PFL_0442 (P. fluorescens )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PFL_0442
Gene Name:  nuclease, SNase family
Original Clone ID:
PlR281-21.1
Keyword:
None
Species:
Pseudomonas fluorescens
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
PfCD00385722
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 759nts
Open reading frame: 32 to 790

ATTTTGTTTACTTTAAGAAGGAGATATACATATGGGCGCCTTTTTTGTGGGCGCGATTTG
GCTGACAGCGGCCGGGGCGTTTTGCCCGGCGCCCAAGAGGCTGTTGCCGGCAACGGTGCT
CCGGGTGGTGGACGGCGACACCCTGCACCTCAAGGATGGCCGCAGGTTGCGCATGATCGG
CCTCAATGCCCCGGAGCTGGCGGGCAAGGGGCGCCCTGCCGAGCCCTTCGCCGAGGCGGC
GCGGCAGCGCCTGCGACAACTGGTGGCACAGAGTGGTAGGCGGGTCGGCCTGCTGCCGGG
GCGTGAAAGGCAGGATCGCTACGGTCGTGTCCTGGCCCATGTCTATGGCGTCACTGGTAT
CAACTTCGAAGCCCGGTTGATCGCGGAAGGCTTGGCCTATCGGATTGCCGTGCTGCCAAA
CGTCGCCCTGGCGAGCTGCCAGAAGGCTGCCGAGAACCATGCGCGCCGAGCCGGGCTGGG
GCTTTGGCGACGTTCACCTGTACTCAAGGCGCAGCAGATCCATCGCGCGGGCTTTGCCCT
GTTCGAAGGGCGAGTGAGCAGGGTGCAGCGCAATCGCGGTGGTCTTTGGATCACATTGCA
GGGGCAGGTTGTACTGCGCATTGCACCCAATGTCCTGCGGCGCTTCGATCGCCGGCTGCT
CAGTGGCCTTTCGGGGCGCCGGATCGAGGCTCGCGGCTGGGTTGTCGATCGCTCGCGTCA
GCCTGGCCAGCAATCTGCACATCCGCGCTGGATGTTGTCCGTGACAGACCCTTCCATGCT
CGGACTTATCCTCGAGCACCACCACCACCACCACTGAGATCCGG

Protein sequence predicted based on nucleotide sequence

MGAFFVGAIWLTAAGAFCPAPKRLLPATVLRVVDGDTLHLKDGRRLRMIGLNAPELAGKG
RPAEPFAEAARQRLRQLVAQSGRRVGLLPGRERQDRYGRVLAHVYGVTGINFEARLIAEG
LAYRIAVLPNVALASCQKAAENHARRAGLGLWRRSPVLKAQQIHRAGFALFEGRVSRVQR
NRGGLWITLQGQVVLRIAPNVLRRFDRRLLSGLSGRRIEARGWVVDRSRQPGQQSAHPRW
MLSVTDPSMLGLI

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a44g,E15G;g67a,G23R;
t198c,F66F;g247a,G83R;
t583c,L195L;g698a,R233Q
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in PlR281
Recommended expression in:
Not Applicable