PA5048 (P. aeruginosa )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PA5048
Gene Name:  nuclease
Original Clone ID:
PaR358-21.2
Keyword:
None
Species:
Pseudomonas aeruginosa
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
PaCD00385632
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 765nts
Open reading frame: 31 to 795

ATTTTGTTTACTTTAGAAGGAGATATACATATGGGCGCCTTTTTCATGTCCGTGGTTTCC
GCCTTCGCCGGTGCGGCGGAGGCCTGCTGGCCTACCGGCGCCTTGCAGAACGTCGAGGTG
GCGCGGGTGGTGGACGGCGATACCGTGCGCCTGCGCGATGGCCGCAGCGTGCGGCTGATC
GGTATCAACGCGCCTGAGCTGGCGCACAACGGTCGCACCACCGAGCCCTTCGCCGAAGCC
GCGAAGCAGCGACTGCAGGCGCTGGTATCGGCCAGCGACGGGCGTCTCGCCCTGCAGCCG
GGCCGCCAGGCCCGCGATCACTACGGGCGGACCCTGGCGCACCTGTTCGATGCCAGCGGC
GCCAACCTCGAGGCGCGCCTGCTCGGCGAGGGCCTGGGCTACCTGGTGGCGGTCGCACCG
AACACCGAGCTGGCGGCCTGTCAGCAAGCGGCGGAACGTGCAGCGCGTTCGGCTAATCTG
GGTGTATGGAAGCGTTCGCCGGTGCTTTCGCTCGATCGACTGGCGCAGAGCGGCTTCGCC
GTGGTCCGCGGGCGGGTGCTCGAGGTCGAACGCAACCGGGGCGGTATCTGGCTGGAGATG
GACGGTCCGCTGGTCCTGCGGATCGAGCCGAAGGTGCTCAAGGTGTTCGATGCGAAACGG
GTGATGCAGATGGCGGGACGTTCGCTGGAAGTGCGTGGCTGGGTGGTCGATCGTTCCCGC
CGTGGCGCTGTGCCGGCCGGCAAGGCGCGCTGGCAGATGTCGGTGACCGATCCGGCGATG
CTGGAATGGTTGCCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAA
GCCCGA

Protein sequence predicted based on nucleotide sequence

MGAFFMSVVSAFAGAAEACWPTGALQNVEVARVVDGDTVRLRDGRSVRLIGINAPELAHN
GRTTEPFAEAAKQRLQALVSASDGRLALQPGRQARDHYGRTLAHLFDASGANLEARLLGE
GLGYLVAVAPNTELAACQQAAERAARSANLGVWKRSPVLSLDRLAQSGFAVVRGRVLEVE
RNRGGIWLEMDGPLVLRIEPKVLKVFDAKRVMQMAGRSLEVRGWVVDRSRRGAVPAGKAR
WQMSVTDPAMLEWLP

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c58t,R20W;g387a,A129A;
a403g,T135A;c614t,A205V
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in PaR358
Recommended expression in:
Not Applicable