BALH_0898 (B. thuringiensis str. Al Hakam )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BALH_0898
Gene Name:  NADPH:quinone reductase (quinone oxidoreductase)
Original Clone ID:
11165b1BCt8p1
Keyword:
full-length cds 2-330 of 330 aa
Species:
Bacillus thuringiensis str. Al Hakam
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified. Actual 5' flanking sequence provided below.
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
BtCD00384151
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1014nts
Open reading frame: 28 to 1041

TAAGAAGGAGATATACATATGTCCCTTCACGGAAAACTCATTCTATTTCATAAGTTTGGC
AATCCAAAAGACGTATTACAAGTTGAATATAAAAATATAGAACCACTGAAAGATAATGAA
GTTTTCGTCCGTATGCTAGTTAGACCGATTAATCCATCTGACTTAATTCCCATCACAGGA
GCGTACGCTCATAGAATTCCTTTACCTAACATACCTGGCTATGAAGGTGTTGGTATTGTA
GAAAATGTTGGCGCTTTTGTTTCTCGAGAGCTTATCGGTAAACGAGTGTTACCTTTACGC
GGGGAAGGTACTTGGCAAGAATATGTGAAGACATCAGCTGATTTTGTAGTCCCTATTCCT
GATTCCATTGATGATTTCACAGCGGCACAAATGTATATTAATCCCCTTACGGCGTGGGTT
ACTTGTACAGAAACGTTAAACTTACAGCGTAATGACGTTTTATTAGTGAATGCTTGTGGG
TCCGCTATTGGACATCTATTTGCGCAGTTATCCCAAATTTTAAATTTCCGATTAATTGCC
GTTACAAGAAATAATAAGCATACAGAAGAATTACTGCGCCTTGGTGCTGCATATGTAATT
GATACTTCCACTGCCCCGCTTTATGAAACTGTTATGGAATTAACAAACGGGATTGGTGCG
GATGCTGCGATTGATTCTATCGGAGGACCGGATGGAAATGAATTAGCTTTTTCCTTACGT
CCAAACGGGCATTTTTTAACGATCGGTCTTCTATCAGGAATACAAGTGAACTGGGCCGAG
ATTGTCACAAAAGCAAAAGTGCACGCAAACATATTTCATTTACGACATTGGAATGATGAA
GTGTCACCATATAAATGGCAAGAAACGTTTCGTCACTTAATTCGTTTAGTAGAAAATGAG
CAATTACGTTTTATGAAAGTCCATTCTACGTATGAATTAGCGGATGTGAAAGCGGCAGTT
GATGTTGTACAGTCTGCTGAGAAAACGAAAGGTAAAGTGTTTTTGACGAGTTATGAAGGG
CATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTCG

Protein sequence predicted based on nucleotide sequence

HGKLILFHKFGNPKDVLQVEYKNIEPLKDNEVFVRMLVRPINPSDLIPITGAYAHRIPLP
NIPGYEGVGIVENVGAFVSRELIGKRVLPLRGEGTWQEYVKTSADFVVPIPDSIDDFTAA
QMYINPLTAWVTCTETLNLQRNDVLLVNACGSAIGHLFAQLSQILNFRLIAVTRNNKHTE
ELLRLGAAYVIDTSTAPLYETVMELTNGIGADAAIDSIGGPDGNELAFSLRPNGHFLTIG
LLSGIQVNWAEIVTKAKVHANIFHLRHWNDEVSPYKWQETFRHLIRLVENEQLRFMKVHS
TYELADVKAAVDVVQSAEKTKGKVFLTSYEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
Q9H; L34F; D74N; G78F; T80S; S81R; D82E; T205E; L210I; A226E; V246I; K271D; D272E; P274S; K292E; D304E

Discrepancy:
a17t,Q6L
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11165b
Recommended expression in:
Not Applicable