STK40 (Homo sapiens) in pJP1563 (retroviral expression vector)

Explanation of Terms

Gene:
Gene Symbol:  STK40
Gene Name:  serine/threonine kinase 40
Original Clone ID:
FLH214484.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJP1563
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HsCD00039063
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1308nts
Open reading frame: 1 to 1308

ATGAAGCGGAGAGCATCAGACAGAGGAGCTGGGGAAACGTCGGCCAGGGCCAAGGCTCTA
GGAAGTGGGATTTCTGGAAATAATGCAAAGAGAGCTGGACCATTCATCCTTGGTCCCCGT
CTGGGCAACTCACCGGTGCCAAGCATAGTGCAGTGTTTGGCGAGGAAAGATGGCACGGAT
GACTTCTATCAGCTGAAGATCCTGACCCTGGAGGAGAGGGGGGACCAAGGCATAGAGAGC
CAGGAAGAGCGGCAGGGCAAGATGCTGCTGCACACCGAGTACTCACTGCTGTCTCTCCTG
CACACGCAGGATGGCGTGGTGCACCACCACGGCCTCTTCCAGGACCGCACCTGTGAAATC
GTTGAGGACACAGAATCCAGCCGGATGGTTAAGAAGATGAAGAAGCGCATCTGCCTCGTC
CTGGACTGCCTCTGTGCTCATGACTTCAGCGATAAGACCGCTGACCTCATCAACCTGCAG
CACTACGTCATCAAGGAGAAGAGGCTCAGCGAGAGGGAGACTGTGGTAATCTTCTACGAC
GTGGTCCGCGTGGTGGAGGCCCTGCACCAGAAAAATATCGTGCACAGAGACCTGAAGCTG
GGGAACATGGTGCTCAACAAGAGGACACATCGGATAACCATCACCAACTTCTGCCTCGGG
AAGCATCTGGTGAGCGAGGGGGACCTGCTGAAGGACCAGAGAGGGAGCCCTGCCTACATC
AGTCCCGACGTGCTCAGCGGCCGGCCGTACCGTGGCAAGCCCAGTGACATGTGGGCCCTG
GGCGTGGTGCTCTTCACCATGCTGTATGGCCAGTTCCCCTTCTACGACAGCATCCCGCAG
GAGCTCTTCCGCAAGATCAAGGCTGCCGAGTATACCATTCCTGAGGATGGACGGGTTTCT
GAGAACACCGTGTGTCTCATCCGGAAGCTGCTGGTCCTTGACCCCCAGCAGCGCCTGGCC
GCCGCCGACGTCCTGGAGGCCCTCAGTGCCATCATTGCATCATGGCAGTCCCTGTCATCT
CTGAGTGGGCCTTTGCAAGTGGTTCCTGACATTGATGACCAAATGAGCAATGCGGATAGC
TCCCAGGAGGCGAAGGTGACGGAGGAGTGCTCCCAGTACGAGTTTGAGAACTACATGCGT
CAGCAGCTGCTGCTGGCCGAGGAGAAGAGCTCCATCCATGACGCCCGGAGCTGGGTACCC
AAGCGGCAGTTTGGCAGCGCACCACCGGTGCGACGGCTGGGCCACGACGCACAGCCCATG
ACCTCCTTGGACACGGCCATCCTGGCGCAGCGCTACCTGCGGAAATTG

Protein sequence predicted based on nucleotide sequence

MKRRASDRGAGETSARAKALGSGISGNNAKRAGPFILGPRLGNSPVPSIVQCLARKDGTD
DFYQLKILTLEERGDQGIESQEERQGKMLLHTEYSLLSLLHTQDGVVHHHGLFQDRTCEI
VEDTESSRMVKKMKKRICLVLDCLCAHDFSDKTADLINLQHYVIKEKRLSERETVVIFYD
VVRVVEALHQKNIVHRDLKLGNMVLNKRTHRITITNFCLGKHLVSEGDLLKDQRGSPAYI
SPDVLSGRPYRGKPSDMWALGVVLFTMLYGQFPFYDSIPQELFRKIKAAEYTIPEDGRVS
ENTVCLIRKLLVLDPQQRLAAADVLEALSAIIASWQSLSSLSGPLQVVPDIDDQMSNADS
SQEAKVTEECSQYEFENYMRQQLLLAEEKSSIHDARSWVPKRQFGSAPPVRRLGHDAQPM
TSLDTAILAQRYLRKL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1715
Start on reference sequence 408
Mutations:
None
Discrepancy:
c1212t
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable