MAGI1 (Homo sapiens) in pJP1520 (retroviral expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MAGI1
Gene Name:  membrane associated guanylate kinase, WW and PDZ domain containing 1
Original Clone ID:
FLH205932.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJP1520
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HsCD00038667
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3651nts
Open reading frame: 1 to 3651

ATGTCCAAAGTGATCCAGAAGAAGAACCACTGGACTAGCAGGGTTCACGAATGCACCGTG
AAGCGGGGACCCCAGGGCGAGCTGGGGGTGACGGTGCTGGGAGGCGCGGAGCACGGGGAG
TTTCCGTACGTCGGAGCGGTGGCGGCGGTCGAGGCAGCGGGGCTTCCCGGCGGCGGCGAG
GGCCCGAGGCTGGGCGAAGGGGAGCTGCTTCTGGAGGTGCAGGGGGTCCGGGTGTCCGGC
TTGCCCCGCTATGACGTGCTGGGGGTCATCGACAGCTGCAAGGAGGCCGTCACCTTCAAG
GCCGTCAGACAAGGAGGAAGGCTGAACAAGGACCTGCGACATTTTCTCAATCAGCGATTC
CAGAAGGGGTCTCCTGATCATGAGCTCCAGCAGACCATAAGGGATAACCTTTACCGCCAT
GCTGTGCCTTGCACAACCCGATCTCCCAGAGAAGGAGAAGTGCCTGGCGTGGACTATAAC
TTTCTGACTGTGAAGGAGTTCTTGGACCTCGAGCAGAGTGGGACTCTTCTGGAAGTCGGC
ACCTATGAAGGAAACTATTATGGGACACCCAAGCCTCCTAGCCAGCCAGTCAGTGGGAAA
GTGATCACGACGGATGCCTTGCACAGCCTTCAGTCTGGCTCTAAGCAGTCGACCCCGAAG
CGAACCAAGTCCTACAATGATATGCAAAATGCTGGCATAGTCCACGCGGAGAATGAGGAG
GAGGATGACGTTCCTGAAATGAACAGCAGCTTTACAGCCGATTCTGGTGAACAAGAGGAG
CACACTCTCCAAGAAACAGCATTACCACCTGTGAATAGTAGCATCATCGCTGCTCCCATC
ACGGACCCTTCTCAGAAGTTCCCTCAATACCTACCTCTTTCTGCAGAGGATAATTTAGGT
CCTCTACCTGAAAACTGGGAGATGGCCTATACTGAAAATGGAGAAGTCTATTTTATAGAC
CATAACACGAAAACAACATCTTGGTTAGACCCTCGGTGCCTAAACAAGCAGCAGAAGCCA
CTGGAAGAGTGTGAAGATGATGAAGGGGTACACACCGAGGAGCTGGACAGTGAACTAGAA
CTGCCTGCTGGTTGGGAAAAGATTGAAGACCCTGTCTATGGTATCTACTATGTAGACCAC
ATCAACAGGAAGACACAATATGAGAACCCGGTTCTAGAAGCCAAACGGAAGAAGCAGCTT
GAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAG
CAGACAGAAGAATGGACAGAAGATCACTCAGCCCTTGTGCCTCCTGTTATTCCAAACCAC
CCTCCAAGCAATCCAGAGCCAGCCAGAGAAGTTCCACTTCAGGGCAAACCCTTTTTTACA
CGAAACCCTTCTGAGTTGAAAGGCAAGTTCATTCACACAAAGCTGCGGAAAAGCAGTCGT
GGCTTTGGCTTCACGGTGGTTGGAGGGGATGAACCTGATGAGTTTCTCCAGATCAAGAGC
TTGGTCCTAGATGGTCCTGCTGCATTGGATGGCAAGATGGAAACAGGGGATGTGATTGTA
AGTGTGAATGACACCTGTGTTTTGGGACACACACATGCTCAAGTTGTGAAGATCTTCCAG
TCCATCCCCATTGGTGCCAGCGTGGACCTTGAACTCTGCCGAGGTTATCCATTGCCTTTT
GATCCAGATGACCCCAATACAAGTTTAGTGACCTCGGTAGCCATTTTGGATAAAGAACCA
ATTATTGTGAATGGGCAAGAGACCTATGATTCACCAGCTAGCCACAGTAGTAAAACAGGC
AAAGTCAATGGCATGAAGGATGCCAGGCCAAGCAGCCCAGCGGACGTGGCTTCAAATAGT
TCTCATGGTTATCCTAATGACACTGTTTCTTTGGCTTCCTCCATAGCCACTCAGCCAGAA
CTCATAACTGTTCATATTGTCAAAGGGCCAATGGGCTTTGGTTTTACTATCGCAGACAGT
CCTGGTGGGGGTGGCCAAAGAGTGAAACAGATTGTTGACAGCCCAAGGTGCCGAGGCCTG
AAAGAAGGGGATCTCATAGTGGAAGTTAATAAGAAGAACGTGCAGGCCCTAACTCACAAC
CAAGTGGTGGATATGCTGGTTGAGTGTCCTAAGGGAAGTGAGGTCACATTGTTGGTGCAA
CGAGGAGGGCTGCCAGTTCCCAAGAAGAGCCCAAAGTCGCAACCACTGGAAAGGAAAGAC
AGCCAGAATAGTTCTCAGCACAGTGTTTCCAGCCACCGAAGCCTGCACACAGCATCCCCA
AGCCACAGCACACAGGTGCTCCCCGAGTTCCCACCTGCAGAGGCCCAAGCTCCAGATCAA
ACTGACAGCTCTGGCCAGAAAAAACCAGATCCTTTTAAAATCTGGGCCCAGTCCAGGAGC
ATGTATGAAAACCGACTTCCAGATTACCAGGAACAGGACATCTTCCTCTGGAGAAAAGAG
ACTGGATTTGGATTTAGGATTCTGGGTGGAAATGAACCAGGGGAACCTATTTATATTGGT
CACATCGTACCACTGGGTGCTGCTGATACTGACGGCCGCCTGAGGTCTGGAGATGAATTA
ATCTGTGTGGATGGGACGCCAGTAATTGGAAAATCACACCAGCTTGTGGTCCAGCTTATG
CAACAAGCTGCCAAGCAAGGCCACGTCAATCTCACGGTGCGGCGTAAAGTGGTTTTTGCG
GTGCCCAAAACCGAGAACGAGGTGCCCTCGCCAGCCTCCTCTCATCACAGTAGCAACCAG
CCGGCCTCGCTGACAGAAGAGAAGCGCACTCCGCAGGGCAGCCAGAACTCGCTGAACACG
GTGAGCTCGGGCAGCGGCAGCACCAGCGGCATCGGCAGTGGCGGCGGCGGGGGCAGCGGC
GTGGTCAGCACCGTGGTGCAGCCCTACGACGTGGAGATCCGGCGCGGGGAGAACGAGGGC
TTCGGCTTCGTCATCGTGTCCTCGGTGAGCAGGCCCGAAGCAGGCACGACCTTTGGTCGG
ATTATTGAGGGGAGCCCTGCTGACCGCTGTGGCAAGCTGAAAGTAGGAGACCGGATCTTG
GCAGTAAATGGATGTTCCATCACCAACAAATCCCATTCAGACATTGTGAACCTAATCAAG
GAAGCGGGAAACACAGTTACCCTCCGCATCATTCCTGGGGATGAGTCCTCGAATGCCACC
TTGCTGACCAATGCAGAGAAGATTGCCACCATCACCACCACACACACCCCTTCTCAGCAA
GGGACCCAGGAGACAAGGAATACCACCAAACCAAAGCAGGAATCTCAATTTGAGTTCAAA
GCACCCCAAGCAACACAGGAGCAAGATTTTTACACTGTGGAACTGGAAAGAGGAGCCAAG
GGATTTGGCTTTAGCCTTCGAGGAGGCCGAGAGTATAACATGGACCTCTATGTTCTGCGC
TTAGCAGAGGACGGTCCTGCGGAGAGGTGTGGAAAGATGAGGATTGGTGATGAAATTTTA
GAGATCAATGGTGAGACCACCAAAAACATGAAGCATTCTCGAGCTATAGAACTGATTAAG
AATGGTGGCCGCAGAGTTCGTCTGTTTCTGAAGCGGGGAGACGGCTCAGTACCAGAATAT
GGTGGGTCAAACTATGAAAACATTCCTTCCTTCCCTGGCATGACTCCATTG

Protein sequence predicted based on nucleotide sequence

MSKVIQKKNHWTSRVHECTVKRGPQGELGVTVLGGAEHGEFPYVGAVAAVEAAGLPGGGE
GPRLGEGELLLEVQGVRVSGLPRYDVLGVIDSCKEAVTFKAVRQGGRLNKDLRHFLNQRF
QKGSPDHELQQTIRDNLYRHAVPCTTRSPREGEVPGVDYNFLTVKEFLDLEQSGTLLEVG
TYEGNYYGTPKPPSQPVSGKVITTDALHSLQSGSKQSTPKRTKSYNDMQNAGIVHAENEE
EDDVPEMNSSFTADSGEQEEHTLQETALPPVNSSIIAAPITDPSQKFPQYLPLSAEDNLG
PLPENWEMAYTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEGVHTEELDSELE
LPAGWEKIEDPVYGIYYVDHINRKTQYENPVLEAKRKKQLEQQQQQQQQQQQQQQQQQQQ
QTEEWTEDHSALVPPVIPNHPPSNPEPAREVPLQGKPFFTRNPSELKGKFIHTKLRKSSR
GFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGHTHAQVVKIFQ
SIPIGASVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETYDSPASHSSKTG
KVNGMKDARPSSPADVASNSSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADS
PGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQALTHNQVVDMLVECPKGSEVTLLVQ
RGGLPVPKKSPKSQPLERKDSQNSSQHSVSSHRSLHTASPSHSTQVLPEFPPAEAQAPDQ
TDSSGQKKPDPFKIWAQSRSMYENRLPDYQEQDIFLWRKETGFGFRILGGNEPGEPIYIG
HIVPLGAADTDGRLRSGDELICVDGTPVIGKSHQLVVQLMQQAAKQGHVNLTVRRKVVFA
VPKTENEVPSPASSHHSSNQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSG
VVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFGRIIEGSPADRCGKLKVGDRIL
AVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNATLLTNAEKIATITTTHTPSQQ
GTQETRNTTKPKQESQFEFKAPQATQEQDFYTVELERGAKGFGFSLRGGREYNMDLYVLR
LAEDGPAERCGKMRIGDEILEINGETTKNMKHSRAIELIKNGGRRVRLFLKRGDGSVPEY
GGSNYENIPSFPGMTPL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
Start on reference sequence 243
End on reference sequence 4013
Mutations:
None
Discrepancy:
t371c,F124S;del@2417,84;
del@3079,36;
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable