CDKL2 (Homo sapiens) in pJP1563 (retroviral expression vector)

Explanation of Terms

Gene:
Gene Symbol:  CDKL2
Gene Name:  cyclin-dependent kinase-like 2 (CDC2-related kinase)
Original Clone ID:
FLH214472.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJP1563
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HsCD00039054
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1713nts
Open reading frame: 1 to 1713

ATGGAAAAATATGAAAACCTGGGTTTGGTTGGAGAAGGGAGTTATGGAATGGTGATGAAG
TGTAGGAATAAAGATACTGGAAGAATTGTGGCCATAAAGAAGTTCTTAGAAAGTGACGAT
GACAAAATGGTTAAAAAGATTGCAATGCGAGAAATCAAGTTACTAAAGCAACTTAGGCAT
GAAAACTTGGTGAATCTCTTGGAAGTGTGTAAGAAAAAAAAACGATGGTACCTAGTCTTT
GAATTTGTTGACCACACAATTCTTGATGACTTGGAGCTCTTTCCAAATGGACTAGACTAC
CAAGTAGTTCAAAAGTATTTGTTTCAGATTATTAATGGAATTGGATTTTGTCACAGTCAC
AATATCATACACAGAGATATAAAGCCAGAGAATATATTAGTCTCCCAGTCTGGCGTTGTC
AAGCTATGCGATTTTGGATTTGCGCGAACATTGGCAGCTCCTGGGGAGGTTTATACTGAT
TATGTGGCAACCCGATGGTACAGAGCTCCAGAACTATTGGTTGGTGATGTCAAGTATGGC
AAGGCTGTTGATGTGTGGGCCATTGGTTGTCTGGTAACTGAAATGTTCATGGGGGAACCC
CTATTTCCTGGAGATTCTGATATTGATCAGCTATATCATATTATGATGTGTTTAGGTAAT
CTAATTCCAAGGCATCAGGAGCTTTTTAATAAAAATCCTGTGTTTGCTGGAGTAAGGTTG
CCTGAAATCAAGGAAATAGAACCTCTTGAAAGACGCTATCCTAAGCTCTCTGAAGTGGTG
ATAGATTTAGCAAAGAAATGCTTACATATTGACCCCGACAAAAGACCCTTCTGTGCTGAG
CTCCTACACCATGATTTCTTTCAAATGGATGGATTTGCTGAGAGGTTTTCCCAAGAACTA
CAGTTAAAAGTACAGAAAGATGCCAGAAATGTTTCTTTATCTAAAAAATCCCAAAACAGA
AAGAAGGAAAAAGAAAAAGATGATTCCTTAGTTGAAGAAAGAAAAACACTTGTGGTACAG
GATACCAATGCTGATCCCAAAATTAAGGATTATAAACTATTTAAAATAAAAGGCTCAAAA
ATTGATGGAGAAAAAGCTGAAAAAGGCAATAGAGCTTCAAATGCCAGCTGTCTCCATGAC
AGTAGGACAAGCCACAACAAAATAGTGCCTTCAACAAGCCTCAAAGACTGCAGCAATGTC
AGCGTGGACCACACAAGGAATCCAAGCGTGGCAATTCCCCCACTTACACACAATCTTTCT
GCAGTTGCTCCCAGCATTAATTCTGGAATGGGGACTGAGACTATACCAATTCAGGGTTAC
AGAGTGGATGAGAAAACTAAGAAGTGTTCTATTCCATTTGTTAAACCGAACAGACATTCC
CCATCAGGCATTTATAACATTAATGTGACCACATTAGTCTCTAGTGAAAAAAACCTCTTT
TGGGCAAGTAAGAAAAGAAGAGAATACTCCAGGACAGATGTCCGTTTGCCTGAACTAAAC
TATAATCATCTCCCTGAACTAAGAGCACTGGGAGGCATAGCTCGAAATTCCAGGCTAACA
AAGAAAGAGAGCAAAATTCTTTCAGAATCTCGAATTCCTTCTCTGGCTGCTATTGACCTG
CACACCCCCAGTATTACATTACATCAGGTATCAGGACCTCCCCTGTCAGATGATTCAGGG
GCTGATTTGCCTCAAATGGAACACCAGCACTTG

Protein sequence predicted based on nucleotide sequence

MEKYENLGLVGEGSYGMVMKCRNKDTGRIVAIKKFLESDDDKMVKKIAMREIKLLKQLRH
ENLVNLLEVCKKKKRWYLVFEFVDHTILDDLELFPNGLDYQVVQKYLFQIINGIGFCHSH
NIIHRDIKPENILVSQSGVVKLCDFGFARTLAAPGEVYTDYVATRWYRAPELLVGDVKYG
KAVDVWAIGCLVTEMFMGEPLFPGDSDIDQLYHIMMCLGNLIPRHQELFNKNPVFAGVRL
PEIKEIEPLERRYPKLSEVVIDLAKKCLHIDPDKRPFCAELLHHDFFQMDGFAERFSQEL
QLKVQKDARNVSLSKKSQNRKKEKEKDDSLVEERKTLVVQDTNADPKIKDYKLFKIKGSK
IDGEKAEKGNRASNASCLHDSRTSHNKIVPSTSLKDCSNVSVDHTRNPSVAIPPLTHNLS
AVAPSINSGMGTETIPIQGYRVDEKTKKCSIPFVKPNRHSPSGIYNINVTTLVSSEKNLF
WASKKRREYSRTDVRLPELNYNHLPELRALGGIARNSRLTKKESKILSESRIPSLAAIDL
HTPSITLHQVSGPPLSDDSGADLPQMEHQHL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1857
Start on reference sequence 376
Mutations:
None
Discrepancy:
g737t,R246I;ins@1416,231;
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable