PFL_0442 (P. fluorescens )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PFL_0442
Gene Name:  nuclease, SNase family
Original Clone ID:
PlR281H-21.2
Keyword:
full length protein truncation
Species:
Pseudomonas fluorescens
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
PfCD00385739
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 549nts
Open reading frame: 51 to 599

AATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCGTTTT
GCCCGGCGCCCAAGGGGCTGTTGCCGGCAACGGTGCTCCGGGTGGTGGACGGCGACACCC
TGCACCTCAAGGATGGCCGCAGGTTGCGCATGATCGGCCTCAATGCCCCGGAGCTGGCGG
GCAAGGGGCGCCCTGCCGAGCCCTTTGCCGAGGCGGCGCGGCAGCGCCTGCGACAACTGG
TGGCACAGAGTGGTGGGCGGGTCGGCCTGCTGCCGGGGCGTGAAAGGCAGGATCGCTACG
GTCGTGTCTTGGCCCATGTCTATGGCGACACTGGTATCAACTTCGAAGCCCGGTTGATCG
CGGAAGGCTTGGCCTATCGGATTGCCGTGCTGCCAAACGTCGCCCTGGCGAGCTGCCAGA
AGGCTGCCGAGAACCATGCGCGCCGAGCCGGGCTGGGGCTTTGGCGACGTTCACCTGTAC
TCAAGGCGCAGCAGATCCATCGCGCGGGCTTTGCCCTGTTCGAAGGGCGAGTGAGCAGGG
TGCAGCGCAATCGCGGTGGTCTTTGGATCACATTGCAGGGGCAGGTTGTATTGCGCATTC
TCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGA

Protein sequence predicted based on nucleotide sequence

MAFCPAPKGLLPATVLRVVDGDTLHLKDGRRLRMIGLNAPELAGKGRPAEPFAEAARQRL
RQLVAQSGGRVGLLPGRERQDRYGRVLAHVYGDTGINFEARLIAEGLAYRIAVLPNVALA
SCQKAAENHARRAGLGLWRRSPVLKAQQIHRAGFALFEGRVSRVQRNRGGLWITLQGQVV
LRI

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c259t,L87L;t278a,V93D
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in PlR281H
Recommended expression in:
Not Applicable