ERN1 (Homo sapiens) in pJP1520 (retroviral expression vector)

Explanation of Terms

Gene:
Gene Symbol:  ERN1
Gene Name:  endoplasmic reticulum to nucleus signaling 1
Original Clone ID:
FLH205466.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJP1520
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HsCD00038551
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2934nts
Open reading frame: 1 to 2934

ATGCCGGCCCGGCGGCTGCTGCTGCTGCTGACGCTGCTGCTGCCCGGCCTCGGGATTTTT
GGAAGTACCAGCACAGTGACGCTTCCTGAAACCTTGTTGTTTGTGTCAACGCTGGATGGA
AGTTTGCATGCTGTCAGCAAGAGGACAGGCTCAATCAAATGGACTTTAAAAGAAGATCCA
GTCCTGCAGGTCCCAACACATGTGGAAGAGCCTGCCTTTCTCCCAGATCCTAATGATGGC
AGCCTGTATACGCTTGGAAGCAAGAATAATGAAGGCCTGACGAAACTTCCTTTTACCATC
CCAGAATTGGTGCAGGCATCCCCATGCCGAAGTTCAGATGGAATCCTCTACATGGGTAAA
AAGCAGGACATCTGGTATGTTATTGACCTCCTGACCGGAGAGAAGCAGCAGACTTTGTCA
TCGGCCTTTGCAGATAGTCTCTGCCCATCAACCTCTCTTCTGTATCTTGGGCGAACAGAA
TACACCATCACCATGTACGACACCAAAACCCGAGAGCTCCGGTGGAATGCCACCTACTTT
GACTATGCGGCCTCACTGCCTGAGGACGACGTGGACTACAAGATGTCCCACTTTGTGTCC
AATGGTGATGGGCTGGTGGTGACTGTGGACAGTGAATCTGGGGACGTCCTGTGGATCCAA
AACTACGCCTCCCCTGTGGTGGCCTTTTATGTCTGGCAGCGGGAGGGTCTGAGGAAGGTG
ATGCACATCAATGTCGCTGTGGAGACCCTGCGCTATCTGACCTTCATGTCTGGGGAGGTG
GGGCGCATCACAAAGTGGAAGTACCCGTTCCCCAAGGAGACAGAGGCCAAGAGCAAGCTG
ACGCCCACTCTGTATGTTGGGAAATACTCTACCAGCCTCTATGCCTCTCCCTCAATGGTA
CACGAGGGGGTTGCTGTCGTGCCCCGCGGCAGCACACTTCCTTTGCTGGAAGGGCCCCAG
ACTGATGGCGTCACCATCGGGGACAAGGGGGAGTGTGTGATCACGCCCAGCACGGACGTC
AAGTTTGATCCCGGACTCAAAAGCAAGAACAAGCTCAACTACTTGAGGAATTACTGGCTT
CTGATAGGACACCATGAAACCCCACTGTCTGCGTCTACCAAGATGCTGGAGAGATTTCCC
AACAATCTACCCAAACATCGGGAAAATGTGATTCCTGCTGATTCAGAGAAAAAGAGCTTT
GAGGAAGTTATCAACCTGGTTGACCAGACTTCAGAAAACGCACCTACCACCGTGTCTCGG
GATGTGGAGGAGAAGCCCGCCCATGCCCCTGCCCGGCCCGAGGCCCCCGTGGACTCCATG
CTTAAGGACATGGCTACCATCATCCTGAGCACCTTCCTGCTGATTGGCTGGGTGGCCTTC
ATCATCACCTATCCCCTGAGCATGCATCAGCAGCAGCAGCTCCAGCACCAGCAGTTCCAG
AAGGAACTGGAGAAGATCCAGCTCCTGCAGCAGCAGCAGCAGCAGCTGCCCTTCCACCCA
CCTGGAGACACGGCTCAGGACGGCGAGCTCCTGGACACGTCTGGCCCGTACTCAGAGAGC
TCGGGCACCAGCAGCCCCAGCACGTCCCCCAGGGCCTCCAACCACTCGCTCTGCTCCGGC
AGCTCTGCCTCCAAGGCTGGCAGCAGCCCCTCCCTGGAACAAGACGATGGAGATGAGGAA
ACCAGCGTGGTGATAGTTGGGAAAATTTCCTTCTGTCCCAAGGATGTCCTGGGCCATGGA
GCTGAGGGCACAATTGTGTACCGGGGCATGTTTGACAACCGCGACGTGGCCGTGAAGAGG
ATCCTCCCCGAGTGTTTTAGCTTCGCAGACCGTGAGGTCCAGCTGTTGCGAGAATCGGAT
GAGCACCCGAACGTGATCCGCTACTTCTGCACGGAGAAGGACCGGCAATTCCAGTACATT
GCCATCGAGCTGTGTGCAGCCACCCTGCAAGAGTATGTGGAGCAGAAGGACTTTGCGCAT
CTCGGCCTGGAGCCCATCACCTTGCTGCAGCAGACCACCTCGGGCCTGGCCCACCTCCAC
TCCCTCAACATCGTTCACAGAGACCTAAAGCCACACAACATCCTCATATCCATGCCCAAT
GCACACGGCAAGATCAAGGCCATGATCTCCGACTTTGGCCTCTGCAAGAAGCTGGCAGTG
GGCAGACACAGTTTCAGCCGCCGATCTGGGGTGCCTGGCACAGAAGGCTGGATCGCTCCA
GAGATGCTGAGCGAAGACTGTAAGGAGAACCCTACCTACACGGTGGACATCTTTTCTGCA
GGCTGCGTCTTTTACTACGTAATCTCTGAGGGCAGCCACCCTTTTGGCAAGTCCCTGCAG
CGGCAGGCCAACATCCTCCTGGGTGCCTGCAGCCTTGACTGCTTGCACCCAGAGAAGCAC
GAAGACGTCATTGCACGTGAATTGATAGAGAAGATGATTGCGATGGATCCTCAGAAACGC
CCCTCAGCGAAGCACGTGCTCAAACACCCGTTCTTCTGGAGCCTAGAGAAGCAGCTCCAG
TTCTTCCAGGACGTGAGCGACAGAATAGAAAAGGAATCCCTGGATGGCCCGATCGTGAAG
CAGTTAGAGAGAGGCGGGAGAGCCGTGGTGAAGATGGACTGGCGGGAGAACATCACTGTC
CCCCTCCAGACAGACCTGCGTAAATTCAGGACCTATAAAGGTGGTTCTGTCAGAGATCTC
CTCCGAGCCATGAGAAATAAGAAGCACCACTACCGGGAGCTGCCTGCAGAGGTGCGGGAG
ACGCTGGGGTCCCTCCCCGACGACTTCGTGTGCTACTTCACGTCTCGCTTCCCCCACCTC
CTCGCACACACCTACCGGGCCATGGAGCTGTGCAGCCACGAGAGACTCTTCCAGCCCTAC
TACTTCCACGAGCCCCCAGAGCCCCAGCCCCCAGTGACTCCAGACACCCTCTTG

Protein sequence predicted based on nucleotide sequence

MPARRLLLLLTLLLPGLGIFGSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDP
VLQVPTHVEEPAFLPDPNDGSLYTLGSKNNEGLTKLPFTIPELVQASPCRSSDGILYMGK
KQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYF
DYAASLPEDDVDYKMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQREGLRKV
MHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMV
HEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWL
LIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSR
DVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQ
KELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHSLCSG
SSASKAGSSPSLEQDDGDEETSVVIVGKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKR
ILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAH
LGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILISMPNAHGKIKAMISDFGLCKKLAV
GRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQ
RQANILLGACSLDCLHPEKHEDVIARELIEKMIAMDPQKRPSAKHVLKHPFFWSLEKQLQ
FFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDL
LRAMRNKKHHYRELPAEVRETLGSLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPY
YFHEPPEPQPPVTPDTLL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 3030
Start on reference sequence 97
Mutations:
None
Discrepancy:
a570c,E190D; g572t,G191V;
 g2301a; g2302a,V768I;
 a2418t; c2472g,N824K;
 g2473c,D825H; a2639t,D880V;
 a2770t,T924S; g2926a,A976T
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable