PIK3R4 (Homo sapiens) in pJP1563 (retroviral expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PIK3R4
Gene Name:  phosphoinositide-3-kinase, regulatory subunit 4
Original Clone ID:
FLH224151.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJP1563
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HsCD00039160
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 4077nts
Open reading frame: 1 to 4077

ATGGGAAATCAGCTTGCTGGCATTGCTCCCTCCCAGATCCTTTCTGTAGAGAGTTATTTT
TCAGATATTCATGACTTTGAATATGATAAAAGCCTGGGGAGTACTCGGTTTTTTAAAGTT
GCTCGAGCCAAGCACCGAGAAGGCCTGGTCGTTGTGAAGGTTTTTGCAATTCAGGATCCC
ACATTGCCTTTAACCAGCTATAAACAAGAGCTGGAGGAACTGAAAATCAGGCTTAATTCT
GCACAGAATTGTCTACCTTTCCAGAAAGCATCAGAAAAAGCATCTGAGAAAGCAGCTATG
CTCTTTAGGCAGTATGTGCGAGACAATCTCTATGATCGCATCAGTACCCGTCCATTCTTG
AATAACATTGAGAAGCGCTGGATTGCTTTCCAGATCCTGACAGCTGTGGACCAAGCACAC
AAATCTGGAGTTCGTCATGGGGACATCAAGACTGAGAATGTGATGGTCACCAGTTGGAAT
TGGGTTCTTCTAACTGATTTTGCCAGTTTTAAGCCCACTTATCTTCCAGAAGACAACCCG
GCAGATTTCAATTATTTCTTTGACACATCACGGAGGAGAACTTGCTATATTGCTCCTGAA
CGTTTTGTTGATGGTGGGATGTTTGCCACTGAGTTAGAATATATGAGAGATCCTTCAACT
CCGCTTGTAGACTTAAATAGCAATCAGAGAACAAGAGGAGAGTTGAAGAGAGCAATGGAC
ATCTTTTCAGCAGGTTGTGTGATAGCTGAGCTTTTTACAGAAGGTGTACCATTATTTGAT
CTCTCTCAACTTTTGGCTTATAGAAATGGACATTTTTTCCCTGAACAAGTGCTAAATAAA
ATTGAAGATCACAGTATCAGAGAATTGGTAACTCAGATGATTCACCGTGAGCCAGATAAA
CGTTTAGAGGCAGAAGATTACTTAAAACAGCAGCGTGGCAATGCCTTTCCTGACATATTT
TACACTTTTCTTCAGCCCTACATGGCCCAGTTTGCCAAGGAAACGTTTCTTTCTGCAGAT
GAGCGTATTCTGGTTATACGGAAGGATTTGGGCAACATTATTCACAATCTCTGTGGACAT
GATCTGCCAGAAAAAGCCGAAGGAGAGCCTAAGGAAAATGGGCTGGTTATCTTGGTATCT
GTTATAACATCCTGCCTACAGACCCTTAAATACTGTGATTCCAAACTAGCTGCTTTGGAA
CTGATTCTTCATTTGGCTCCAAGATTAAGTGTTGAAATCCTTTTGGATCGTATTACTCCA
TATCTTTTGCATTTCAGCAATGACTCTGTTCCTAGGGTGAGGGCTGAAGCCTTGAGGACG
TTGACCAAAGTTCTTGCTCTCGTCAAAGAGGTTCCTCGTAATGATATCAATATTTATCCG
GAATACATTCTGCCAGGCATAGCCCACTTAGCCCAAGATGATGCTACTATCGTTAGACTA
GCCTATGCTGAAAACATAGCTCTGCTGGCAGAAACAGCTCTGAGATTCCTGGAATTAGTA
CAGTTAAAAAATCTTAATATGGAAAATGACCCCAATAATGAAGAAATAGATGAGGTTACA
CATCCAAATGGAAATTATGACACAGAGCTCCAAGCCTTACATGAAATGGTCCAGCAGAAA
GTTGTTACTTTGCTAAGTGACCCTGAAAATATTGTAAAACAAACCTTGATGGAAAATGGA
ATAACACGGCTGTGTGTATTCTTTGGACGTCAGAAAGCCAACGATGTTTTGTTGTCCCAC
ATGATTACTTTCCTAAATGATAAGAATGATTGGCATCTACGTGGAGCATTTTTTGATAGT
ATAGTTGGTGTTGCTGCCTATGTTGGCTGGCAAAGCTCCTCAATTCTCAAGCCTCTGCTG
CAACAAGGTCTTAGTGATGCTGAGGAATTTGTCATTGTGAAAGCTCTTTATGCCCTTACT
TGTATGTGCCAGTTAGGACTGCTACAAAAACCCCATGTTTACGAATTTGCCAGTGATATT
GCCCCCTTCCTGTGTCATCCCAATTTATGGATACGTTATGGTGCCGTGGGATTTATCACA
GTGGTAGCTCGTCAAATAAGTACAGCTGATGTCTACTGTAAACTGATGCCTTATCTTGAC
CCATATATTACCCAACCAATAATACAGATTGAAAGAAAACTTGTTCTGCTCAGTGTTTTA
AAGGAACCAGTAAGTCGTTCTATATTTGATTATGCTTTGAGGTCTAAAGATATTACTAGC
TTGTTCAGACATCTTCACATGCGTCAGAAGAAACGAAATGGTTCTCTTCCCGACTGCCCT
CCGCCAGAGGATCCTGCCATAGCACAGCTTCTGAAGAAGTTGCTCTCACAGGGAATGACA
GAGGAAGAGGAAGACAAACTTCTGGCACTGAAAGACTTCATGATGAAATCTAATAAAGCA
AAGGCCAATATAGTGGACCAGAGCCATCTTCATGATAGTAGTCAGAAAGGTGTAATTGAC
TTGGCAGCTTTAGGCATAACTGGGAGACAAGTTGATCTTGTTAAAACCAAACAAGAACCA
GATGACAAACGGGCCAGAAAACATGTAAAACAAGACTCAAATGTAAATGAAGAATGGAAA
AGCATGTTTGGGTCACTGGACCCACCAAACATGCCACAGGCCCTACCTAAAGGGAGTGAT
CAGGAGGTGATTCAGACTGGGAAACCTCCTCGTTCCGAGTCCTCTGCTGGCATTTGTGTC
CCTTTGTCAACTTCTTCACAGGTTCCAGAAGTGACAACTGTCCAAAATAAAAAACCAGTA
ATACCGGTTTTAAGTAGTACAATCTTACCATCCACCTATCAGATTCGAATTACAACTTGT
AAAACTGAACTTCAGCAACTCATCCAGCAAAAGCGGGAGCAGTGCAATGCTGAGAGAATA
GCTAAGCAGATGATGGAAAATGCTGAATGGGAGAGTAAACCACCACCACCTGGATGGCGT
CCTAAAGGGCTGTTAGTTGCCCATCTTCATGAGCATAAATCTGCTGTGAATCGAATTAGA
GTCTCTGATGAACACTCACTTTTTGCAACATGTTCAAATGATGGCACAGTGAAAATCTGG
AACAGTCAAAAGATGGAGGGGAAGACCACCACTACCAGATCTATTCTTACATACAGCCGA
ATTGGAGGACGAGTCAAGACGCTCACATTCTGCCAAGGCTCCCACTATTTAGCCATAGCA
TCTGATAATGGTGCTGTCCAGCTTCTTGGAATTGAGGCTTCTAAGCTGCCCAAGTCTCCT
AAAATCCATCCTCTACAAAGCAGAATTCTAGATCAGAAGGAGGACGGTTGTGTTGTGGAT
ATGCATCACTTCAACTCTGGAGCACAGTCTGTTCTTGCCTATGCCACTGTGAATGGCTCT
CTGGTTGGCTGGGACCTTAGGTCTTCAAACAATGCGTGGACTTTAAAGCATGATTTAAAG
TCGGGCCTCATCACTTCCTTTGCTGTGGACATCCACCAATGCTGGCTCTGCATTGGTACA
AGCAGTGGTACCATGGCTTGTTGGGACATGAGGTTCCAGTTGCCAATTTCAAGTCACTGT
CATCCTTCCAGGGCTCGAATCAGACGCCTCTCAATGCACCCTCTGTATCAGTCCTGGGTG
ATTGCAGCTGTTCAGGGCAACAACGAAGTGTCCATGTGGGACATGGAGACTGGTGACAGA
AGATTTACTCTCTGGGCCAGCAGTGCACCACCACTTTCTGAATTACAGCCTTCTCCTCAT
AGCGTCCATGGTATCTACTGTAGTCCTGCAGATGGAAATCCTATCCTACTAACAGCTGGC
TCAGATATGAAAATAAGGTTTTGGGACTTGGCTTACCCAGAAAGGTCCTATGTTGTTGCA
GGAAGTACTAGTTCCCCATCTGTGTCCTACTACAGGAAAATAATTGAAGGCACTGAAGTT
GTCCAGGAAATTCAGAATAAGCAGAAAGTAGGACCAAGTGATGACACCCCTCGAAGGGGC
CCAGAGTCCCTGCCCGTGGGACATCATGACATCATCACTGATGTCGCCACATTCCAGACC
ACACAGGGCTTCATCGTAACTGCTTCTAGAGATGGGATTGTGAAGGTGTGGAAATTG

Protein sequence predicted based on nucleotide sequence

MGNQLAGIAPSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQDP
TLPLTSYKQELEELKIRLNSAQNCLPFQKASEKASEKAAMLFRQYVRDNLYDRISTRPFL
NNIEKRWIAFQILTAVDQAHKSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNP
ADFNYFFDTSRRRTCYIAPERFVDGGMFATELEYMRDPSTPLVDLNSNQRTRGELKRAMD
IFSAGCVIAELFTEGVPLFDLSQLLAYRNGHFFPEQVLNKIEDHSIRELVTQMIHREPDK
RLEAEDYLKQQRGNAFPDIFYTFLQPYMAQFAKETFLSADERILVIRKDLGNIIHNLCGH
DLPEKAEGEPKENGLVILVSVITSCLQTLKYCDSKLAALELILHLAPRLSVEILLDRITP
YLLHFSNDSVPRVRAEALRTLTKVLALVKEVPRNDINIYPEYILPGIAHLAQDDATIVRL
AYAENIALLAETALRFLELVQLKNLNMENDPNNEEIDEVTHPNGNYDTELQALHEMVQQK
VVTLLSDPENIVKQTLMENGITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDS
IVGVAAYVGWQSSSILKPLLQQGLSDAEEFVIVKALYALTCMCQLGLLQKPHVYEFASDI
APFLCHPNLWIRYGAVGFITVVARQISTADVYCKLMPYLDPYITQPIIQIERKLVLLSVL
KEPVSRSIFDYALRSKDITSLFRHLHMRQKKRNGSLPDCPPPEDPAIAQLLKKLLSQGMT
EEEEDKLLALKDFMMKSNKAKANIVDQSHLHDSSQKGVIDLAALGITGRQVDLVKTKQEP
DDKRARKHVKQDSNVNEEWKSMFGSLDPPNMPQALPKGSDQEVIQTGKPPRSESSAGICV
PLSTSSQVPEVTTVQNKKPVIPVLSSTILPSTYQIRITTCKTELQQLIQQKREQCNAERI
AKQMMENAEWESKPPPPGWRPKGLLVAHLHEHKSAVNRIRVSDEHSLFATCSNDGTVKIW
NSQKMEGKTTTTRSILTYSRIGGRVKTLTFCQGSHYLAIASDNGAVQLLGIEASKLPKSP
KIHPLQSRILDQKEDGCVVDMHHFNSGAQSVLAYATVNGSLVGWDLRSSNNAWTLKHDLK
SGLITSFAVDIHQCWLCIGTSSGTMACWDMRFQLPISSHCHPSRARIRRLSMHPLYQSWV
IAAVQGNNEVSMWDMETGDRRFTLWASSAPPLSELQPSPHSVHGIYCSPADGNPILLTAG
SDMKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEVVQEIQNKQKVGPSDDTPRRG
PESLPVGHHDIITDVATFQTTQGFIVTASRDGIVKVWKL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 4619
Start on reference sequence 543
Mutations:
None
Discrepancy:
a954c,E318D; g3389a,S1130N
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable