Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH230137.01X
Keyword:
None
Species:
Francisella tularensis subsp. tularensis SCHU S4
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
FtCD00039302
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4254nts
Open reading frame: 1 to 4254
ATGAATAACGGTATCCTACATCAAAATTACAATAGTAAAAAGTTTGATATTATTAAAATA
TCTTTAGCATCGCCTGAGGTTATTCGTTCGTGGTCTCATGGTGAAGTTAAAAAACCAGAA
ACTATAAACTATAGAACATTTAAGCCTGAAAGAGACGGATTATTCTGTGCTAAGATATTT
GGTCCAATAAAAGACTATGAATGCTTATGTGGTAAGTACAAAAGACTTAAGCACCGTGGC
GTAGTCTGTGAGCGTTGTGGTGTTGAGGTTGAGCAAGCTAAAGTAAGAAGAGAAAGAATG
GGGCACATTGATTTAGTATGTCCTGTAGTACATATTTGGTATCTAAAGTCTTTACCTTCT
AGAATTGGTTTATTTTTGGATATGCCATTAAAGAATGTTGAGAAGGTTTTATACTTTGAG
TCATACATAGTTACTGACCCAGGTATGACTCCATTAGAGAAAAAACAACTTCTTACAGAT
GAAGAGTATGCTGAAGCATTAGAAAATTATGGTTATGAGTTTGAAGCATCTATGGGTGCT
GAAGCAATAAGAGATTTATTGGCAGACACTGATATTGAGTCAGAGATTGAGTTACTTCAA
GCTGAATGTGAAGAGAGTAAATCTACAGCTAAGAAAGAAAAAGCTATTAAGAGATTAAGA
CTTCTTGAAACTTTCCAAGCTTCTGGTAATAAACCTGAATGGATGGTAATGACAGTACTA
CCTGTATTACCACCGGATTTAAGACCATTGGTACCAATCGAAGGTGGTAGATTTGCAACT
TCTGATCTTAATGACTTATATCGCCGAGTAATCAACAGAAACAATAGACTTAAGAAGCTA
TTGGACCTAAATGCTCCTGATATTATTGTCAGAAATGAAAAGAGAATGTTACAAGAAGCG
GTAGATGCTTTATTAGATAATGGTAGACGTGGTAGAGCAGTAACGGGCTCTAATAAAAGA
CCACTTAAATCATTAGCAGATATGATTAAGGGTAAACAAGGTCGTTTCCGTCAAAACTTA
TTAGGTAAGCGTGTTGACTATTCTGGTCGTTCTGTAATTACAGTTGGTCCATCTTTGAGA
TTACATGAGTGTGGTTTACCTAAGAAGATGGCACTAGAGTTATTTAAACCATTTGTATAC
TCTAAGCTAAGGTTAGGTGGTCATGCAACGACTATCAAACAGGCTAAGAGAATGGTTGAG
CTTGAGGAAGCTGTAGTATGGGATATTCTTGAAACTGTGATTAATGAGCATCCGGTATTA
CTAAACCGTGCTCCTACATTACATAGACTAGGTATTCAAGCGTTTGAGCCTAGACTTATT
GAAGGTAAGGCTATACAGCTACATCCATTAGTTTGTGCTGCGTTTAACGCTGACTTTGAT
GGTGACCAAATGGCTGTACACGTACCATTAACAGTTGAGTCGCAACTAGAAGCTAGAGTA
TTGATGATGTCAACAAACAACATCTTATCACCAGCATCTGGTCAACCAATTATTACTCCT
ACTCAAGATATTGTATTAGGTCTATACTATATCACTAGAGAGAAAGAAGGTGCGCGTGGC
GAGGGTAAATTATTCTCTAGCTATGAGGATGTTAGTAGAGCTTATAACTCAGGGACTATA
GATATCCATGCTAAGATTAAACTAAGAATAGATAGACAAGTATTTGATACTAAAGGTAAT
ACATACAATGAGAAAGGTGTTGTTAATACTACCGTTGGTAGAGCACTTTTATTGAATATA
CTACCAGAAGGTCTATCTTTCTCTCTATTAAATAAAGTACTAGTTAAGAAAGAAATTTCT
AAAATTATTAATCAGGCATTTAGAGTCTTAGGTGGTAAAGCTACGGTAGTTTTAGCAGAT
AAGTTGATGTATGCAGGATTTAAATATTCAACATTGTCAGGGGTATCTGTTGGTGTTGAT
GATATGACTATTCCTGATAACAAAGAAGCTAAAATTGAAGAAGCAGAAAAAGAAATTAAG
CAAATTACCGAACAGTATCAGTCTTCATTAATTACTGAAAATGAGAGATATAACAATATT
ATCAATATTTGGAGTAAGACTTCAGATGAGGTTGGAGCTTCGATGATGGATGCTATATCT
AAAGATACAGTTAGTATTAACGGTGAGAAAAAAGAAATCGAGTCATTCAACTCTGTATAT
ATGATGGCTAAATCAGGTGCAAGGGGTTCTTATAATCAGATGAGACAGCTTGCAGGTATG
CGTGGTCTGATGGCTAAGCCAGATGGTACGATGATTGAAACTGCAATTACAGCAAACTTT
AGAGAAGGTCTGTCAGTATTGCAGTACTTTACATCTACGCACGGTGCACGTAAAGGTCTA
GCTGATACGGCTCTTAAGACAGCTAACGCGGGTTATCTGACTCGTAGACTAGTTGATGTG
GCTCAAGATTTAGTTGTTATTGAAGAAGATTGTGGTACTGATGATGGTTTGATGTTCTCA
GCTATCGTTGAAGATGGTGAAGTCAAAGTTCCTCTAGTAGAGCGTGCTTTAGGAAGAACT
TTAGCAGCAGATGTGGTTACTGAGAAGGGAGTTGTATTGCTTGAAGCAGGTACTTTATTA
GATGAGAATCTTGTTGAGTTACTTGATGATAACGGTATTGATATGATCAAAGTCAGATCA
CCAATAACTTGTAAAACACGTAGAGGATTATGTGCTAAGTGTTATGGTCGTGATCTAGCG
CGTGAAAGACAAGTTAATGTTGGGGAATCTGTAGGTGTGATTGCTGCTCAATCAATTGGT
GAGCCAGGTACGCAGTTGACAATGAGAACATTCCACACTGGTGGTGCTGCGTCTCTGGGT
ATTACAGTTTCTGACATTAAAGTTAAAACTGCTGGTAAGATTAAGTTTAAGAATATTAGA
ACTGTAACTAACAAAGAAGGTCAAGAAATTGTTATATCTCGCGCAGGTGAGATTATCGTT
TCTGATACTATGGGTAGAGTGAGAGAACAGCATAAGATCCCTATGGGTGCAGTCGTTCCT
TTAGCTAGTGGTAAAGCAGTAGAAATTGGTGATGTGATTGCTACGTGGGATCCGCATGCT
CAGCCATTAATTACAGATGTTGCTGGTAAAGTTGTTCTTGAAGATGTGATTGATGGTATA
ACATCTAAGCATACTTATGATGATTTGACTGGTCAGCAAACTATTGAGATAACTTCAATA
TCTCAAAGAACAACATCTAAAAACTTAAAACCAGTTGTTAAGATTGTTGATGAAAAGGGT
GCCGAGCTTAAGTCAATACCTCTAGCAGTTGGAGCAGTACTAAACGTTGCCGATGATTCT
ATACTAGAAGTTGGTGATATCGTAGCTAAGATTCCTCTAGAAGGTTCTAAGAACAAAGAT
ATTACCGGGGGTCTTCCACGTGTTGCTGAGCTTTTCGAAGCTAGACGTCCTAAAGATGCG
GCAATACTTTCTCCATGTGATGGTATGGTTAGGCTTGGTAACAGAGACACTAAAGAAAAA
CAGCGTATTGAGATTATAGATAAAAATGGTCATATTGTTGAAGAGATATTATTACCTAAG
TCTCGTCACCTAGTAGTTTTTGATGGTGAGCAGGTTTCTAGGGGTGATGTTTTAGCTGAT
GGACCTACTGATCCACATGATCTTCTTAAGTACAAAGGACTTGAGGAATTTGCTGATTAT
ATTCTTATCGAAGCGCAGTCTGTATATCGTATGCAGGGTGTTGTTATTAATGATAAGCAT
ATTGAAACAATTGTAAGACAAATGTTAAGAAAAGCTGTAATCCTTGATGAAGGTGATAGT
AAGTTTGTCAAAGATGAAAGTATTGAGTTAGTAAGAATACTTGAAGAAAATGATAAACTA
CGTAAGCAAGGTAAAAAAGAAGTAGAGTATGAGCTGGTATTAATGGGTATTACACGCTCT
TCTTTATCTACGGAGTCATTCTTATCGGCTGCTTCTTTCCAAGAAACAACAAGAGTGCTT
ACTGAGGCTTCGATAAACTCACAAATTGATAATTTAAGAGGACTTAAAGAAAATGTTCTT
ATTGGTAGATTAATACCAACAGGTACTGGTCTAGCAGTAAGAAAAGAGTCAGCTAAAATT
GAAAAAATGCGTGAAGAATTAGGGGTTGAAGATAATATGGTGTTTACTGATTTATCGTCT
TTTAACCCTGAAGAAATCTCTTTTGATAGTATTCAATCTCAAAAAGAAGATAAAGATATT
AATGAAGACATCGAGGAGTCATTAAGAAACGCTCTTGAGTCACTTGATTTCTAG
Protein sequence predicted based on nucleotide sequence
MNNGILHQNYNSKKFDIIKISLASPEVIRSWSHGEVKKPETINYRTFKPERDGLFCAKIF GPIKDYECLCGKYKRLKHRGVVCERCGVEVEQAKVRRERMGHIDLVCPVVHIWYLKSLPS RIGLFLDMPLKNVEKVLYFESYIVTDPGMTPLEKKQLLTDEEYAEALENYGYEFEASMGA EAIRDLLADTDIESEIELLQAECEESKSTAKKEKAIKRLRLLETFQASGNKPEWMVMTVL PVLPPDLRPLVPIEGGRFATSDLNDLYRRVINRNNRLKKLLDLNAPDIIVRNEKRMLQEA VDALLDNGRRGRAVTGSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGPSLR LHECGLPKKMALELFKPFVYSKLRLGGHATTIKQAKRMVELEEAVVWDILETVINEHPVL LNRAPTLHRLGIQAFEPRLIEGKAIQLHPLVCAAFNADFDGDQMAVHVPLTVESQLEARV LMMSTNNILSPASGQPIITPTQDIVLGLYYITREKEGARGEGKLFSSYEDVSRAYNSGTI DIHAKIKLRIDRQVFDTKGNTYNEKGVVNTTVGRALLLNILPEGLSFSLLNKVLVKKEIS KIINQAFRVLGGKATVVLADKLMYAGFKYSTLSGVSVGVDDMTIPDNKEAKIEEAEKEIK QITEQYQSSLITENERYNNIINIWSKTSDEVGASMMDAISKDTVSINGEKKEIESFNSVY MMAKSGARGSYNQMRQLAGMRGLMAKPDGTMIETAITANFREGLSVLQYFTSTHGARKGL ADTALKTANAGYLTRRLVDVAQDLVVIEEDCGTDDGLMFSAIVEDGEVKVPLVERALGRT LAADVVTEKGVVLLEAGTLLDENLVELLDDNGIDMIKVRSPITCKTRRGLCAKCYGRDLA RERQVNVGESVGVIAAQSIGEPGTQLTMRTFHTGGAASLGITVSDIKVKTAGKIKFKNIR TVTNKEGQEIVISRAGEIIVSDTMGRVREQHKIPMGAVVPLASGKAVEIGDVIATWDPHA QPLITDVAGKVVLEDVIDGITSKHTYDDLTGQQTIEITSISQRTTSKNLKPVVKIVDEKG AELKSIPLAVGAVLNVADDSILEVGDIVAKIPLEGSKNKDITGGLPRVAELFEARRPKDA AILSPCDGMVRLGNRDTKEKQRIEIIDKNGHIVEEILLPKSRHLVVFDGEQVSRGDVLAD GPTDPHDLLKYKGLEEFADYILIEAQSVYRMQGVVINDKHIETIVRQMLRKAVILDEGDS KFVKDESIELVRILEENDKLRKQGKKEVEYELVLMGITRSSLSTESFLSAASFQETTRVL TEASINSQIDNLRGLKENVLIGRLIPTGTGLAVRKESAKIEKMREELGVEDNMVFTDLSS FNPEEISFDSIQSQKEDKDINEDIEESLRNALESLDF*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
4254
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
