DNASU Plasmid | STIP1 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: STIP1

Gene Name: stress-induced phosphoprotein 1

Original Clone ID:

HR4403D-327-543-14.11

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Northeast Structural Genomics Consortium

HsCD00385688

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 657nts

Open reading frame: 51 to 707

Reference Sequence Alignment

CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGCTGGCAG
AGCACCGAACCCCAGATGTGCTCAAGAAATGCCAGCAGGCAGAGAAAATCCTGAAGGAGC
AAGAGCGGCTGGCCTACATAAACCCCGACCTGACTTTGGAGGAGAAGAACGAAGGCAACG
AGTGTTTTCAGAAAGGGGACTATCCCCAGGCCATGAAGCATTATACAGAAGCCATCAAAA
GGAACCCGAAAGATGCCAAATTATACAGCAATCGAGCTGCCTGCTACACCAAACTCCTGG
AGTTCCAGCTGGCACTCAAGGACTGTGAGGAATGTATCCAGCTGGAGCCGACCTTCATCA
AGGGTTATACACGGAAAGCCGCTGCGCTGGAAGCGATGAAGGACTACACCAAAGCCATGG
ATGTGTACCAGAAGGCGCTAGACCTGGACTCCAGCTGTAAGGAGGCGGCAGACGGCTACC
AGCGCTGTATGATGGCGCAGTACAACCGGCACGACAGCCCCGAAGATGTGAAGCGACGAG
CCATGGCCGACCCTGAGGTGCAGCAGATCATGAGTGACCCAGCCATGCGCCTTATCCTGG
AACAGATGCAGAAGGACCCCCAGGCACTCAGCGAACACTTAAAGAATCCTGTAATAGCAC
AGAAGATCCAGAAGCTGATGGATGTGGGTCTGATTGCAATTCGGTAGCTCGAGATCCGGC
TGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG

Protein sequence predicted based on nucleotide sequence

MLAEHRTPDVLKKCQQAEKILKEQERLAYINPDLTLEEKNEGNECFQKGDYPQAMKHYTE
AIKRNPKDAKLYSNRAACYTKLLEFQLALKDCEECIQLEPTFIKGYTRKAAALEAMKDYT
KAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMR
LILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

g103a,A35T;a121g,K41E

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

Find experimental protocols in HR4403D

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

STIP1

Symbol Nomenclature :

STIP1

SYNONYM :

HEL-S-94n; HOP; IEF-SSP-3521; P60; STI1; STI1L

Designation :

Full Nomenclature :

stress-induced phosphoprotein 1

GENEID :

10963

GI :

5803181

GenBank Accession :

NM_006819

HGNC :

11387

MIM :

605063

Vega :

OTTHUMG00000167789

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00385688        ------------------------------------------------------------
NM_006819.2         ------------------------------------------------------------
NM_001282653.1      ------------------------------------------------------------
NM_001282652.1      ATGGAGTCCGGCAGCCCAATGGGAGAGGTGGAAATTTCCAGAACGATCAGAACCAATGGG
                                                                                

HsCD00385688        ------------------------------------------------------------
NM_006819.2         ------------------------------------------------------------
NM_001282653.1      ------------------------------------------------------------
NM_001282652.1      CGCGGCCAGCGCGGCTACGATTGGCAGTGCAAAAGACCAATCCGTGTCGCAGAAGTTCGC
                                                                                

HsCD00385688        ------------------------------------------------------------
NM_006819.2         ---------------------ATGGAGCAGGTCAATGAGCTGAAGGAGAAAGGCAACAAG
NM_001282653.1      ---------------------ATGGAGCAGGTCAATGAGCTGAAGGAGAAAGGCAACAAG
NM_001282652.1      TCCTCCCTCCATTCGTGGAGCCTGAGATGGGTCAATGAGCTGAAGGAGAAAGGCAACAAG
                                                                                

HsCD00385688        ------------------------------------------------------------
NM_006819.2         GCCCTGAGCGTGGGTAACATCGATGATGCCTTACAGTGCTACTCCGAAGCTATTAAGCTG
NM_001282653.1      GCCCTGAGCGTGGGTAACATCGATGATGCCTTACAGTGCTACTCCGAAGCTATTAAGCTG
NM_001282652.1      GCCCTGAGCGTGGGTAACATCGATGATGCCTTACAGTGCTACTCCGAAGCTATTAAGCTG
                                                                                

HsCD00385688        ------------------------------------------------------------
NM_006819.2         GATCCCCACAACCACGTGCTGTACAGCAACCGTTCTGCTGCCTATGCCAAGAAAGGAGAC
NM_001282653.1      GATCCCCACAACCACGTGCTGTACAGCAACCGTTCTGCTGCCTATGCCAAGAAAGGAGAC
NM_001282652.1      GATCCCCACAACCACGTGCTGTACAGCAACCGTTCTGCTGCCTATGCCAAGAAAGGAGAC
                                                                                

HsCD00385688        ------------------------------------------------------------
NM_006819.2         TACCAGAAGGCTTATGAGGATGGCTGCAAGACTGTCGACCTAAAGCCTGACTGGGGCAAG
NM_001282653.1      TACCAGAAGGCTTATGAGGATGGCTGCAAGACTGTCGACCTAAAGCCTGACTGGGGCAAG
NM_001282652.1      TACCAGAAGGCTTATGAGGATGGCTGCAAGACTGTCGACCTAAAGCCTGACTGGGGCAAG
                                                                                

HsCD00385688        ------------------------------------------------------------
NM_006819.2         GGCTATTCACGAAAAGCAGCAGCTCTAGAGTTCTTAAACCGCTTTGAAGAAGCCAAGCGA
NM_001282653.1      G-----------------------------------------------------------
NM_001282652.1      GGCTATTCACGAAAAGCAGCAGCTCTAGAGTTCTTAAACCGCTTTGAAGAAGCCAAGCGA
                                                                                

HsCD00385688        ------------------------------------------------------------
NM_006819.2         ACCTATGAGGAGGGCTTAAAACACGAGGCAAATAACCCTCAACTGAAAGAGGGTTTACAG
NM_001282653.1      -------------GCTTAAAACACGAGGCAAATAACCCTCAACTGAAAGAGGGTTTACAG
NM_001282652.1      ACCTATGAGGAGGGCTTAAAACACGAGGCAAATAACCCTCAACTGAAAGAGGGTTTACAG
                                                                                

HsCD00385688        ------------------------------------------------------------
NM_006819.2         AATATGGAGGCCAGGTTGGCAGAGAGAAAATTCATGAACCCTTTCAACATGCCTAATCTG
NM_001282653.1      AATATGGAGGCCAGGTTGGCAGAGAGAAAATTCATGAACCCTTTCAACATGCCTAATCTG
NM_001282652.1      AATATGGAGGCCAGGTTGGCAGAGAGAAAATTCATGAACCCTTTCAACATGCCTAATCTG
                                                                                

HsCD00385688        ------------------------------------------------------------
NM_006819.2         TATCAGAAGTTGGAGAGTGATCCCAGGACAAGGACACTACTCAGTGATCCTACCTACCGG
NM_001282653.1      TATCAGAAGTTGGAGAGTGATCCCAGGACAAGGACACTACTCAGTGATCCTACCTACCGG
NM_001282652.1      TATCAGAAGTTGGAGAGTGATCCCAGGACAAGGACACTACTCAGTGATCCTACCTACCGG
                                                                                

HsCD00385688        ------------------------------------------------------------
NM_006819.2         GAGCTGATAGAGCAGCTACGAAACAAGCCTTCTGACCTGGGCACGAAACTACAAGATCCC
NM_001282653.1      GAGCTGATAGAGCAGCTACGAAACAAGCCTTCTGACCTGGGCACGAAACTACAAGATCCC
NM_001282652.1      GAGCTGATAGAGCAGCTACGAAACAAGCCTTCTGACCTGGGCACGAAACTACAAGATCCC
                                                                                

HsCD00385688        ------------------------------------------------------------
NM_006819.2         CGGATCATGACCACTCTCAGCGTCCTCCTTGGGGTCGATCTGGGCAGTATGGATGAGGAG
NM_001282653.1      CGGATCATGACCACTCTCAGCGTCCTCCTTGGGGTCGATCTGGGCAGTATGGATGAGGAG
NM_001282652.1      CGGATCATGACCACTCTCAGCGTCCTCCTTGGGGTCGATCTGGGCAGTATGGATGAGGAG
                                                                                

HsCD00385688        ------------------------------------------------------------
NM_006819.2         GAAGAGATTGCAACACCTCCACCACCACCCCCTCCCAAAAAGGAGACCAAGCCAGAGCCA
NM_001282653.1      GAAGAGATTGCAACACCTCCACCACCACCCCCTCCCAAAAAGGAGACCAAGCCAGAGCCA
NM_001282652.1      GAAGAGATTGCAACACCTCCACCACCACCCCCTCCCAAAAAGGAGACCAAGCCAGAGCCA
                                                                                

HsCD00385688        ------------------------------------------------------------
NM_006819.2         ATGGAAGAAGATCTTCCAGAGAATAAGAAGCAGGCACTGAAAGAAAAAGAGCTGGGGAAC
NM_001282653.1      ATGGAAGAAGATCTTCCAGAGAATAAGAAGCAGGCACTGAAAGAAAAAGAGCTGGGGAAC
NM_001282652.1      ATGGAAGAAGATCTTCCAGAGAATAAGAAGCAGGCACTGAAAGAAAAAGAGCTGGGGAAC
                                                                                

HsCD00385688        ------------------------------------------------------------
NM_006819.2         GATGCCTACAAGAAGAAAGACTTTGACACAGCCTTGAAGCATTACGACAAAGCCAAGGAG
NM_001282653.1      GATGCCTACAAGAAGAAAGACTTTGACACAGCCTTGAAGCATTACGACAAAGCCAAGGAG
NM_001282652.1      GATGCCTACAAGAAGAAAGACTTTGACACAGCCTTGAAGCATTACGACAAAGCCAAGGAG
                                                                                

HsCD00385688        ------------------------------------------------------------
NM_006819.2         CTGGACCCCACTAACATGACTTACATTACCAATCAAGCAGCGGTATACTTTGAAAAGGGC
NM_001282653.1      CTGGACCCCACTAACATGACTTACATTACCAATCAAGCAGCGGTATACTTTGAAAAGGGC
NM_001282652.1      CTGGACCCCACTAACATGACTTACATTACCAATCAAGCAGCGGTATACTTTGAAAAGGGC
                                                                                

HsCD00385688        ------------------------------------------------------------
NM_006819.2         GACTACAATAAGTGCCGGGAGCTTTGTGAGAAGGCCATTGAAGTGGGGAGAGAAAACCGA
NM_001282653.1      GACTACAATAAGTGCCGGGAGCTTTGTGAGAAGGCCATTGAAGTGGGGAGAGAAAACCGA
NM_001282652.1      GACTACAATAAGTGCCGGGAGCTTTGTGAGAAGGCCATTGAAGTGGGGAGAGAAAACCGA
                                                                                

HsCD00385688        ----------------------------------------------CTTTAAGAAGGAGA
NM_006819.2         GAAGACTATCGACAGATTGCCAAAGCATATGCTCGAATTGGCAACTCCTACTTCAAAGAA
NM_001282653.1      GAAGACTATCGACAGATTGCCAAAGCATATGCTCGAATTGGCAACTCCTACTTCAAAGAA
NM_001282652.1      GAAGACTATCGACAGATTGCCAAAGCATATGCTCGAATTGGCAACTCCTACTTCAAAGAA
                                                                  * *:.: .*....*

HsCD00385688        TATACCATGGGCCATCACCATCACCATCACAGCCATATGCTGGCAGAGCACCGAACCCCA
NM_006819.2         GAAAAGTACAAGGATGCCATCCATTTCTATAACAAGTCTCTGGCAGAGCACCGAACCCCA
NM_001282653.1      GAAAAGTACAAGGATGCCATCCATTTCTATAACAAGTCTCTGGCAGAGCACCGAACCCCA
NM_001282652.1      GAAAAGTACAAGGATGCCATCCATTTCTATAACAAGTCTCTGGCAGAGCACCGAACCCCA
                     *:*. :: ..  ** .*.: **  :  * *.*.* :  *********************

HsCD00385688        GATGTGCTCAAGAAATGCCAGCAGGCAGAGAAAATCCTGAAGGAGCAAGAGCGGCTGGCC
NM_006819.2         GATGTGCTCAAGAAATGCCAGCAGGCAGAGAAAATCCTGAAGGAGCAAGAGCGGCTGGCC
NM_001282653.1      GATGTGCTCAAGAAATGCCAGCAGGCAGAGAAAATCCTGAAGGAGCAAGAGCGGCTGGCC
NM_001282652.1      GATGTGCTCAAGAAATGCCAGCAGGCAGAGAAAATCCTGAAGGAGCAAGAGCGGCTGGCC
                    ************************************************************

HsCD00385688        TACATAAACCCCGACCTGACTTTGGAGGAGAAGAACGAAGGCAACGAGTGTTTTCAGAAA
NM_006819.2         TACATAAACCCCGACCTGGCTTTGGAGGAGAAGAACAAAGGCAACGAGTGTTTTCAGAAA
NM_001282653.1      TACATAAACCCCGACCTGGCTTTGGAGGAGAAGAACAAAGGCAACGAGTGTTTTCAGAAA
NM_001282652.1      TACATAAACCCCGACCTGGCTTTGGAGGAGAAGAACAAAGGCAACGAGTGTTTTCAGAAA
                    ******************.*****************.***********************

HsCD00385688        GGGGACTATCCCCAGGCCATGAAGCATTATACAGAAGCCATCAAAAGGAACCCGAAAGAT
NM_006819.2         GGGGACTATCCCCAGGCCATGAAGCATTATACAGAAGCCATCAAAAGGAACCCGAAAGAT
NM_001282653.1      GGGGACTATCCCCAGGCCATGAAGCATTATACAGAAGCCATCAAAAGGAACCCGAAAGAT
NM_001282652.1      GGGGACTATCCCCAGGCCATGAAGCATTATACAGAAGCCATCAAAAGGAACCCGAAAGAT
                    ************************************************************

HsCD00385688        GCCAAATTATACAGCAATCGAGCTGCCTGCTACACCAAACTCCTGGAGTTCCAGCTGGCA
NM_006819.2         GCCAAATTATACAGCAATCGAGCTGCCTGCTACACCAAACTCCTGGAGTTCCAGCTGGCA
NM_001282653.1      GCCAAATTATACAGCAATCGAGCTGCCTGCTACACCAAACTCCTGGAGTTCCAGCTGGCA
NM_001282652.1      GCCAAATTATACAGCAATCGAGCTGCCTGCTACACCAAACTCCTGGAGTTCCAGCTGGCA
                    ************************************************************

HsCD00385688        CTCAAGGACTGTGAGGAATGTATCCAGCTGGAGCCGACCTTCATCAAGGGTTATACACGG
NM_006819.2         CTCAAGGACTGTGAGGAATGTATCCAGCTGGAGCCGACCTTCATCAAGGGTTATACACGG
NM_001282653.1      CTCAAGGACTGTGAGGAATGTATCCAGCTGGAGCCGACCTTCATCAAGGGTTATACACGG
NM_001282652.1      CTCAAGGACTGTGAGGAATGTATCCAGCTGGAGCCGACCTTCATCAAGGGTTATACACGG
                    ************************************************************

HsCD00385688        AAAGCCGCTGCGCTGGAAGCGATGAAGGACTACACCAAAGCCATGGATGTGTACCAGAAG
NM_006819.2         AAAGCCGCTGCGCTGGAAGCGATGAAGGACTACACCAAAGCCATGGATGTGTACCAGAAG
NM_001282653.1      AAAGCCGCTGCGCTGGAAGCGATGAAGGACTACACCAAAGCCATGGATGTGTACCAGAAG
NM_001282652.1      AAAGCCGCTGCGCTGGAAGCGATGAAGGACTACACCAAAGCCATGGATGTGTACCAGAAG
                    ************************************************************

HsCD00385688        GCGCTAGACCTGGACTCCAGCTGTAAGGAGGCGGCAGACGGCTACCAGCGCTGTATGATG
NM_006819.2         GCGCTAGACCTGGACTCCAGCTGTAAGGAGGCGGCAGACGGCTACCAGCGCTGTATGATG
NM_001282653.1      GCGCTAGACCTGGACTCCAGCTGTAAGGAGGCGGCAGACGGCTACCAGCGCTGTATGATG
NM_001282652.1      GCGCTAGACCTGGACTCCAGCTGTAAGGAGGCGGCAGACGGCTACCAGCGCTGTATGATG
                    ************************************************************

HsCD00385688        GCGCAGTACAACCGGCACGACAGCCCCGAAGATGTGAAGCGACGAGCCATGGCCGACCCT
NM_006819.2         GCGCAGTACAACCGGCACGACAGCCCCGAAGATGTGAAGCGACGAGCCATGGCCGACCCT
NM_001282653.1      GCGCAGTACAACCGGCACGACAGCCCCGAAGATGTGAAGCGACGAGCCATGGCCGACCCT
NM_001282652.1      GCGCAGTACAACCGGCACGACAGCCCCGAAGATGTGAAGCGACGAGCCATGGCCGACCCT
                    ************************************************************

HsCD00385688        GAGGTGCAGCAGATCATGAGTGACCCAGCCATGCGCCTTATCCTGGAACAGATGCAGAAG
NM_006819.2         GAGGTGCAGCAGATCATGAGTGACCCAGCCATGCGCCTTATCCTGGAACAGATGCAGAAG
NM_001282653.1      GAGGTGCAGCAGATCATGAGTGACCCAGCCATGCGCCTTATCCTGGAACAGATGCAGAAG
NM_001282652.1      GAGGTGCAGCAGATCATGAGTGACCCAGCCATGCGCCTTATCCTGGAACAGATGCAGAAG
                    ************************************************************

HsCD00385688        GACCCCCAGGCACTCAGCGAACACTTAAAGAATCCTGTAATAGCACAGAAGATCCAGAAG
NM_006819.2         GACCCCCAGGCACTCAGCGAACACTTAAAGAATCCTGTAATAGCACAGAAGATCCAGAAG
NM_001282653.1      GACCCCCAGGCACTCAGCGAACACTTAAAGAATCCTGTAATAGCACAGAAGATCCAGAAG
NM_001282652.1      GACCCCCAGGCACTCAGCGAACACTTAAAGAATCCTGTAATAGCACAGAAGATCCAGAAG
                    ************************************************************

HsCD00385688        CTGATGGATGTGGGTCTGATTGCAATTCGGTAGCTCGAGATCCGGCTGCTAACAAAGCCC
NM_006819.2         CTGATGGATGTGGGTCTGATTGCAATTCGGTGA---------------------------
NM_001282653.1      CTGATGGATGTGGGTCTGATTGCAATTCGGTGA---------------------------
NM_001282652.1      CTGATGGATGTGGGTCTGATTGCAATTCGGTGA---------------------------
                    *******************************..                           

HsCD00385688        GAAAGGAAGCTGAGTTGGCTGCTG
NM_006819.2         ------------------------
NM_001282653.1      ------------------------
NM_001282652.1      ------------------------