YPTB2534 (Y. pseudotuberculosis IP 32953 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  YPTB2534
Gene Name:  LacI family transcriptional regulatory protein
Original Clone ID:
11235b1BCt2p1
Keyword:
None
Species:
Yersinia pseudotuberculosis IP 32953
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified. Actual 5' flanking sequence provided below. High number of silent and/or missense mutations in CDS. Reference sequence corrected.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
YpCD00386066
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1011nts
Open reading frame: 51 to 1061

TTCCTCTGAATATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTAATGCCACGA
TTGTGGATATTGCCCGTCGAGCTAATGTGACAAACATCACGGTTTCCCGTACTTTTAATC
AACCTGGATTAGTTAAACAAGAAACCCGTGAAAAAATTCATGCTATCGCAAAAGAACTTA
ATTACGTCCCCAATGCCTTTGCTCAGGGATTAAAACGCAGTAGCAGCCAAATTATTGGTA
TTGTCACCAGCAGTATGTATAACCCTTTTTACTCTGAATTAATTCAAACGGTCTCCAGAA
TTGCACGAACACAGTGTTATCAAATTATGCTGTTTGATACCGATGGCAGTGAAGAGGCAG
AAATGGAGGCTATTCAGGCGCTATTTGGCTATAAAGCCTGTGGAATTCTTTTGTCACCGG
TTCGCGATGATAAAAACTACCAACCGGCCTATCTCGATTTAGCCGAAACCTATCGCGTTC
CATTGATTCTTATTGATAGAGATATCTATAATCGGCAACTGAGCGGAGTATTTCTTAATA
ACAAAGAGATCGGGTTACTGGCGGGGAAATACCTGTCTGAGCAGCCAGAAAGGAAAATGC
TGATCATTGGTGGGCCCGCAGAATCAGAGATAACACGGGTACGTATTGAAGGGATTATCA
ATGCGTTAGAAAATAAAAAAAATGACGTACATATTATTAATGGTGATTACGATTTTATTT
CACAGGAAGCTACCGTTAGAGATTATCTCTCTGCCCCTCAGAATCAACCTGATTATATTA
TTGGTTTAAATGGAATTTTGACGCTTGGCGCTATTGCAATTTGTCATGAACTAAAAATTT
ATAATAATATAAAATTCTTCTCGATTGATGAGCCACCTAAAGCAGCAGATTATGGCCTTC
ATATTGCCGGTGTTTATCACGATACCCAGATGCTGGGGGAGATTGCTGCAGAACTCCTTT
TTAATGCCATTAAAAAACCCTACAACGATCAGGCAGTAAGACGGGAATTCTTCACCGGAT
CTCTGCTCACCCAGGAAGGGCATCATCACCATCACCACTGATCGGAAT

Protein sequence predicted based on nucleotide sequence

NATIVDIARRANVTNITVSRTFNQPGLVKQETREKIHAIAKELNYVPNAFAQGLKRSSSQ
IIGIVTSSMYNPFYSELIQTVSRIARTQCYQIMLFDTDGSEEAEMEAIQALFGYKACGIL
LSPVRDDKNYQPAYLDLAETYRVPLILIDRDIYNRQLSGVFLNNKEIGLLAGKYLSEQPE
RKMLIIGGPAESEITRVRIEGIINALENKKNDVHIINGDYDFISQEATVRDYLSAPQNQP
DYIIGLNGILTLGAIAICHELKIYNNIKFFSIDEPPKAADYGLHIAGVYHDTQMLGEIAA
ELLFNAIKKPYNDQAVRREFFTGSLLTQEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11235b
Recommended expression in:
Not Applicable