PSPTO_1246 (P. syringae pv. tomato str. DC3000 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PSPTO_1246
Gene Name:  PhoH-like protein
Original Clone ID:
APC63590_SG185_pMCSG19
Keyword:
full length
Species:
Pseudomonas syringae pv. tomato str. DC3000
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Jessica Bearden
PSI
Midwest Center for Structural Genomics
PsCD00384528
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1395nts
Open reading frame: 35 to 1429

GTATGGGTACGAAACCTGTACTTCCATCCATGCCATGGATGATCACGGACGCACCCCTTC
CACCAACCAGCCAATCCTTTACGTGCTCGATACCAACGTACTGATTCACGATCCAAACGC
GTTGTTGAATTTCGAAGAACACCATGTCGCCATTCCCATGACGGTTCTGGAGGAACTGGA
CAAACTCAAGGCCGGTAAACATTCGGTGGCCGCCGAATGCCGTCAGGCCATCCGTCTGAT
CGACAAGACTCTGGGAGAAGCCAGCCCCGAAGAAGTCGAAAAGGGCGTGCCTATTGATCG
CGGGACTGGTGTGTTCAAAGGTTTCCTGTCCATTCTGATGAGCAAGCGCGAGGAACCCAA
CAGCCTGCTGCCCGAGCACTTGAACGACAACATCATCATCAACCAATTGATCGACCTGCA
CGCGCGCAACAAGGACTTGAGCGTCGTACTGGTGACCAAAGACATCAACATGCGCCTTAA
GGCGCGTGCCTGTGGCATCGACGCCGAGGATTACAGTACCGACCAGCTGGTGGACGACGT
TTCACTGCTGTCGCGTGGCTACCATGACATGACCGGCTCGTTCTGGGACCGCGTCAACAA
GGTCGATACCCGCCAGGAGCGTGGCCGTACCTGGCACCGCGTTCAACTCAATGAAATGCT
TCCGGCCGTCCATATCAACGAGTTCATTGTCGACGAACAAGGTTTTGTGGGCTGGATCAA
GGGCGTCAAGAACGACGAGTTGCTGATCCTCGACATGCATCAGGAGCCCTTGCTGCATCA
GGAAGCCTGGGGCTTGAAGCCGCGGGATATCTATCAGGGCCTGGCATTGTTCGCCTTGCT
CGACCCGGATATTCATCTGGTCAACCTGTCGGGGCCTGCGGGCTCGGGTAAAACCATCCT
GGCGCTGGCCGCTGCCATCGAGCAGACCATGGTCACCAAACGCTACCGTCGCATCATCGC
GACCCGCAGCGTTCAGGGGCTGGATCAGGAAATCGGCTTTCTGCCCGGCACCGAGGCGGA
AAAGATGGAGCCGTGGCTTGGCGCGATCACCGATAACCTCGAAGCCCTGCACATGGATGA
TGAAAACACTCACGGCAGTGTCGATTACATCCTCAGCAAAGTGCCATTGCAGTTCAAATC
CCTTAACTACATTCGAGGTCGCAGCTTCCAGCAAAGCCTGATTCTGATCGATGAATGCCA
GAACCTGACCCCGCACCAGATGAAAACCATCATCACCCGTGCCGGTGCCGGTTCCAAAGT
GGTCTGCCTGGGCAACCTGGCGCAGATCGACACCCCTTATCTGTCAGCCACCAGTTCTGG
TCTGACCTACCTGACGGAGCGCTTCAAAGACTTCCCGAACGGCGTGCACCTCACCCTGCA
AGGCGTGCCACGCTCCATTCTGGCCGAATACGCAGAGAGTCATCTTTAACATTGGAAGTG
GATAACGGATCCGAATTCGAGCTCCGTCGA

Protein sequence predicted based on nucleotide sequence

MDDHGRTPSTNQPILYVLDTNVLIHDPNALLNFEEHHVAIPMTVLEELDKLKAGKHSVAA
ECRQAIRLIDKTLGEASPEEVEKGVPIDRGTGVFKGFLSILMSKREEPNSLLPEHLNDNI
IINQLIDLHARNKDLSVVLVTKDINMRLKARACGIDAEDYSTDQLVDDVSLLSRGYHDMT
GSFWDRVNKVDTRQERGRTWHRVQLNEMLPAVHINEFIVDEQGFVGWIKGVKNDELLILD
MHQEPLLHQEAWGLKPRDIYQGLALFALLDPDIHLVNLSGPAGSGKTILALAAAIEQTMV
TKRYRRIIATRSVQGLDQEIGFLPGTEAEKMEPWLGAITDNLEALHMDDENTHGSVDYIL
SKVPLQFKSLNYIRGRSFQQSLILIDECQNLTPHQMKTIITRAGAGSKVVCLGNLAQIDT
PYLSATSSGLTYLTERFKDFPNGVHLTLQGVPRSILAEYAESHL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in APC63590
Recommended expression in:
Not Applicable