MCA0337 (M. capsulatus str. Bath )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MCA0337
Gene Name:  diguanylate phosphodiesterase
Original Clone ID:
McR174B-21.6
Keyword:
full length protein truncation
Species:
Methylococcus capsulatus str. Bath
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
McCD00385579
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 747nts
Open reading frame: 48 to 794

TCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCTGAACGTCC
AGGCCTACCGGCGGCAGGAAATCGAAAGGAACCTCCGCTGCGCCCTGCTCCAGGGCGAAC
TCGGCCTGAGCTATGCGCCCCGGGTGGATTTGCGCAGCGGCAGGATCGTCGCCCTGGAGG
TGTGTCCCTTCTGGCTCAGTTCCGAGCTGGGAGACGTGCCGCCGGACGACTTCGTTCCCG
TGGCGGAGGAGACCGGTCTCATCGTGCAGATCGGTATCTGGGCTTTTCGCACCGCCTGCG
CTCATGCGGCGGTGTGGCGGACGCAAGGACTCGACGACCGCTGTCTCTCTTTCCGGTTGT
CGCAGCGGGAACTGGATGATCCCCAGTTCGGCGATAGGATCATGAGTTTGCTGGATGGCT
GTGGTCTGGAACCGGAGAACCTGCAGTTCGAACTCTCGGAGCGCATGCTGATGACGCATG
CCGCACACACCCTCGCCGTGCTGCAGGAACTCAGCAACCGGGGCATCCGCTTCTCGGTGG
GGGACTTCGGCACGGGCAATGCGTCCTTGCGGCGGCTGCGGCGGTTTCCGATCGATGAAC
TCAAGATCGATCTGTCATCCGTCCGGGACATCGAGCCGGATTCGGACGATGCCGCCGTCA
TCACCGCGATGATTGCCATGACCAAGAGTCTGCGGATGGACGTCGTCGCCGTCGGCGTGG
AAACCCCGGAGCACGTGGCATTCCTGCTTGAGTGCGGGTGTCGTCACGGGCAGGGCCGTC
TGATCGGGGCGCGGCTCGAGCACCACCACCACCACCACTGAGATCCG

Protein sequence predicted based on nucleotide sequence

MLNVQAYRRQEIERNLRCALLQGELGLSYAPRVDLRSGRIVALEVCPFWLSSELGDVPPD
DFVPVAEETGLIVQIGIWAFRTACAHAAVWRTQGLDDRCLSFRLSQRELDDPQFGDRIMS
LLDGCGLEPENLQFELSERMLMTHAAHTLAVLQELSNRGIRFSVGDFGTGNASLRRLRRF
PIDELKIDLSSVRDIEPDSDDAAVITAMIAMTKSLRMDVVAVGVETPEHVAFLLECGCRH
GQGRLIGAR

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c82a,R28S;a159g,E53E;
a187g,I63V;a204g,E68E;
c282a,G94G;c287a,A96D;
c411g,S137S;g634a,A212T;
c706t,R236C
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in McR174B
Recommended expression in:
Not Applicable