PASK (Homo sapiens) in pJP1520 (retroviral expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PASK
Gene Name:  PAS domain containing serine/threonine kinase
Original Clone ID:
FLH205515.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJP1520
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HsCD00038595
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3972nts
Open reading frame: 1 to 3972

ATGGAGGACGGGGGCTTAACAGCCTTTGAAGAGGACCAGAGATGCCTTTCCCAGAGCCTC
CCCTTGCCAGTGTCAGCAGAGGGCCCAGCTGCACAGACCACTGCTGAGCCCAGCAGGTCG
TTTTCCTCAGCCCACAGACACCTGAGCAGAAGGAATGGGCTTTCCAGACTCTGCCAGAGC
AGGACGGCGCTCTCTGAAGACAGATGGAGCTCCTATTGTCTATCATCACTGGCTGCCCAG
AATATTTGTACAAGTAAACTGCACTGCCCTGCTGCCCCTGAGCACACGGACCCGTCCGAA
CCGCGGGGCAGTGTGTCCTGCTGCTCCCTGCTGCGGGGACTGTCCTCAGGGTGGTCCTCA
CCTCTGCTTCCGGCCCCTGTGTGCAACCCTAACAAGGCCATCTTCACGGTGGATGCCAAG
ACCACAGAGATCCTGGTTGCTAACGACAAAGCTTGCGGGCTCCTGGGGTACAGCAGCCAG
GACCTGATTGGCCAGAAGCTCACGCAGTTCTTTCTGAGGTCAGATTCTGATGTGGTGGAG
GCCCTCAGCGAGGAGCACATGGAGGCCGACGGCCACGCTGCGGTGGTGTTTGGCACGGTG
GTGGACATCATCAGCCGTAGTGGGGAGAAGATTCCAGTGTCTGTGTGGATGAAGAGGATG
CGGCAGGAGCGCCGCCTATGCTGCGTGGTGGTCCTGGAGCCCGTGGAGAGGGTCTCGACC
TGGGTCGCTTTCCAGAGCGATGGCACCGTCACGTCATGTGACAGTCTCTTTGCTCATCTT
CACGGGTACGTGTCTGGGGAGGACGTGGCTGGGCAGCATATCACAGACCTGATCCCTTCT
GTGCAGCTCCCTCCTTCTGGCCAGCACATCCCAAAGAATCTCAAGATTCAGAGGTCTGTT
GGAAGAGCCAGGGACGGTACCACCTTCCCTCTGAGCTTAAAGCTGAAATCCCAACCCAGC
AGCGAGGAGGCGACCACCGGTGAGGCGGCCCCTGTGAGCGGCTACCGGGCATCTGTCTGG
GTGTTCTGCACCATCAGTGGCCTCATCACCCTCCTGCCGGATGGGACCATCCACGGCATC
AACCACAGCTTCGCGCTGACACTGTTTGGTTACGGAAAGACGGAGCTCCTGGGCAAGAAT
ATCACTTTCCTGATTCCTGGTTTCTACAGCTACATGGACCTTGCGTACAACAGCTCATTA
CAGCTCCCAGACCTGGCCAGCTGCCTGGACGTCGGCAATGAGAGTGGGTGTGGGGAGAGA
ACCTTGGACCCGTGGCAGGGCCAGGACCCAGCTGAGGGGGGCCAGGATCCAAGGATTAAT
GTCGTGCTTGCTGGTGGCCACGTTGTGCCCCGAGATGAGATCCGGAAGCTGATGGAAAGC
CAAGACATCTTCACCGGGACTCAGACTGAGCTGATTGCTGGAGGCCAGCTCCTTTCCTGC
CTCTCACCTCAGCCTGCTCCAGGGGTGGACAATGTCCCAGAAGGAAGCCTGCCAGTGCAC
GGTGAACAGGCGCTGCCCAAGGACCAGCAAATCACTGCCTTGGGGAGAGAGGAACCTGTG
GCAATAGAGAGCCCCGGACAGGATCTTCTGGGAGAAAGCAGGTCTGAACCAGTGGATGTG
AAGCCATTTGCTTCCTGCGAAGATTCTGAAGCTCCAGTCCCAGCTGAGGATGGGGGCAGT
GATGCTGGCATGTGTGGCCTGTGTCAGAAGGCCCAGCTAGAGCGGATGGGAGTCAGTGGT
CCCAGCGGTTCAGACCTTTGGGCTGGGGCTGCCGTGGCCAAGCCCCAGGCCAAGGGTCAG
CTGGCGGGGGGCAGCCTCCTGATGCACTGCCCTTGCTATGGGAGTGAATGGGGCTTGTGG
TGGCGAAGCCAGGACTTGGCCCCCAGCCCCTCTGGGATGGCAGGCCTCTCGTTTGGGACA
CCTACTCTAGATGAGCCGTGGCTGGGAGTGGAAAACGACCGAGAAGAGCTGCAGACCTGC
TTGATTAAGGAGCAGCTGTCCCAGTTGAGCCTTGCAGGAGCCCTGGATGTCCCCCACGCC
GAACTCGTTCCGACAGAGTGCCAGGCTGTCACCGCTCCTGTGTCGTCCTGCGATCTGGGA
GGCAGAGACCTGTGCGGTGGCTGCACGGGCAGCTCCTCAGCCTGCTATGCCTTGGCCACG
GACCTCCCTGGGGGCCTGGAAGCAGTGGAGGCCCAGGAGGTTGATGTGAATTCGTTTTCC
TGGAACCTCAAGGAACTCTTTTTCAGTGACCAGACAGACCAAACGTCATCAAATTGTTCC
TGTGCTACGTCTGAACTCAGAGAGACACCCTCTTCCTTGGCAGTGGGCTCCGATCCAGAT
GTAGGCAGTCTCCAGGAACAGGGGTCGTGTGTCCTGGATGACAGGGAGCTGTTACTACTG
ACCGGCACCTGTGTTGACCTTGGCCAAGGCCGACGGTTCCGGGAGAGCTGTGTGGGACAT
GATCCAACAGAACCGCTTGAGGTTTGTTTGGTGTCCTCTGAGCATTATGCAGCAAGCGAC
AGAGAAAGCCCAGGACACGTTCCTTCCACGTTGGATGCTGGCCCTGAGGACACGTGCCCA
TCAGCAGAGGAGCCAAGGCTGAACGTCCAGGTCACCTCCACGCCCGTGATCGTGATGCGC
GGGGCTGCTGGCCTGCAGCGGGAGATCCAGGAGGGTGCCTACTCCGGGAGCTGCTACCAT
CGAGATGGCTTACGGCTGAGTATACAGTTTGAGGTGAGGCGGGTGGAGCTCCAGGGCCCC
ACACCTCTGTTCTGCTGCTGGCTGGTGAAAGACCTCCTCCACAGCCAACGCGACTCAGCC
GCCAGGACCCGCCTGTTCCTTGCCAGCCTGCCCGGCTCCACCCACTCTACCGCTGCTGAG
CTCACCGGACCCAGCCTGGTGGAAGTGCTCAGAGCCAGACCCTGGTTTGAGGAGCCCCCC
AAGGCTGTGGAACTGGAGGGGTTGGCGGCCTGTGAGGGCGAGTACTCCCAAAAGTACAGT
ACCATGAGCCCGCTGGGCAGTGGGGCCTTCGGCTTCGTGTGGACTGCTGTGGACAAGGAA
AAAAACAAGGAGGTGGTGGTGAAGTTTATTAAGAAGGAGAAGGTCTTGGAGGATTGTTGG
ATTGAGGATCCCAAACTTGGGAAAGTTACTTTAGAGATCGCAATTCTATCCAGGGTGGAG
CACGCCAATATCATCAAGGTATTGGATATATTTGAAAACCAAGGGTTCTTCCAGCTTGTG
ATGGAGAAGCACGGCTCCGGCCTAGACCTCTTCGCTTTCATCGACCGCCACCCCAGGCTG
GATGAGCCCCTGGCGAGCTACATCTTCCGACAACTAGTGTCAGCAGTGGGATACCTGCGC
TTGAAGGACATCATCCACCGTGACATCAAGGATGAGAACATCGTGATCGCCGAGGACTTC
ACAATCAAGCTGATAGACTTTGGCTCGGCCGCCTACTTGGAAAGGGGAAAATTATTTTAT
ACTTTTTGTGGGACCATCGAGTACTGTGCACCGGAAGTTCTCATGGGGAATCCCTACAGA
GGGCCGGAGCTGGAGATGTGGTCTCTGGGAGTCACTCTGTACACGCTGGTCTTTGAGGAG
AACCCCTTCTGTGAGCTGGAGGAGACCGTGGAGGCTGCCATACACCCGCCATACCTGGTG
TCCAAAGAACTCATGAGCCTTGTGTCTGGGCTGCTGCAGCCAGTCCCTGAGAGACGCACC
ACCTTGGAGAAGCTGGTGACAGACCCGTGGGTAACACAGCCTGTGAATCTTGCTGACTAT
ACATGGGAAGAGGTGTTTCGAGTAAACAAGCCAGAAAGTGGAGTTCTGTCCGCTGCGAGC
CTGGAGATGGGGAACAGGAGCCTGAGTGATGTGGCCCAGGCTCAGGAGCTTTGTGGGGGC
CCCGTTCCAGGCGAGGCTCCTAATGGCCAAGGCTGTTTGCATCCCGGGGATCCCCGTCTG
CTGACCAGCTTG

Protein sequence predicted based on nucleotide sequence

MEDGGLTAFEEDQRCLSQSLPLPVSAEGPAAQTTAEPSRSFSSAHRHLSRRNGLSRLCQS
RTALSEDRWSSYCLSSLAAQNICTSKLHCPAAPEHTDPSEPRGSVSCCSLLRGLSSGWSS
PLLPAPVCNPNKAIFTVDAKTTEILVANDKACGLLGYSSQDLIGQKLTQFFLRSDSDVVE
ALSEEHMEADGHAAVVFGTVVDIISRSGEKIPVSVWMKRMRQERRLCCVVVLEPVERVST
WVAFQSDGTVTSCDSLFAHLHGYVSGEDVAGQHITDLIPSVQLPPSGQHIPKNLKIQRSV
GRARDGTTFPLSLKLKSQPSSEEATTGEAAPVSGYRASVWVFCTISGLITLLPDGTIHGI
NHSFALTLFGYGKTELLGKNITFLIPGFYSYMDLAYNSSLQLPDLASCLDVGNESGCGER
TLDPWQGQDPAEGGQDPRINVVLAGGHVVPRDEIRKLMESQDIFTGTQTELIAGGQLLSC
LSPQPAPGVDNVPEGSLPVHGEQALPKDQQITALGREEPVAIESPGQDLLGESRSEPVDV
KPFASCEDSEAPVPAEDGGSDAGMCGLCQKAQLERMGVSGPSGSDLWAGAAVAKPQAKGQ
LAGGSLLMHCPCYGSEWGLWWRSQDLAPSPSGMAGLSFGTPTLDEPWLGVENDREELQTC
LIKEQLSQLSLAGALDVPHAELVPTECQAVTAPVSSCDLGGRDLCGGCTGSSSACYALAT
DLPGGLEAVEAQEVDVNSFSWNLKELFFSDQTDQTSSNCSCATSELRETPSSLAVGSDPD
VGSLQEQGSCVLDDRELLLLTGTCVDLGQGRRFRESCVGHDPTEPLEVCLVSSEHYAASD
RESPGHVPSTLDAGPEDTCPSAEEPRLNVQVTSTPVIVMRGAAGLQREIQEGAYSGSCYH
RDGLRLSIQFEVRRVELQGPTPLFCCWLVKDLLHSQRDSAARTRLFLASLPGSTHSTAAE
LTGPSLVEVLRARPWFEEPPKAVELEGLAACEGEYSQKYSTMSPLGSGAFGFVWTAVDKE
KNKEVVVKFIKKEKVLEDCWIEDPKLGKVTLEIAILSRVEHANIIKVLDIFENQGFFQLV
MEKHGSGLDLFAFIDRHPRLDEPLASYIFRQLVSAVGYLRLKDIIHRDIKDENIVIAEDF
TIKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLMGNPYRGPELEMWSLGVTLYTLVFEE
NPFCELEETVEAAIHPPYLVSKELMSLVSGLLQPVPERRTTLEKLVTDPWVTQPVNLADY
TWEEVFRVNKPESGVLSAASLEMGNRSLSDVAQAQELCGGPVPGEAPNGQGCLHPGDPRL
LTSL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 4064
Start on reference sequence 93
Mutations:
None
Discrepancy:
a186g
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable