Sputcn32_3003 (S. putrefaciens CN-32 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Sputcn32_3003
Gene Name:  GntR family transcriptional regulator
Original Clone ID:
44599
Keyword:
full-length cds
Species:
Shewanella putrefaciens CN-32
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
SpCD00384321
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1464nts
Open reading frame: 51 to 1514

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGAAAAAAC
CTCAATCCAATTTCCCTCACTTGCAACTCAAAGATGGCATCCTCAAAGATCAGCTTTATC
ATGCGTTTAGAGAGGCCATTCTTGATGGACGTTTGCACCCGCGAATGAAGCTCCCATCCA
GCAGAGCCTTGTCGGAAACCATGCAGATTTCACGTAACTCAGTACTGCAAGGGTTTGAGC
GCCTGATTGATGAAGGCTATCTGCTGACTAAACCGGGATCGGGCACTTATGTGTCAGATA
CCATCCCCGATGAACTGATCGATATTCCCAACAGCTCCCGAGAAAGTGCTCAAGCCTTTG
ATTCCAATGTAGACATTAATCCACAGTTAATAGAAATGGCGCGACTGAAAGCGAAGGGAA
ATACGGACCACAGCCATAGCATGCTTAATATTGGCGTCGGATGTGTCGATCTTTTCCCAC
ACCAACTTTGGGGACGGCTGTTAGGCCGAGTTTGGCGGCAATCGCGTAAAACCTTAGGTA
AATCAAACCATAGCGCGGGGTATTTGCCGTTGCGTGAGGCGATAAGTGACTATGCGCGTT
CGACCCGCGGCCTCAATTGCACACCTGAGCAGATCATCATAGTCAATGGAACTCAGCAGG
CCATTAACCTCACGGCGCAAGTCCTCCTCCAACAGGGAGATGAAGTCTGGCTTGATGAAC
CTGGATATGATGGCGCTTTAGGCGCATTTCTCTCACAAAATATGCGAGTTCGTCCTGTGA
GTGTTGATGTTGAGGGTACAAATATTGCCGATGCGATACAGCGGTATCCTGCAGTGAAAC
TGGCTTTTACCGCGCCCTCACACCAATTTCCCCTCGGTGGCATGCTAAGTCTGCCACGAC
GATTGGCCTTACTCGATTGGGCCGCTGAGCAGAGTAAATGGATTTTAGAGGATGACTATA
ACGGTGAGTTTCGTTACAGCGCCCGCCCAATACAGGCATTACAAGGGCTCGATAAATACC
AAAGGGTGATTTATGCAGGCACCTTTTCCAAGATGCTACTGCCCGAATTGCGACTCGGCT
TTTTAGTAGTGCCCTCTAAACTGGTACAGGCTTTTCAGCTAGCAAAGCACTACGCCGATA
CGCACACGGCTCACCTAGAGCAAGCAACATTAGCGCTGTTTATCACCGAAGGACATTATG
CGCGGCATGTTCGCCGAGTGCGAAAAGCCTGCTTAGCGCGCCAAACGGCGCTAATCGCGG
CAATTGAGCAGTATCTGCCTGACAAATTGTCCGTTCAAACCTCAGACTCAGGTATTCATC
TGGTCTGTTGGCTAAAGGGCGATTTGACCGAAGAAAAAGTGATCAATAAATGCCGCCAAC
TCAGTTTAGGTGCACAGCCTTTGTCTCGATATTGCCAAGCGCCCTATCCAAGGCAAGCAA
TCTTACTGGGCTATGCCGCTCATACGCCTGATGAGCTGCGGGAAAGTATCAAAAAACTGG
CGCAGATCCTTTAACGCGACTTAATTAATTTGGCGGATGAGAGAAGATTTTCA

Protein sequence predicted based on nucleotide sequence

MKKPQSNFPHLQLKDGILKDQLYHAFREAILDGRLHPRMKLPSSRALSETMQISRNSVLQ
GFERLIDEGYLLTKPGSGTYVSDTIPDELIDIPNSSRESAQAFDSNVDINPQLIEMARLK
AKGNTDHSHSMLNIGVGCVDLFPHQLWGRLLGRVWRQSRKTLGKSNHSAGYLPLREAISD
YARSTRGLNCTPEQIIIVNGTQQAINLTAQVLLQQGDEVWLDEPGYDGALGAFLSQNMRV
RPVSVDVEGTNIADAIQRYPAVKLAFTAPSHQFPLGGMLSLPRRLALLDWAAEQSKWILE
DDYNGEFRYSARPIQALQGLDKYQRVIYAGTFSKMLLPELRLGFLVVPSKLVQAFQLAKH
YADTHTAHLEQATLALFITEGHYARHVRRVRKACLARQTALIAAIEQYLPDKLSVQTSDS
GIHLVCWLKGDLTEEKVINKCRQLSLGAQPLSRYCQAPYPRQAILLGYAAHTPDELRESI
KKLAQIL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in 391471
Recommended expression in:
Not Applicable