BB0479 (B. bronchiseptica )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BB0479
Gene Name:  hypothetical protein
Original Clone ID:
BoR203-21.1
Keyword:
None
Species:
Bordetella bronchiseptica
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
BbCD00384908
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1008nts
Open reading frame: 32 to 1039

ATTTTGTTTAACTTTAGAAGGAGATATACATATGACAGCCCCGGGCAGCATGCCCCTGAC
GGTGATCGGGGGTTTTCTGGGCGCCGGCAAGACCACTTTGCTCAACTACCTTCTGAAGGT
CCAGCATAGCGAGCGTGTGCTCGTCATGGTCAATGACTTCGGCTCGATCAATATCGACGC
GGAACTCCTGGATCAGGGCCGGCATGACCGCCCCGGCGTAGTCCGCCTGCAGAACGGCTG
CGTCTGCTGCTCCATTGGCGGAGACTTGATGCAGGCATTCCTGCAAGTGATGTCAATGCC
CGACAGGCCAGACCGCATCATCGTCGAAGCGAGCGGCGTTGCCGACCCCACGCGGATCGC
GCAGATAGGCCGGGTAAGCGGCGGACTCAGCGATGAAGGCGTGATCACGCTGGTGGATGC
GTCGGCTGTACGGACCCTGGCCGCCGACAAGTATGTCGGCGATATCGTCCGCCAGCAGAT
TCTGAGCGCGGACATTGTGCTGCTCAACAAGCTGGATCTCTCCAGCGACGCCGAAATCGT
CGAGACCAGGGCATGGCTGCGCGACCTTGCGCCGCGCACGCGCGTGGTTTCCTGTATCCA
CGGCGAAGTCGATGCGGTCTCGGTCCTGGGTATCGGCGCCGGCAAGGTTGCGCCGGACGC
CGCCGGCGGCCCTGTCGATGTCCCCGTGCCCGATCTGCGCCGCGCTTCGACGCGGCACGC
CGATACGTTCCATAGCTCGACGCTTGCAACGGCGCATCGCTTCGATCGGCGCAGGATCGA
GTATGCCCTCGACGCGCTTCCGCAGACGGTTTTGCGAGGCAAGGGATTTGTGGAGCTCGA
AGGCGAGGCAACGCCCGCATTGCTGCAGCTTTGCGGAAGCAGATGGACCCTGGACCCAGT
TTCCACACCGGCTCCGCCTGCCGGCACACATCTCGTTTTCATCGCGACAGGGGGCAGGGG
CGTGCCACGGTTCGATGCCCCGTCGCACTTCGAGCAGGCGTTGGCACCGCGTGACGCCGC
TTCCGCGGACACAAGCAATCTCGAGCACCACCACCACCACCACTGAGATCCGG

Protein sequence predicted based on nucleotide sequence

MTAPGSMPLTVIGGFLGAGKTTLLNYLLKVQHSERVLVMVNDFGSINIDAELLDQGRHDR
PGVVRLQNGCVCCSIGGDLMQAFLQVMSMPDRPDRIIVEASGVADPTRIAQIGRVSGGLS
DEGVITLVDASAVRTLAADKYVGDIVRQQILSADIVLLNKLDLSSDAEIVETRAWLRDLA
PRTRVVSCIHGEVDAVSVLGIGAGKVAPDAAGGPVDVPVPDLRRASTRHADTFHSSTLAT
AHRFDRRRIEYALDALPQTVLRGKGFVELEGEATPALLQLCGSRWTLDPVSTPAPPAGTH
LVFIATGGRGVPRFDAPSHFEQALAPRDAASADTSN

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t976c,S326P
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in BoR203
Recommended expression in:
Not Applicable