BRD3 (Homo sapiens) in pJP1520 (retroviral expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BRD3
Gene Name:  bromodomain containing 3
Original Clone ID:
FLH205534.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJP1520
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HsCD00038612
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2181nts
Open reading frame: 1 to 2181

ATGTCCACCGCCACGACAGTCGCCCCCGCGGGGATCCCGGCGACCCCGGGCCCTGTGAAC
CCACCCCCCCCGGAGGTCTCCAACCCCAGCAAGCCCGGCCGCAAGACCAACCAGCTGCAG
TACATGCAGAATGTGGTGGTGAAGACGCTCTGGAAACACCAGTTCGCCTGGCCCTTCTAC
CAGCCCGTGGACGCAATCAAATTGAACCTGCCGGATTATCATAAAATAATTAAAAACCCA
ATGGATATGGGGACTATTAAGAAGAGACTAGAAAATAATTATTATTGGAGTGCAAGCGAA
TGTATGCAGGACTTCAACACCATGTTTACAAATTGTTACATTTATAACAAGCCCACAGAT
GACATAGTGCTAATGGCCCAAGCTTTAGAGAAAATTTTTCTACAAAAAGTGGCCCAGATG
CCCCAAGAGGAAGTTGAATTATTACCCCCTGCTCCAAAGGGCAAAGGTCGGAAGCCGGCT
GCGGGAGCCCAGAGCGCAGGTACACAGCAAGTGGCGGCCGTGTCCTCTGTCTCCCCAGCG
ACCCCCTTTCAGAGCGTGCCCCCCACCGTCTCCCAGACGCCCGTCATCGCTGCCACCCCT
GTACCAACCATCACTGCAAACGTCACGTCGGTCCCAGTCCCCCCAGCTGCCGCCCCACCT
CCTCCTGCCACACCCATCGTCCCCGTGGTCCCTCCTACGCCGCCTGTCGTCAAGAAAAAG
GGCGTGAAGCGGAAAGCAGACACAACCACTCCCACGACGTCGGCCATCACTGCCAGCCGG
AGTGAGTCGCCCCCGCCGTTGTCAGACCCCAAGCAGGCCAAAGTGGTGGCCCGGCGGGAG
AGTGGTGGCCGCCCCATCAAGCCTCCCAAGAAGGACCTGGAGGACGGCGAGGTGCCCCAG
CACGCAGGCAAGAAGGGCAAGCTGTCGGAGCACCTGCGCTACTGCGACAGCATCCTCAGG
GAGATGCTATCCAAGAAGCACGCGGCCTACGCCTGGCCCTTCTACAAGCCAGTGGATGCC
GAGGCCCTGGAGCTGCACGACTACCACGACATCATCAAGCACCCGATGGACCTCAGCACC
GTGAAAAGGAAGATGGATGGCCGAGAGTACCCAGACGCACAGGGCTTTGCTGCTGATGTC
CGGCTGATGTTCTCGAATTGCTACAAATACAATCCCCCAGACCACGAGGTTGTGGCCATG
GCCCGGAAGCTCCAGGACGTGTTTGAGATGAGGTTTGCCAAGATGCCAGATGAGCCCGTG
GAGGCACCGGCGCTGCCTGCCCCCGCGGCCCCCATGGTGAGCAAGGGCGCTGAGAGCAGC
CGTAGCAGTGAGGAGAGCTCTTCGGACTCAGGCAGCTCGGACTCGGAGGAGGAGCGGGCC
ACCAGGCTGGCGGAGCTGCAGGAGCAGCTGAAGGCCGTGCACGAGCAGCTGGCCGCCCTG
TCTCAGGCCCCAGTAAACAAACCAAAGAAGAAGAAGGAGAAGAAGGAGAAGGAGAAGAAG
AAGAAGGACAAGGAGAAGGAGAAGGAGAAGCACAAAGTGAAGGCCGAGGAAGAGAAGAAG
GCCAAGGTGGCTCCGCCTGCCAAGCAGGCTCAGCAGAAGAAGGCTCCTGCCAAGAAGGCC
AACAGCACGACCACGGCCGGCAGACAGCTGAAGAAAGGCGGCAAGCAGGCATCTGCCTCC
TACGACTCAGAGGAAGAGGAGGAGGGCCTGCCCATGAGCTACGATGAAAAGCGCCAGCTT
AGCCTGGACATCAACCGGCTGCCCGGGGAGAAGCTGGGCCGGGTAGTGCACATCATCCAA
TCTCGGGAGCCCTCGCTCAGGGACTCCAACCCCGACGAGATAGAAATTGACTTTGAGACT
CTGAAACCCACCACTTTGCGGGAACTGGAGAGATATGTCAAGTCTTGTTTACAGAAAAAG
CAAAGGAAACCGTTCTCAGCAAGCGGGAAGAAACAGGCAGCCAAGTCGAAAGAGGAGCTA
GCTCAGGAAAAGAAGAAGGAGCTGGAAAAGCGTCTGCAGGATGTCAGCGGGTAGCTGAGC
AGCAGCAAGAAGCCCGCCCGGAAAGAGAAGCCCGGCTCAGCACCCTCAGGGGGCCCGTCC
AGGCTCAGCAGCAGCAGCTCCTCCGAGTCTGGGAGCAGCAGCTCCAGCGGGTCCAGCTCT
GACAGCAGTGACTCAGAATTG

Protein sequence predicted based on nucleotide sequence

MSTATTVAPAGIPATPGPVNPPPPEVSNPSKPGRKTNQLQYMQNVVVKTLWKHQFAWPFY
QPVDAIKLNLPDYHKIIKNPMDMGTIKKRLENNYYWSASECMQDFNTMFTNCYIYNKPTD
DIVLMAQALEKIFLQKVAQMPQEEVELLPPAPKGKGRKPAAGAQSAGTQQVAAVSSVSPA
TPFQSVPPTVSQTPVIAATPVPTITANVTSVPVPPAAAPPPPATPIVPVVPPTPPVVKKK
GVKRKADTTTPTTSAITASRSESPPPLSDPKQAKVVARRESGGRPIKPPKKDLEDGEVPQ
HAGKKGKLSEHLRYCDSILREMLSKKHAAYAWPFYKPVDAEALELHDYHDIIKHPMDLST
VKRKMDGREYPDAQGFAADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPV
EAPALPAPAAPMVSKGAESSRSSEESSSDSGSSDSEEERATRLAELQEQLKAVHEQLAAL
SQAPVNKPKKKKEKKEKEKKKKDKEKEKEKHKVKAEEEKKAKVAPPAKQAQQKKAPAKKA
NSTTTAGRQLKKGGKQASASYDSEEEEEGLPMSYDEKRQLSLDINRLPGEKLGRVVHIIQ
SREPSLRDSNPDEIEIDFETLKPTTLRELERYVKSCLQKKQRKPFSASGKKQAAKSKEEL
AQEKKKELEKRLQDVSG*LSSSKKPARKEKPGSAPSGGPSRLSSSSSSESGSSSSSGSSS
DSSDSEL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
Start on reference sequence 140
End on reference sequence 2320
Mutations:
None
Discrepancy:
c2032t,Q678*;
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable