Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
C-His_Rv2021c
Keyword:
None
Species:
Mycobacterium tuberculosis H37Rv
Type:
cDNA
Vector Name:
pET28b-C-His
Format: 
FUSION
Source:
Integrated Center for Structure and Function Innovation
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Integrated Center for Structure and Function Innovation
MtCD00385973
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 303nts
Open reading frame: 36 to 338

AAATATTTTGTTTACTTTAAGAAGGAGATATACATATGGCGATGACACTACGGGACATGG
ACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCGCGATG
AAGTGCGGGCATTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGG
TGGCTGCGCTGGCGCACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCG
GCTCCGCACAGGTCAACACACTGCGCAAATACGTGTCCGCTCTTGGCGGAGAACTGGATA
TCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCAGGATCCCACCACCACCACCACC
ACTAACTCGAGCACCACCACCACCACCAC

Protein sequence predicted based on nucleotide sequence

MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRV
SSIENGDIGSAQVNTLRKYVSALGGELDITVRLGDETFTLA

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in Rv2021c
Recommended expression in:
Not Applicable