MCA0842 (M. capsulatus str. Bath )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MCA0842
Gene Name:  diguanylate cyclase
Original Clone ID:
McR177D-21.5
Keyword:
targeted domain
Species:
Methylococcus capsulatus str. Bath
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
McCD00385536
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 537nts
Open reading frame: 51 to 587

AATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGGCGACG
GCGCCCGAGCCTTCAACCAACTGATCCAGGCACTGTCACTTTCGATCGAAACCGAAACGG
CGGTCCGCACGTTCACCGAGATGCTGGTCAGCGAACTCGACGTGCATCAGCTCTCCGAGC
AGGCTTTGGCGCTCCTGCTGCAACATACCGGTGCCGACGGCGGTGCGATCCTGATCGAAG
TGGACGGCGAAATCCGGGTTGCTGCGTCGCAGGGCATCGGCAGCGCCCACACCCTGGCGA
ACAACGACCGCGTCCGCAGCGCGCTGCGCACCGAGCGCCGCCACAGGGTGCAACTGCCCG
AGGATCTCGTGGTAGACGGCATGCTGGCGCAGTTCCGGCCGAAGGAGGTCATCATCGATC
CCGTGCGGCACAAGGAAGTGGTGCTGGGAGCGATCGTGTTGGCCAGCGGCCAAGGCTTCA
GCGCCGAAGACCGGAATCGCCTGGACGTGTTCGAGAGAGGCCTCGCGTTGGCGTTGCACA
ACGCGCTCCTGTACGACCGTCTGCAGACGCTGGCGGCACTCGATCCCCTCGAGCACCACC
ACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGA

Protein sequence predicted based on nucleotide sequence

MGDGARAFNQLIQALSLSIETETAVRTFTEMLVSELDVHQLSEQALALLLQHTGADGGAI
LIEVDGEIRVAASQGIGSAHTLANNDRVRSALRTERRHRVQLPEDLVVDGMLAQFRPKEV
IIDPVRHKEVVLGAIVLASGQGFSAEDRNRLDVFERGLALALHNALLYDRLQTLAALDP

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a124t,T42S;g174t,G58G;
t258c,D86D;a346c,R116R;
g403a,V135I;a483g,A161A;
t507c,D169D
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in McR177D
Recommended expression in:
Not Applicable