Maqu_2914 (M. nauticus )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Maqu_2914
Gene Name:  PAS/PAC sensor-containing diguanylate cyclase
Original Clone ID:
MqR66B-21.1
Keyword:
targeted domain
Species:
Marinobacter nauticus
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
MaCD00385571
Price: 
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 396nts
Open reading frame: 51 to 446

AATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCCTGAAC
AATCCATTGAAATGACGCCGGACAGCTCTGTCTACAAGGCACTGCTGGAATCCACAAAGG
CGATTCCATGGAAAATCAACTGGCAGACCATGGCATTCGAATACATCGGTCCGCAGATAG
AAGCATTGCTGGGCTGGCCCCAGGGTAGCTGGAAAAGTGTCGAAGACTGGGCCACCCGTA
TGCATCCGGAGGACCAGGAGTGGGTCGTCAATTTCTGCGTCAAACAATCGGAGTGCGGCG
TCGATCATGTAGCAGACTACCGCGCTCTGCATCGGGACGGTCACTATGTCTGGATACGTG
ACGTCGTGCAAGTTGTTCGCGATGACAGTGGCGAAGTAGAAGCCCTGATCGGCTTCATGT
TCGACATCAGCGAACGCAAGAAAACCCTCGAGCACCACCACCACCACCACTGAGATCCGG
CTGCTAACAAAGCCCGA

Protein sequence predicted based on nucleotide sequence

MPEQSIEMTPDSSVYKALLESTKAIPWKINWQTMAFEYIGPQIEALLGWPQGSWKSVEDW
ATRMHPEDQEWVVNFCVKQSECGVDHVADYRALHRDGHYVWIRDVVQVVRDDSGEVEALI
GFMFDISERKKT

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a260t,E87V;t321a,H107Q
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in MqR66B
Recommended expression in:
Not Applicable