WS2113 (W. succinogenes DSM 1740 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  WS2113
Gene Name:  hypothetical protein
Original Clone ID:
11227j3BCt1p1
Keyword:
None
Species:
Wolinella succinogenes DSM 1740
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified. Actual 5' flanking sequence provided below.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
WsCD00386064
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1236nts
Open reading frame: 51 to 1286

TTCCTCTGAATATTTTGTTAACTTTAGAAGGAGATATACATATGTCCCTTGATAGTCGCA
CCGAGCTCAAAGAGATTGCTGGGGTCGCCAAGCGCCTTACGCTCCTTTATGTCGAGGATG
ACATTCCCACAAGACGCTTTTTAAAGCGGCGCTTGGAGGAGATATTTGGGGTGGTCGCAG
AGGCCGCCAATGGCGAGGAAGGATTGAAGCGATTCCTTCAAGGAGGGATTGATCTCGTTA
TCTCAGATAATATCATGCCCTTTATGGATGGAATCGAGATGATTCGAGAGATTCGCCGTG
TCGATACTAGGCTCCCCATCATCCTCACCACCGCCTTTATTGATACCGACTATCTGATGG
AGGCGATCAATTTAGGGGTGACCCAATTTGTGGCCAAGCCCATCTCCATGGATCTTCTTC
ATAATGCCATCCAAATGGCCATCCAGCGGGTGGTGCTGGATAACCTGACCCGCAAAGCCA
AAGAGCAGGAGATTGAACTGCTGCGCTTTCAAGATCGCTACCACTCCCTCCAGCAAGAAT
CAGCCAGACGCAAAGAGCGAAACCTCATCAAAAATGATCACTACCTCCAGACCATCCAAG
GAAAAGAGGGAGAGGAGTGGGTGCTGGACGCGCTTCACCGTTCCTTGGAGATTTTGAGTG
GGGATAGCTATTCGGTGCGAGGCCTAGATGAGGGGAGGGCGCTGCTTTTTATTGTGGATG
GAATGGGCAAGGGACTCTCCGCCTCGGTCACCACCACCCTCTCTATCGCTTTTTTGAATT
ACCTCATCGATGAGAGAATCGCTCAAGGGGCGAGGGTGGAGCTTGGCTCTTTGGTGGGGG
TCTACTTACGCTTCATTCAAAAAGAGCTTCTTGAAGAGGAGATCATCAGCGCGGGATTTT
ACGACTTTGATTTCAAGAGAGAGCGCCTCTCTTACATTACTTTTGGAATGCCCTCCATGC
TATGGGTTAATAGCCAAGGGGAGTGCGAGCGTATCAAATCCTCTTCGCCCCCGCTCATGA
AATACTCCCTTCCGCCTTTGCCACAGGAGATATGCATCCAAGATTTGGAGAAGATTCTAC
TTTATAGCGATGGGCTTAATGAGGCTTTGCTGGATGAAGAGACGATCTATGGAGAGATTT
TGGAGGAGGAGTTGCTTTGGAATCGATTCCTCTCGGAGATGAATCGACGCTTTAGGAGTC
GAGTGAAAGATGTGGATGATGACATCACCGCCTTTTGGGTGCGAAGGGTCCCCCAAAATG
AAGGGCATCATCACCATCACCACTGATCGGAATTCGAGCTC

Protein sequence predicted based on nucleotide sequence

DSRTELKEIAGVAKRLTLLYVEDDIPTRRFLKRRLEEIFGVVAEAANGEEGLKRFLQGGI
DLVISDNIMPFMDGIEMIREIRRVDTRLPIILTTAFIDTDYLMEAINLGVTQFVAKPISM
DLLHNAIQMAIQRVVLDNLTRKAKEQEIELLRFQDRYHSLQQESARRKERNLIKNDHYLQ
TIQGKEGEEWVLDALHRSLEILSGDSYSVRGLDEGRALLFIVDGMGKGLSASVTTTLSIA
FLNYLIDERIAQGARVELGSLVGVYLRFIQKELLEEEIISAGFYDFDFKRERLSYITFGM
PSMLWVNSQGECERIKSSSPPLMKYSLPPLPQEICIQDLEKILLYSDGLNEALLDEETIY
GEILEEELLWNRFLSEMNRRFRSRVKDVDDDITAFWVRRVPQNEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a791t,D264V
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-11227j
Recommended expression in:
Not Applicable