTGGTTATCCCCAAATCTTGATTAAGAGTGTTAAGA
ATTCACTACAGCCTAGGATCAGATTCCTCGTCCAGGTGATGGGAAGAGGCATGGATGAAG
TGGCTTCTTATCCTGAATTCTTTCACCATGGATTGAAGAAGAAGGTCGAATCGAGGTTTA
AACTTGTCAAAAAGAACAACATTGACTGCAGCCTTAGAGAAATGCTGGACTGTAACACAA
AGAAATTCCATGAGAAGTTTGGCTTTTCGGAGGTAACTGCAAGCTTCTAGGACCCAGCTT
TCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCA

Protein sequence predicted based on nucleotide sequence

ENPYFQSEVTNTSSIMWFFRDKGFDDPSIDKMLRKCKQLEKAQSDVASENWDYLSNIVGI
QERKLPYIVSRCPKILTLRLDERLIPMVECLSSLGRNPRVVASAITKFPPILSHSVEEKL
CPLLAFFQALGVPETQLGKMILFNPRLISYSIDTKLTVIVSFLASLGLDQDGMIGKVLVK
NPFLMGYSVDKRLRPTTEFLKSSVGLSEDGIKSVVMNFPQLLCRDVNKILKPNYDYLKEC
GFGDSQIATMVTGYPQILIKSVKNSLQPRIRFLVQVMGRGMDEVASYPEFFHHGLKKKVE
SRFKLVKKNNIDCSLREMLDCNTKKFHEKFGFSEVTASF*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t8c,L3P;del@1018,3
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.19158
Recommended expression in:
Not Applicable