HIPK3 (Homo sapiens) in pJP1563 (retroviral expression vector)

Explanation of Terms

Gene:
Gene Symbol:  HIPK3
Gene Name:  homeodomain interacting protein kinase 3
Original Clone ID:
FLH214188.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJP1563
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HsCD00038848
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3585nts
Open reading frame: 1 to 3585

ATGGCCTCACAAGTCTTGGTCTACCCACCATATGTTTATCAAACTCAGTCAAGTGCCTTT
TGTAGTGTGAAGAAACTCAAAGTAGAGCCAAGCAGTTGTGTATTCCAGGAAAGAAACTAT
CCACGGACCTATGTGAATGGTAGAAACTTTGGAAATTCTCATCCTCCCACTAAGGGTAGT
GCTTTTCAGACAAAGATACCATTTAATAGACCTCGAGGACACAACTTTTCATTGCAGACA
AGTGCTGTTGTTTTGAAAAACACTGCAGGTGCTACAAAGGTCATAGCAGCTCAGGCACAG
CAAGCTCACGTGCAGGCACCTCAGATTGGGGCGTGGCGAAACAGATTGCATTTCCTAGAA
GGCCCCCAGCGATGTGGATTGAAGCGCAAGAGTGAGGAGTTGGATAATCATAGCAGCGCA
ATGCAGATTGTCGATGAATTGTCCATACTTCCTGCAATGTTGCAAACCAACATGGGAAAT
CCAGTGACAGTTGTGACAGCTACCACAGGATCAAAACAGAATTGTACCACTGGAGAAGGT
GACTATCAGTTAGTACAGCATGAAGTCTTATGCTCCATGAAAAATACTTACGAAGTCCTT
GATTTTCTTGGTCGAGGCACGTTTGGCCAGGTAGTTAAATGCTGGAAAAGAGGGACAAAT
GAAATTGTAGCAATCAAAATTTTGAAGAATCATCCTTCTTATGCCCGTCAAGGTCAAATA
GAAGTGAGCATATTAGCAAGGCTCAGTACTGAAAATGCTGATGAATATAACTTTGTACGA
GCTTATGAATGCTTTCAGCACCGTAACCATACTTGTTTAGTCTTTGAGATGCTGGAACAA
AACTTGTATGACTTTCTGAAACAAAATAAATTTAGTCCCCTGCCACTAAAAGTGATTCGG
CCCATTCTTCAACAAGTGGCCACTGCACTGAAAAAATTGAAAAGTCTTGGTTTAATTCAT
GCTGATCTCAAGCCAGAGAATATTATGTTGGTGGATCCTGTTCGGCAGCCTTACAGGGTT
AAAGTAATAGACTTTGGGTCGGCCAGTCATGTATCAAAGACTGTTTGTTCAACATATCTA
CAATCTCGGTACTACAGAGCTCCAGAGATTATATTGGGGTTGCCATTTTGTGAAGCCATA
GACATGTGGTCATTGGGATGTGTGATTGCAGAATTATTTCTTGGATGGCCGCTCTACCCA
GGAGCCTTGGAGTATGATCAGATTCGATACATTTCTCAGACTCAAGGTTTGCCAGGAGAA
CAGTTGTTAAATGTGGGTACTAAATCCACAAGATTTTTTTGCAAAGAAACAGATATGTCT
CATTCTGGTTGGAGATTAAAGACATTGGAAGAGCATGAGGCAGAGACAGGAATGAAGTCT
AAAGAAGCCAGAAAATACATTTTCAACAGTCTGGATGATGTAGCGCATGTGAACACAGTG
ATGGATTTGGAAGGAAGTGATCTTTTGGCTGAGAAAGCTGATAGAAGAGAATTTGTTAGT
CTGTTGAAGAAAATGTTGCTGATTGATGCAGATTTAAGAATTACTCCAGCTGAGACCCTG
AACCATCCTTTTGTTAATATGAAACATCTTCTAGATTTCCCTCATAGCAACCATGTAAAG
TCCTGTTTTCATATTATGGATATTTGTAAGTCCCACCTAAATTCATGTGACACAAATAAT
CACAACAAAACTTCACTTTTAAGACCAGTTGCTTCAAGCAGTACTGCTACACTGACTGCA
AATTTTACTAAAATCGGAACATTAAGAAGTCAGGCATTGACCACATCTGCTCATTCAGTT
GTGCACCATGGAATACCTCTGCAGGCAGGAACTGCTCAGTTTGGTTGTGGTGATGCTTTT
CAGCAGACATTGATTATCTGTCCCCCAGCTATTCAAGGTATTCCTGCAACACATGGTAAA
CCCACCAGTTATTCAATAAGGGTAGATAATACAGTTCCACTTGTAACTCAGGCCCCAGCT
GTGCAGCCACTACAGATCCGACCAGGAGTTCTTTCTCAGACGTGGTCTGGTAGAACACAG
CAGATGCTGGTGCCTGCCTGGCAACAGGTGACACCCCTGGCTCCTGCTACTACTACACTA
ACTTCTGAGAGTGTGGCTGGTTCACACAGGCTTGGAGACTGGGGGAAGATGATTTCATGC
AGCAATCATTATAACTCAGTGATGCCGCAGCCTCTTCTGACCAATCAGATAACTTTATCT
GCCCCTCAGCCAGTTAGTGTGGGGATTGCACATGTTGTCTGGCCTCAGCCTGCCACTACC
AAGAAAAATAAACAGTGCCAGAACAGGAGTAATTCATTACAGAATACCAATATCCCACAT
TCAGCATTTATTTCTCCAAAGATAATTAATGGGAAAGATGTCGAGGAAGTAAGTTGTATA
GAAACACAGGACAATCAGAACTCAGAAGGAGAGGCAAGAAATTGCTGTGAAACATCTATC
AGACAGGACTCTGATTCATCAGTTTCAGACAAACAGCGGCAAACCATCATTATTGCCGAC
TCCCCGAGTCCTGCAGTGAGTGTCATCACTATCAGCAGTGACACTGATGAGGAAGAGACT
TCCCAGAGACATTCACTCAGAGAATGTAAAGGTAGTCTAGATTGTGAAGCTTGCCAGAGC
ACTTTGAATATTGATCGGATGTGTTCATTAAGTAGTCCTGATAGTACTCTGAGTACCAGC
TCCTCAGGGCAGTCCAGCCCATCCCCCTGCAAGAGACCGAATAGTATGTCAGATGAAGAG
CAAGAAAGTAGTTGTGATACGGTGGATGGCTCTCCGACATCTGACTCTTCCGGGCATGAC
AGTCCATTTGCAGAGAGCACTTTTGTGGAGGACACTCATGAAAACACAGAATTGGTATCC
TCTGCTGACACAGAAACCAAGCCAGCTGTCTGTTCTGTTGTGGTGCCACCAGTGGAACTA
GAAAATGGCTTAAATGCCGATGAGCATATGGCAAACACAGATTCTATATGCCAGCCATTA
ATAAAAGGACGATCTGCCCCTGGAAGATTAAACCAGCCTTCTGCAGTGGGTACTCGTCAG
CAAAAATTGACATCAGCATTCCAGCAGCAGCATTTGAACTTCAGTCAGGTTCAGCACTTT
GGATCTGGGCATCAAGAGTGGAATGGAAACTTTGGGCACAGAAGACAGCAAGCTTATATT
CCTACTAGTGTTACCAGTAATCCATTCACTCTTTCTCATGGAAGTCCCAATCACACAGCA
GTGCATGCCCACCTGGCTGGAAATACACACCTCGGAGGACAGCCTACTCTACTTCCATAC
CCATCATCAGCCACCCTCAGTAGTGCTGCACCAGTGGCCCACCTGTTAGCCTCTCCGTGT
ACCTCAAGACCTATGTTACAGCATCCAACTTATAATATCTCCCATCCCAGTGGCATAGTT
CACCAAGTCCCAGTGGGCTTAAATCCCCGTCTGTTACCATCCCCAACCATTCATCAGACT
CAGTACAAACCAATCTTCCCACCACATTCTTACATTGCAGCATCACCTGCATATACTGGA
TTTCCACTGAGTCCAACAAAACTCAGCCAGTATCCATATATGTTG

Protein sequence predicted based on nucleotide sequence

MASQVLVYPPYVYQTQSSAFCSVKKLKVEPSSCVFQERNYPRTYVNGRNFGNSHPPTKGS
AFQTKIPFNRPRGHNFSLQTSAVVLKNTAGATKVIAAQAQQAHVQAPQIGAWRNRLHFLE
GPQRCGLKRKSEELDNHSSAMQIVDELSILPAMLQTNMGNPVTVVTATTGSKQNCTTGEG
DYQLVQHEVLCSMKNTYEVLDFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQI
EVSILARLSTENADEYNFVRAYECFQHRNHTCLVFEMLEQNLYDFLKQNKFSPLPLKVIR
PILQQVATALKKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKTVCSTYL
QSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGALEYDQIRYISQTQGLPGE
QLLNVGTKSTRFFCKETDMSHSGWRLKTLEEHEAETGMKSKEARKYIFNSLDDVAHVNTV
MDLEGSDLLAEKADRREFVSLLKKMLLIDADLRITPAETLNHPFVNMKHLLDFPHSNHVK
SCFHIMDICKSHLNSCDTNNHNKTSLLRPVASSSTATLTANFTKIGTLRSQALTTSAHSV
VHHGIPLQAGTAQFGCGDAFQQTLIICPPAIQGIPATHGKPTSYSIRVDNTVPLVTQAPA
VQPLQIRPGVLSQTWSGRTQQMLVPAWQQVTPLAPATTTLTSESVAGSHRLGDWGKMISC
SNHYNSVMPQPLLTNQITLSAPQPVSVGIAHVVWPQPATTKKNKQCQNRSNSLQNTNIPH
SAFISPKIINGKDVEEVSCIETQDNQNSEGEARNCCETSIRQDSDSSVSDKQRQTIIIAD
SPSPAVSVITISSDTDEEETSQRHSLRECKGSLDCEACQSTLNIDRMCSLSSPDSTLSTS
SSGQSSPSPCKRPNSMSDEEQESSCDTVDGSPTSDSSGHDSPFAESTFVEDTHENTELVS
SADTETKPAVCSVVVPPVELENGLNADEHMANTDSICQPLIKGRSAPGRLNQPSAVGTRQ
QKLTSAFQQQHLNFSQVQHFGSGHQEWNGNFGHRRQQAYIPTSVTSNPFTLSHGSPNHTA
VHAHLAGNTHLGGQPTLLPYPSSATLSSAAPVAHLLASPCTSRPMLQHPTYNISHPSGIV
HQVPVGLNPRLLPSPTIHQTQYKPIFPPHSYIAASPAYTGFPLSPTKLSQYPYML

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
Start on reference sequence 34
End on reference sequence 3618
Mutations:
None
Discrepancy:
g207t,K69N; a3379c,K1127Q
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable